75 research outputs found
Polymorphisms in promoter sequences of MDM2, p53, and p16INK4a genes in normal Japanese individuals
Research has been conducted to identify sequence polymorphisms of gene promoter regions in patients and control subjects, including normal individuals, and to determine the influence of these polymorphisms on transcriptional regulation in cells that express wild-type or mutant p53. In this study we isolated genomic DNA from whole blood of healthy Japanese individuals and sequenced the promoter regions of the MDM2, p53, and p16INK4a genes. We identified polymorphisms comprising 3 nucleotide substitutions at exon 1 and intron 1 regions of the MDM2 gene and 1 nucleotide insertion at a poly(C) nucleotide position in the p53 gene. The Japanese individuals also exhibited p16INK4a polymorphisms at several positions, including position -191. Reporter gene analysis by using luciferase revealed that the polymorphisms of MDM2, p53, and p16INK4a differentially altered luciferase activities in several cell lines, including the Colo320DM, U251, and T98G cell lines expressing mutant p53. Our results indicate that the promoter sequences of these genes differ among normal Japanese individuals and that polymorphisms can alter gene transcription activity
<Abstract of published report>Studies on Inhibitors of Skins Tumor Promotion XI. : Inhibitory Effects of Flavonoids from Scutellaria baicalensis on Epstein-Barr Virus Activation and Their Anti-tumor-Promoting Activities.
Polymorphisms in promoter sequences of MDM2, p53, and p16INK4a genes in normal Japanese individuals
Enhancement of Carotenoid Concentration in the Flesh of Japanese Persimmon ‘Fuyu’ by High Postharvest Temperature Treatments
Cypellocarpins A−C, Phenol Glycosides Esterified with Oleuropeic Acid, from Eucalyptus cypellocarpa
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