4,288 research outputs found
Effects of high-intensity interval walking training on physical fitness and blood pressure in middle-aged and older people
Reproduced from Mayo Clin Proc., with permission), permission is hereby granted to place a pdf of Nemoto, K et al. Effects of high-intensity interval walking training on physical fitness and blood pressure in middle-aged and older people. Mayo Clin Proc. 82 (7):803-811 into the institutional repository of Shinshu University at https://soar-ir.shinshu-u.ac.jp/ArticleMAYO CLINIC PROCEEDINGS. 82(7): 803-811 (2007)journal articl
Bisphosphonate inhibits the expression of cyclin A2 at the transcriptional level in normal human oral keratinocytes.
Nitrogen-containing bisphosphonates (N-BPs) are the most widely used anti-resorptive agents in the treatment of bone-related diseases. N-BPs inhibit bone resorption by specifically targeting osteoclasts, bone-resorbing cells. However, soft tissue toxicity, such as oral or gastrointestinal (GI) ulcerations has frequently been reported in N-BP users, suggesting that N-BPs may also directly target cells other than osteoclasts. Previously, we reported that BPs inhibit proliferation without inducing the apoptosis of normal human oral keratinocytes (NHOKs). However, the molecular mechanisms through which N-BPs inhibit the proliferation of NHOKs are not yet fully understood. In this study, we performed gene expression profiling in N-BP-treated NHOKs and identified cyclin A2 as one of the most commonly downregulated genes. When the NHOKs were treated with N-BPs, we found that the level of cyclin A2 was suppressed in a dose- and time-dependent manner. In addition, the protein level of cyclin A2 was also significantly lower in oral epithelial cells in N-BP-treated oral mucosal tissue constructs. Cyclin A2 promoter reporter assay revealed that N-BPs inhibited the luciferase activity, indicating that the inhibition of cyclin A2 expression occurs at the transcriptional level. Furthermore, N-BPs did not alter the expression of cyclin A2 in normal human oral fibroblasts (NHOFs), suggesting that the effect of N-BPs on cyclin A2 expression may be cell-type specific. Thus, the findings of our study demonstrate that the inhibition of NHOK proliferation by N-BPs is mediated, at least in part, by the suppression of cyclin A2 expression at the transcriptional level, which may explain the underlying mechanisms of soft tissue toxicity by N-BPs
Energy loss mechanism for suspended micro- and nanoresonators due to the Casimir force
A so far not considered energy loss mechanism in suspended micro- and
nanoresonators due to noncontact acoustical energy loss is investigated
theoretically. The mechanism consists on the conversion of the mechanical
energy from the vibratory motion of the resonator into acoustic waves on large
nearby structures, such as the substrate, due to the coupling between the
resonator and those structures resulting from the Casimir force acting over the
separation gaps. Analytical expressions for the resulting quality factor Q for
cantilever and bridge micro- and nanoresonators in close proximity to an
underlying substrate are derived and the relevance of the mechanism is
investigated, demonstrating its importance when nanometric gaps are involved
The Effects of Cellulase on Cell Wall Structure and the Rumen Digestion of Alfalfa Silage
First- and second-cut alfalfa (Medicago sativa) was ensiled with no additive, microbial (Lactobacillus casei) inoculant, cellulase derived from Acremonium celluloytics Y-94, co-addition of inoculant and cellulase, and formic acid. The resultant silages were digested in the rumen of a dairy cow. The alfalfa and the silages were then examined with scanning electron microscope (SEM) and their chemical characteristics analyzed to evaluate the effects of cellulase on the quality of alfalfa silage and its cell wall structure.
The addition of cellulase lend to both a greater loss of parenchymal tissue and decrease in digestibility during rumen degradation than did the other additives moreover, photos taken during SEM examination also showed that cellulase affected cell wall decomposition. The results of this study may suggest that the addition of cellulase affects fiber digestion by ruminant animals
Interaction and Localization of One-electron Orbitals in an Organic Molecule: Fictitious Parameter Analysis for Multi-physics Simulations
We present a new methodology to analyze complicated multi-physics simulations
by introducing a fictitious parameter. Using the method, we study quantum
mechanical aspects of an organic molecule in water. The simulation is
variationally constructed from the ab initio molecular orbital method and the
classical statistical mechanics with the fictitious parameter representing the
coupling strength between solute and solvent. We obtain a number of
one-electron orbital energies of the solute molecule derived from the
Hartree-Fock approximation, and eigenvalue-statistical analysis developed in
the study of nonintegrable systems is applied to them. Based on the results, we
analyze localization properties of the electronic wavefunctions under the
influence of the solvent.Comment: 4 pages, 5 figures, the revised version will appear in J. Phys. Soc.
Jpn. Vol.76 (No.1
Chiral perturbation theory in a magnetic background - finite-temperature effects
We consider chiral perturbation theory for SU(2) at finite temperature in
a constant magnetic background . We compute the thermal mass of the pions
and the pion decay constant to leading order in chiral perturbation theory in
the presence of the magnetic field. The magnetic field gives rise to a
splitting between and as well as between
and . We also calculate the free energy and the
quark condensate to next-to-leading order in chiral perturbation theory. Both
the pion decay constants and the quark condensate are decreasing slower as a
function of temperature as compared to the case with vanishing magnetic field.
The latter result suggests that the critical temperature for the chiral
transition is larger in the presence of a constant magnetic field. The increase
of as a function of is in agreement with most model calculations but
in disagreement with recent lattice calculations.Comment: 24 pages and 9 fig
Chamber winds : Party music
Adam GorbErik SatieHK Grube
SIGNALS FOR MINIMAL SUPERGRAVITY AT THE CERN LARGE HADRON COLLIDER: MULTI-JET PLUS MISSING ENERGY CHANNEL,
We use ISAJET to perform a detailed study of the missing transverse energy
\eslt plus multi-jet signal expected from superparticle production at the
CERN LHC. Our analysis is performed within the framework of the minimal
supergravity model with gauge coupling unification and radiative electroweak
symmetry breaking. We delineate the region of parameter space where the \eslt
supersymmetry signal should be observable at the LHC and compare it to the
regions explorable via searches for sleptons and for chargino/neutralino
production. We confirm that, given a data sample of 10~\fb^{-1}, GeV can be explored if m_{\tq}\gg m_{\tg}, while GeV
can be probed if m_{\tq}\simeq m_{\tg}. We further examine what information
can be gleaned from scrutinizing this event sample. For instance, the multi-jet
multiplicity yields information on whether squark production makes a
significant contribution to the observed \eslt sample. Furthermore,
reconstructing hemispheric masses may yield a measure of to . Finally, for favourable ranges of parameters, by reconstructing
masses of tagged jet pairs, it may be possible to detect Higgs
bosons produced via sparticle cascade decay chains.Comment: 22 pages (REVTEX); a PS text file (etmiss.ps) and 12 figures
(etlhc.uu or etlhc.ps) can be obtained via anonymous ftp at
ftp://hep.fsu.edu/anonymous.bae
Wnt5a induces ROR1 to associate with 14-3-3ζ for enhanced chemotaxis and proliferation of chronic lymphocytic leukemia cells.
Wnt5a can activate Rho GTPases in chronic lymphocytic leukemia (CLL) cells by inducing the recruitment of ARHGEF2 to ROR1. Mass spectrometry on immune precipitates of Wnt5a-activated ROR1 identified 14-3-3ζ, which was confirmed by co-immunoprecipitation. The capacity of Wnt5a to induce ROR1 to complex with 14-3-3ζ could be blocked in CLL cells by treatment with cirmtuzumab, a humanized mAb targeting ROR1. Silencing 14-3-3ζ via small interfering RNA impaired the capacity of Wnt5a to: (1) induce recruitment of ARHGEF2 to ROR1, (2) enhance in vitro exchange activity of ARHGEF2 and (3) induce activation of RhoA and Rac1 in CLL cells. Furthermore, CRISPR/Cas9 deletion of 14-3-3ζ in ROR1-negative CLL cell-line MEC1, and in MEC1 cells transfected to express ROR1 (MEC1-ROR1), demonstrated that 14-3-3ζ was necessary for the growth/engraftment advantage of MEC1-ROR1 over MEC1 cells. We identified a binding motif (RSPS857SAS) in ROR1 for 14-3-3ζ. Site-directed mutagenesis of ROR1 demonstrated that serine-857 was required for the recruitment of 14-3-3ζ and ARHGEF2 to ROR1, and activation of RhoA and Rac1. Collectively, this study reveals that 14-3-3ζ plays a critical role in Wnt5a/ROR1 signaling, leading to enhanced CLL migration and proliferation
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