Programa de intervención en regulación emocional centrado en la función reflexiva de las familias

El presente trabajo muestra la importancia que tiene el primer contexto de socialización como es la familia en la regulación de las emociones de sus hijos/as para el desarrollo de su completo bienestar psicológico. Por este motivo, se considera necesario trabajar con las familias un concepto innovador que ha sido estudiado e investigado en las últimas publicaciones recientes; Mentalización o Función reflexiva, siendo estos la capacidad de comprender los propios estados mentales y el de los demás, como son: las intenciones, sentimientos, pensamientos, deseos y creencias. Valorando la necesidad psicoeducativa, se ha diseñado un Programa de Intervención en Regulación Emocional dirigido a familias con el objetivo primordial de que éstas puedan ayudar a sus hijos/as a la regulación de sus emociones, al mismo tiempo que trabajemos con ellas la autoconciencia emocional, regulación emocional, autorreflexión, mentalización; a través de catorce sesiones teórico-prácticas donde diseñamos dinámicas útiles y divertidas, así como técnicas de respiración y diversos tipos de relajación con el fin de adquirir estrategias y habilidades queriendo conseguir la máxima participación y rendimiento de los participantes. Finalmente, las familias serán evaluadas a través de cuestionarios de Regulación Emocional y Mentalización con la finalidad de corroborar la eficacia del programa.The present work shows the importance of the first socialization context such as the family in the regulation of their children's emotions for the development of their complete psychological well-being. For this reason, it is considered necessary to work with families, an innovative concept that has been studied and researched in recent publications; Mentalization or Reflective Function, being these the capacity to understand one's own mental states and those of others, such as: intentions, feelings, thoughts, desires and beliefs. Valuing the psychoeducational need, we have designed an Intervention Program in Emotional Regulation addressed to families with the primary objective that they can help their children to regulate their emotions, while working with them the emotional self-awareness, emotional regulation, self-reflection, mentalization; through thirteen theoretical and practical sessions where we design useful and fun dynamics, as well as breathing techniques and various types of relaxation in order to acquire strategies and skills wanting to achieve maximum participation and performance of the participants. Finally, the families will be evaluated through questionnaires of Emotional Regulation and Mentalization with the purpose of corroborating the effectiveness of the program.Psicologí

TRADE (MLAP93/003)

La Traducción Automática se considera una de las aplicaciones más importantes de la Ingeniería Lingüística, desde el punto de vista comercial. A pesar de que el problema de la TA está lejos de haber sido resuelto, se pone de manifiesto la necesidad de disponer de productos operativos que cubran, al menos parcialmente, la demanda del mercado en este sentido, proporcionando ayudas y herramientas para la traducción..

Effects of Fish Oil and Grape Seed Extract Combination on Hepatic Endogenous Antioxidants and Bioactive Lipids in Diet-Induced Early Stages of Insulin Resistance in Rats

© 2020 by the authors.Diacylglycerols (DAG) and ceramides have been suggested as early predictors of insulin resistance. This study was aimed to examine the combined effects of fish oil (FO) and grape seed extract (GSE) on hepatic endogenous antioxidants, DAG and ceramides in diet-induced early stages of insulin resistance. Thirty-five rats were fed one of the following diets: (1) a standard diet (STD group), (2) a high-fat high-sucrose diet (HFHS group), (3) an HFHS diet enriched with FO (FO group), (4) an HFHS diet enriched with GSE (GSE group) or (5) an HFHS diet enriched with FO and GSE (FO + GSE group). In the liver, endogenous antioxidants were measured using spectrophotometric and fluorometric techniques, and non-targeted lipidomics was conducted for the assessment of DAG and ceramides. After 24 weeks, the FO + GSE group showed increased glutathione peroxidase activity, as well as monounsaturated fatty acid and polyunsaturated fatty acid-containing DAG, and long-chain fatty acid-containing ceramides abundances compared to the STD group. The FO and GSE combination induced similar activation of the antioxidant system and bioactive lipid accumulation in the liver than the HFHS diet without supplementation. In addition, the FO and GSE combination increased the abundances of polyunsaturated fatty acid-containing DAG in the liver.This work was supported by the Spanish Ministry of Economy and Competitiveness [grant numbers AGL2009-12374-C03-02, AGL2013-49079-C2-1-R and AGL2013-49079-C2-2-R].Peer reviewe

Effects of fish oil and grape seed extract combination on hepatic endogenous antioxidants and bioactive lipids in diet-induced early stages of insulin resistance in rats

Diacylglycerols (DAG) and ceramides have been suggested as early predictors of insulinresistance. This study was aimed to examine the combined effects of fish oil (FO) and grape seedextract (GSE) on hepatic endogenous antioxidants, DAG and ceramides in diet-induced early stagesof insulin resistance. Thirty-five rats were fed one of the following diets: (1) a standard diet (STDgroup), (2) a high-fat high-sucrose diet (HFHS group), (3) an HFHS diet enriched with FO (FO group),(4) an HFHS diet enriched with GSE (GSE group) or (5) an HFHS diet enriched with FO and GSE(FO+GSE group). In the liver, endogenous antioxidants were measured using spectrophotometricand fluorometric techniques, and non-targeted lipidomics was conducted for the assessment of DAGand ceramides. After 24 weeks, the FO+GSE group showed increased glutathione peroxidaseactivity, as well as monounsaturated fatty acid and polyunsaturated fatty acid-containing DAG, andlong-chain fatty acid-containing ceramides abundances compared to the STD group. The FO and GSEcombination induced similar activation of the antioxidant system and bioactive lipid accumulation inthe liver than the HFHS diet without supplementation. In addition, the FO and GSE combinationincreased the abundances of polyunsaturated fatty acid-containing DAG in the liver

Genetics and Genomics of SOST: functional analysis of variants and genomic regulation in osteoblasts

SOST encodes the sclerostin protein, which acts as a key extracellular inhibitor of the canonical Wnt pathway in bone, playing a crucial role in skeletal development and bone homeostasis. The objective of this work was to assess the functionality of two variants previously identified (the rare variant rs570754792 and the missense variant p.Val10Ile) and to investigate the physical interactors of the SOST proximal promoter region in bone cells. Through a promoter luciferase reporter assay we show that the minor allele of rs570754792, a variant located in the extended TATA box motif, displays a significant decrease in promoter activity. Likewise, through western blot studies of extracellular and intracellular sclerostin, we observe a reduced expression of the p.Val10Ile mutant protein. Finally, using a circular chromosome conformation capture assay (4C-seq) in 3 bone cell types (MSC, hFOB, Saos-2), we have detected physical interactions between the SOST proximal promoter and the ECR5 enhancer, several additional enhancers located between EVT4 and MEOX1 and a distant region containing exon 18 of DHX8. In conclusion, SOST presents functional regulatory and missense variants that affect its expression and displays physical contacts with far reaching genomic sequences, which may play a role in its regulation within bone cells

In-vitro comparison of hydroxyapatite coatings obtained by cold spray and conventional thermal spray technologies

Hydroxyapatite (HA) coatings onto Ti6Al4V alloy substrates were obtained by several thermal spray technologies: atmospheric plasma spray (APS) and high velocity oxy fuel (HVOF), together with the cold spray (CS) technique. A characterization study has been performed by means of surface and microstructure analyses, as well as biological performance. In-vitro tests were performed with primary human osteoblasts at 1, 7 and 14 days of cell culture on substrates. Cell viability was tested by MTS and LIVE/DEAD assays, cell differentiation by alkaline phosphatase (ALP) quantification, and cell morphology was analyzed by scanning electron microscopy. The HA coatings showed an increase of HA crystallinity from 62,4% to 89%, but also an increase of hydrophilicity from ∼32° to 0°, with the decrease of the operating temperature of the thermal spray techniques (APS > HVOF > CS). Additionally, APS HA coatings showed more surface micro-features than HVOF and CS HA coatings; cells onto APS HA coatings showed faster attachment by acquiring osteoblastic morphology in comparison with the rounded cell morphology observed onto CS HA coatings at 1 day of cell culture. HVOF HA coatings also showed proper cell adherence but did not show extended filopodia as cells onto APS HA coatings. However, at 14 days of cell culture, higher cell proliferation and differentiation was detected on HA coatings with higher crystallinity (HVOF and CS techniques). Cell attachment is suggested to be favoured by surface micro-features but also moderate surface wettability whereas cell proliferation and differentiation is suggested to be highly influenced by HA crystallinity and crystal size

Functionalized coatings by cold spray: an in vitro study of micro- and nanocrystalline hydroxyapatite compared to porous titanium

Three different surface treatments on a Ti6Al4V alloy have been in vitro tested for possible application in cementless joint prosthesis. All of them involve the novelty of using the Cold Spray technology for their deposition: (i) an as-sprayed highly rough titanium and, followed by the deposition of a thin hydroxyapatite layer with (ii) microcrystalline or (iii) nanocrystalline structure. Primary human osteoblasts were extracted from knee and seeded onto the three different surfaces. Cell viability was tested by MTS and LIVE/DEAD assays, cell differentiation by alkaline phosphatase (ALP) quantification and cell morphology by Phalloidin staining. All tests were carried out at 1, 7 and 14 days of cell culture. Different cell morphologies between titanium and hydroxyapatite surfaces were exhibited. At 1 day of cell culture, cells on the titanium coating were spread and flattened, expanding the filopodia actin filaments in all directions, while cells on the hydroxyapatite coatings showed round like-shape morphology due to slower attachment. Higher cell viability was detected at all times of cell culture on titanium coating due to a better attachment at 1 day. However, from 7 days of cell culture, cells on hydroxyapatite showed good attachment onto surfaces and highly increased their proliferation, mostly on nanocrystalline, achieving similar cell viability levels than titanium coatings. ALP levels were significantly higher in titanium, in part, because of greatest cell number. Overall, the best cell functional results were obtained on titanium coatings whereas microcrystalline hydroxyapatite presented the worst cellular parameters. However, results indicate that nanocrystalline hydroxyapatite coatings may achieve promising results for the faster cell proliferation once cells are attached on the surface