Impact of Triphala on kupffer Cell Regeneration : A Possible Mechanism

As we humans are far too aware, all mammals have a finite lifespan. Apart from basic longevity, age is also a major risk factor for most of the diseases. Since stem cells are responsible for maintaining tissue homeostasis throughout an organism’s lifetime, an attractive theory is that ageing-related phenotypes might be, at least in part, due to an alteration in the number and/or function of tissue stem cells. Here, we have focused on kupffer cell regeneration after ethanol cytotoxicity and possible role of cytokines and oxidative stress enzymes during the cascade along with genopreventive effect of Triphala in mouse bone marrow cell

Evaluación de la actividad diurética y laxante del extracto acuoso de hojas de Argemone mexicana en ratas

Objectives: Argemone mexicana have been widely studied for its several pharmacological benefits and has been used in traditional medicine to treat constipation like symptoms. The present study carried out to evaluate the extract for its diuretic and laxative potential. Method: The aqueous extract of Argemone mexicana prepared using percolation method and subjected to phytochemical analysis. Evaluation of diuretic and laxative activity was carried out using metabolic cage apparatus and flame photometer as per the standard method reported earlier. Frusemide (20 mg/kg) and sodium picosulate (5 mg/kg) were served as positive control for diuretic activity and laxative activity respectively. Result: The extract showed significant diuretic activity at 250 mg/kg dose when compared to standard frusemide. Even this extract also effective in increasing electrolyte concentration. Whereas the extract at 250 mg/kg showed significantly increasing in fecal output, and also significantly increased the weight of feces at 100 mg/kg and 200 mg/kg dose. Conclusion: The previous significant finding supports the traditional use of Argemone mexicana for its diuretic and laxative potential Objetivos: Argemone mexicana ha sido ampliamente estudiada por sus diversos beneficios farmacológicos y se ha utilizado en la medicina tradicional para tratar síntomas de estreñimiento. El presente estudio evaluó el extracto por su potencial diurético y laxante. Método: El extracto acuoso de Argemone mexicana se preparó utilizando el método de percolación y se sometió a análisis fitoquímico. La evaluación de la actividad diurética y laxante se llevó a cabo utilizando unas jaulas metabólicas y un fotómetro de llama según el método estándar descrito con anterioridad. Se administró frusemida (20 mg / kg) y picosulato de sodio (5 mg / kg) como control positivo de la actividad diurética y actividad laxante respectivamente. Resultado: El extracto mostró una actividad diurética significativa a una dosis de 250 mg / kg en comparación con la frusemida estándar. Incluso, este extracto también es efectivo para aumentar la concentración de electrolitos. Mientras que el extracto a 250 mg/kg mostró un aumento significativo en la producción fecal, y también aumentó significativamente el peso de las heces en ambas dosis. Conclusión: El hallazgo significativo anterior apoya el uso tradicional de Argemone mexicana por sus potencialidades diurética y laxante

Chromatographic estimation of maturity based phytochemical profiling of Ipomoea mauritiana

Collection of herbs at right maturity is one of such parameter which affect afficacy of medicinal plants. Standard reference markers used in quality control of herbal drugs mostly authenticate identity and not efficacy. In order to derive bioactive markers, knowledge regarding appropriate collection time for each herb is essential. Traditional medical knowledge is bioactivity-oriented and informs about best time of collection for certain medicinal species, as observed in case of Ipomoea mauritiana Jacq. (Vidari–Sanskrit). Only mature (bigger size) tubers of Ipomoea mauritiana are used by Traditional Medical Practitioners (TMP) for preparing galactagogues and immunomodulatory herbal medicines (Rasayan).Microscopy of transverse sections revealed structural variation between mature and immature tubers and girth of tubers determine the maturity of plant, the difference in phytochemical profiles of mature and immature tubers was observed.Variation in phytoconstituents of mature and immature tubers was confirmed through proximate analysis, phytochemical screening, qualitative HPLC and HPTLC analysis revealed the variation in phytoconstituents in mature and immature tubers.Keywords: Microscopy, HPLC, HPTLC, proximate analysis, phytochemical screening

Chromatographic estimation of maturity based phytochemical profiling of Ipomoea mauritiana

Collection of herbs at right maturity is one of such parameter which affect afficacy of medicinal plants. Standard reference markers used in quality control of herbal drugs mostly authenticate identity and not efficacy. In order to derive bioactive markers, knowledge regarding appropriate collection time for each herb is essential. Traditional medical knowledge is bioactivity-oriented and informs about best time of collection for certain medicinal species, as observed in case of Ipomoea mauritiana Jacq. (Vidari–Sanskrit). Only mature (bigger size) tubers of Ipomoea mauritiana are used by Traditional Medical Practitioners (TMP) for preparing galactagogues and immunomodulatory herbal medicines (Rasayan).Microscopy of transverse sections revealed structural variation between mature and immature tubers and girth of tubers determine the maturity of plant, the difference in phytochemical profiles of mature and immature tubers was observed.Variation in phytoconstituents of mature and immature tubers was confirmed through proximate analysis, phytochemical screening, qualitative HPLC and HPTLC analysis revealed the variation in phytoconstituents in mature and immature tubers.Keywords: Microscopy, HPLC, HPTLC, proximate analysis, phytochemical screening

Investigation of methylenetetrahydrofolate reductase C677T and factor V Leiden mutation as a genetic marker for retinal vein occlusion

PURPOSE: Thromboembolic phenomenon is one of the causes of retinal vein occlusion (RVO) which is in fact a multifactorial disease. Therefore, we aimed to study methylenetetrahydrofolate reductase gene polymorphism (MTHFR C677T) and factor V Leiden as genetic risk factors of RVO. MATERIALS AND METHODS: A total of 50 (19 males and 31 females) cases of RVO were compared with 50 age- and sex-matched (21 males and 29 females) controls. Complete ocular examination was done for all samples. The diagnosis of RVO was made clinically; however, fundus fluorescein angiography and optical coherence tomography were performed when needed. Serum homocysteine levels were estimated by automatic chemiluminescence analyzer, whereas MTHFR C677T and factor V Leiden mutations were detected by polymerase chain reaction–restriction fragment length polymorphism method. RESULTS: The mean age of RVO cases and controls was 54.62 ± 13.92 years and 58.72 ± 11.20 years, respectively. 48.3% of cases and 51.7% of controls were diabetic. 65.3% of cases were hypertensive proving hypertension as a strong risk factor (P = 0.003) of RVO. Serum homocysteine was also found significantly high (P = 0.025) with mean values of 19.98 ± 9.03 μmol/L and 16.98 ± 8.29 μmol/L in cases and controls, respectively. The MTHFR genotype (CT) was found in 83.3% patients of central RVO group and 78.6% cases of branch RVO group that was significantly associated with high serum homocysteine levels. Factor V Leiden mutation was absent in all individuals. CONCLUSION: Hyperhomocysteinemia is an important risk factor for RVO, especially in patients with MTHFR C677T gene polymorphism

Evaluación de la actividad diurética y laxante del extracto acuoso de Argenome mexicana en ratas

ABSTRACT Objectives: Argemone mexicana have been widely studied for its several pharmacological benefits and has been used in traditional medicine to treat constipation like symptoms. The present study carried out to evaluate the extract for its diuretic and laxative potential. Method: The aqueous extract of Argemone mexicana prepared using percolation method and subjected to phytochemical analysis. Evaluation of diuretic and laxative activity was carried out using metabolic cage apparatus and flame photometer as per the standard method reported earlier. Frusemide (20 mg/ kg) and sodium picosulate (5 mg/kg) were served as positive control for diuretic activity and laxative activity respectively. Result: The extract showed significant diuretic activity at 250 mg/kg dose when compared to standard frusemide. Even this extract also effective in increasing electrolyte concentration. Whereas the extract at 250 mg/kg showed significantly increasing in fecal output, and also significantly increased the weight of feces at 100 mg/kg and 200 mg/kg dose. Conclusion: The previous significant finding supports the traditional use of Argemone mexicana for its diuretic and laxative potentialRESUMEN Objetivos: Argemone mexicana ha sido ampliamente estudiada por sus diversos beneficios farmacológicos y se ha utilizado en la medicina tradicional para tratar síntomas de estreñimiento. El presente estudio evaluó el extracto por su potencial diurético y laxante. Método: El extracto acuoso de Argemone mexicana se preparó utilizando el método de percolación y se sometió a análisis fitoquímico. La evaluación de la actividad diurética y laxante se llevó a cabo utilizando unas jaulas metabólicas y un fotómetro de llama según el método estándar descrito con anterioridad. Se administró frusemida (20 mg / kg) y picosulato de sodio (5 mg / kg) como control positivo de la actividad diurética y actividad laxante respectivamente. Resultado: El extracto mostró una actividad diurética significativa a una dosis de 250 mg / kg en comparación con la frusemida estándar. Incluso, este extracto también es efectivo para aumentar la concentración de electrolitos. Mientras que el extracto a 250 mg/kg mostró un aumento significativo en la producción fecal, y también aumentó significativamente el peso de las heces en ambas dosis. Conclusión: El hallazgo significativo anterior apoya el uso tradicional de Argemone mexicana por sus potencialidades diurética y laxante