Fatigue strength evaluation for bolt-nut connections having slight pitch difference considering incomplete threads of nut

Proceedings of 4th International Conference on Fracture Fatigue and Wear, FFW 2015, Ghent University, Belgium, 27-28 August 2015The high strength bolts and nuts are widely used in various fields. In this study the effect of slight pitch difference is considered when the nut pitch is αμm larger than the bolt pitch. In the first place, the fatigue experiment is conducted with varying pitch difference. The results show that the fatigue life is extended to about 1.5 times by introducing the suitable pitch difference under the high stress amplitude. Next, the detail observation is performed on the fractured specimens including the fractured positions and the crack configurations. It is found that the fractured positions and the crack distributions vary depending on the pitch difference. Finally, to clarify the improvement mechanism of the fatigue strength, the finite element method is applied to calculate the stress amplitude and mean stress at each bolt threads, and the incomplete threads at the nut ends are also considered to obtain the accurate analytical results

Fatigue life improvement by slight pitch difference in bolt-nut connections

The bolt-nut connections are widely used in various fields. In this paper, a slight pitch difference is introduced between the bolt and nut in order to study the effect on the fatigue performance. Here, we consider that the nut pitch is a few microns larger than the bolt pitch. Fatigue experiments are conducted for three kinds of specimens with different levels of pitch differences. The obtained S-N curves show that the fatigue life is extended to about 1.5 times by introducing a suitable pitch difference. According to the detailed investigation on the fractured specimens, it is found that the fractured positions and the crack configuration are totally different depending on the pitch difference. The mechanism of the fatigue strength improvement is discussed in terms of the stress amplitude and average stress at each bolt thread

Fatigue strength evaluation for bolt-nut connections having slight pitch difference considering incomplete threads of nut

The high strength bolts and nuts are widely used in various fields. In this study the effect of slightpitch difference is considered when the nut pitch is αμm larger than the bolt pitch. In the first place, the fatigue experiment is conducted with varying pitch difference. The results show that the fatigue life isextended to about 1.5 times by introducing the suitable pitch difference under the high stress amplitude.Next, the detail observation is performed on the fractured specimens including the fractured positions andthe crack configurations. It is found that the fractured positions and the crack distributions vary depending on the pitch difference. Finally, to clarify the improvement mechanism of the fatigue strength, the finite element method is applied to calculate the stress amplitude and mean stress at each bolt threads, and theincomplete threads at the nut ends are also considered to obtain the accurate analytical results.Proceedings of 4th International Conference on Fracture Fatigue and Wear, FFW 2015, Ghent University, Belgium, 27-28 August 201

Effect of Pitch Difference between the Bolt-Nut Connections upon the Anti-Loosening Performance and Fatigue Life

In this paper, the effect of a slight pitch difference between a bolt and nut is studied. Firstly, by varying the pitch difference, the prevailing torque required for the nut rotation, before the nut touches the clamped body, is measured experimentally. Secondly, the tightening torque is determined as a function of the axial force of the bolt after the nut touches the clamped body. The results show that a large value of pitch difference may provide large prevailing torque that causes an anti-loosening effect although a very large pitch difference may deteriorate the bolt axial force under a certain tightening torque. Thirdly, a suitable pitch difference is determined taking into account the anti-loosening and clamping abilities. Fourthly, fatigue experiments are conducted using three different values of pitch difference for various stress amplitudes. It is found that the fatigue life could be extended when a suitable pitch difference is considered Furthermore, the chamfered corners at nut ends are considered, and it is found that the finite element analysis with considering the chamfered nut threads has a good agreement with the experimental observation. Finally, the most desirable pitch difference required for improving both anti-loosening and fatigue life is proposed

微小螺距差對螺栓螺母聯接件的疲勞壽命的影響

The bolt-nut connections are widely used in various fields. In this paper, a slight pitch difference is introduced between the bolt and nut in order to study the effect on the fatigue performance. Here, we consider that the nut pitch is a few microns larger than the bolt pitch. Fatigue experiments are conducted for three kinds of specimens with different levels of pitch differences. The obtained S-N curves show that the fatigue life is extended to about 1.5 times by introducing a suitable pitch difference. According to the detailed investigation on the fractured specimens, it is found that the fractured positions and the crack configuration are totally different depending on the pitch difference. The mechanism of the fatigue strength improvement is discussed in terms of the stress amplitude and average stress at each bolt thread. / 本文旨在探討微小螺距差對螺栓螺母聯接件的疲勞壽命的影響. 通過增大螺母的螺距使得螺紋之間的接觸狀況發生變化. 疲勞試驗結果顯示合理的螺距差可以大幅度提高螺栓的疲勞壽命,螺栓也呈現出不同的疲勞破壞形態. 通過對試件的裂紋觀察，發現不同的螺距差導致螺栓在疲勞破話過程中產生不同形態的裂紋

Phamacogenomics of Clozapine-Induced Agranulocytosis

Background: Clozapine-induced agranulocytosis (CIA)/clozapine-induced granulocytopenia (CIG) (CIAG) is a life-threatening event for schizophrenic subjects treated with clozapine. Methods: To examine the genetic factor for CIAG, a genome-wide pharmacogenomic analysis was conducted using 50 subjects with CIAG and 2905 control subjects. Results: We identified a significant association in the human leukocyte antigen (HLA) region (rs1800625, p = 3.46 × 10−9, odds ratio [OR] = 3.8); therefore, subsequent HLA typing was performed. We detected a significant association of HLA-B*59:01 with CIAG (p = 3.81 × 10−8, OR = 10.7) and confirmed this association by comparing with an independent clozapine-tolerant control group (n = 380, p = 2.97 × 10−5, OR = 6.3). As we observed that the OR of CIA (OR: 9.3~15.8) was approximately double that in CIG (OR: 4.4~7.4), we hypothesized that the CIG subjects were a mixed population of those who potentially would develop CIA and those who would not develop CIA (non-CIA). This hypothesis allowed the proportion of the CIG who were non-CIA to be calculated, enabling us to estimate the positive predictive value of the nonrisk allele on non-CIA in CIG subjects. Assuming this model, we estimated that 1) ~50% of CIG subjects would be non-CIA; and 2) ~60% of the CIG subjects without the risk allele would be non-CIA and therefore not expected to develop CIA. Conclusions: Our results suggest that HLA-B*59:01 is a risk factor for CIAG in the Japanese population. Furthermore, if our model is true, the results suggest that rechallenging certain CIG subjects with clozapine may not be always contraindicated

EGUIDE project and treatment guidelines

Aim: Although treatment guidelines for pharmacological therapy for schizophrenia and major depressive disorder have been issued by the Japanese Societies of Neuropsychopharmacology and Mood Disorders, these guidelines have not been well applied by psychiatrists throughout the nation. To address this issue, we developed the ‘Effectiveness of Guidelines for Dissemination and Education in Psychiatric Treatment (EGUIDE)’ integrated education programs for psychiatrists to disseminate the clinical guidelines. Additionally, we conducted a systematic efficacy evaluation of the programs. Methods: Four hundred thirteen out of 461 psychiatrists attended two 1‐day educational programs based on the treatment guidelines for schizophrenia and major depressive disorder from October 2016 to March 2018. We measured the participants’ clinical knowledge of the treatment guidelines using self‐completed questionnaires administered before and after the program to assess the effectiveness of the programs for improving knowledge. We also examined the relation between the participants’ demographics and their clinical knowledge scores. Results: The clinical knowledge scores for both guidelines were significantly improved after the program. There was no correlation between clinical knowledge and participant demographics for the program on schizophrenia; however, a weak positive correlation was found between clinical knowledge and the years of professional experience for the program on major depressive disorder. Conclusion: Our results provide evidence that educational programs on the clinical practices recommended in guidelines for schizophrenia and major depressive disorder might effectively improve participants’ clinical knowledge of the guidelines. These data are encouraging to facilitate the standardization of clinical practices for psychiatric disorders

On the origin and evolution of the asteroid Ryugu: A comprehensive geochemical perspective

Presented here are the observations and interpretations from a comprehensive analysis of 16 representative particles returned from the C-type asteroid Ryugu by the Hayabusa2 mission. On average Ryugu particles consist of 50% phyllosilicate matrix, 41% porosity and 9% minor phases, including organic matter. The abundances of 70 elements from the particles are in close agreement with those of CI chondrites. Bulk Ryugu particles show higher δ18O, Δ17O, and ε54Cr values than CI chondrites. As such, Ryugu sampled the most primitive and least-thermally processed protosolar nebula reservoirs. Such a finding is consistent with multi-scale H-C-N isotopic compositions that are compatible with an origin for Ryugu organic matter within both the protosolar nebula and the interstellar medium. The analytical data obtained here, suggests that complex soluble organic matter formed during aqueous alteration on the Ryugu progenitor planetesimal (several 10’s of km), <2.6 Myr after CAI formation. Subsequently, the Ryugu progenitor planetesimal was fragmented and evolved into the current asteroid Ryugu through sublimation

The whole blood transcriptional regulation landscape in 465 COVID-19 infected samples from Japan COVID-19 Task Force

「コロナ制圧タスクフォース」COVID-19患者由来の血液細胞における遺伝子発現の網羅的解析 --重症度に応じた遺伝子発現の変化には、ヒトゲノム配列の個人差が影響する--. 京都大学プレスリリース. 2022-08-23.Coronavirus disease 2019 (COVID-19) is a recently-emerged infectious disease that has caused millions of deaths, where comprehensive understanding of disease mechanisms is still unestablished. In particular, studies of gene expression dynamics and regulation landscape in COVID-19 infected individuals are limited. Here, we report on a thorough analysis of whole blood RNA-seq data from 465 genotyped samples from the Japan COVID-19 Task Force, including 359 severe and 106 non-severe COVID-19 cases. We discover 1169 putative causal expression quantitative trait loci (eQTLs) including 34 possible colocalizations with biobank fine-mapping results of hematopoietic traits in a Japanese population, 1549 putative causal splice QTLs (sQTLs; e.g. two independent sQTLs at TOR1AIP1), as well as biologically interpretable trans-eQTL examples (e.g., REST and STING1), all fine-mapped at single variant resolution. We perform differential gene expression analysis to elucidate 198 genes with increased expression in severe COVID-19 cases and enriched for innate immune-related functions. Finally, we evaluate the limited but non-zero effect of COVID-19 phenotype on eQTL discovery, and highlight the presence of COVID-19 severity-interaction eQTLs (ieQTLs; e.g., CLEC4C and MYBL2). Our study provides a comprehensive catalog of whole blood regulatory variants in Japanese, as well as a reference for transcriptional landscapes in response to COVID-19 infection

DOCK2 is involved in the host genetics and biology of severe COVID-19

「コロナ制圧タスクフォース」COVID-19疾患感受性遺伝子DOCK2の重症化機序を解明 --アジア最大のバイオレポジトリーでCOVID-19の治療標的を発見--. 京都大学プレスリリース. 2022-08-10.Identifying the host genetic factors underlying severe COVID-19 is an emerging challenge. Here we conducted a genome-wide association study (GWAS) involving 2, 393 cases of COVID-19 in a cohort of Japanese individuals collected during the initial waves of the pandemic, with 3, 289 unaffected controls. We identified a variant on chromosome 5 at 5q35 (rs60200309-A), close to the dedicator of cytokinesis 2 gene (DOCK2), which was associated with severe COVID-19 in patients less than 65 years of age. This risk allele was prevalent in East Asian individuals but rare in Europeans, highlighting the value of genome-wide association studies in non-European populations. RNA-sequencing analysis of 473 bulk peripheral blood samples identified decreased expression of DOCK2 associated with the risk allele in these younger patients. DOCK2 expression was suppressed in patients with severe cases of COVID-19. Single-cell RNA-sequencing analysis (n = 61 individuals) identified cell-type-specific downregulation of DOCK2 and a COVID-19-specific decreasing effect of the risk allele on DOCK2 expression in non-classical monocytes. Immunohistochemistry of lung specimens from patients with severe COVID-19 pneumonia showed suppressed DOCK2 expression. Moreover, inhibition of DOCK2 function with CPYPP increased the severity of pneumonia in a Syrian hamster model of SARS-CoV-2 infection, characterized by weight loss, lung oedema, enhanced viral loads, impaired macrophage recruitment and dysregulated type I interferon responses. We conclude that DOCK2 has an important role in the host immune response to SARS-CoV-2 infection and the development of severe COVID-19, and could be further explored as a potential biomarker and/or therapeutic target