10 research outputs found
Sexual Dimorphism and Reproductive Status of the Red Swamp Crayfish Procambarus clarkii
The red swamp crayfish Procambarus clarkii is native to northeastern Mexico and the south-central USA, and it has expanded its distribution worldwide and negatively impacted the ecosystems in the invaded regions. The dynamics of the P. clarkii populations have been studied as the basis for the development of effective control measures against this invasive alien species. Adult males of P. clarkii exhibit a cyclical dimorphism between two sexual morphotypes; reproductive form I has large chelae and non-reproductive form II has small chelae. However, whether P. clarkii females have two sexual morphotypes and exhibit form alternation has not been resolved, and little is known about the degree of intra-sexual dimorphism of the chelae, even among males. We employed allometric growth analysis on the chelae dimensions of P. clarkii females and males that were collected from a small pond in Yokohama, Japan. Our analysis demonstrated the existence of form I, which has larger chelae, and form II, which has smaller chelae, in P. clarkii females and highlighted the intra- and inter-sexual dimorphisms in the chelae of this species. The reproductive cycle of the population was successfully traced by the reproductive status of P. clarkii based on the occurrence patterns of each sexual morphotype; the form I crayfish occurred throughout the sampling period from April to December, while the occurrences of form I females and males were highly correlated, peaking in October. Our results suggested that alternation of sexual forms occurs in P. clarkii females. The ability to discriminate between the sexual morphotypes based on chelae allometric growth would allow us to evaluate the female reproductive status more easily ansd precisely in invasive P. clarkii populations
Larval Performance of Amphidromous and Landlocked Atyid Shrimp Species in the Genus Paratya under Different Feeding Conditions
Freshwater shrimps in the family Atyidae exhibit two life history traits: 1) amphidromy, with many small embryos hatching into planktotrophic larvae that develop in the sea, and 2) landlocked, with few large embryos hatching into non-feeding lecithotrophic larvae, or facultative lecithotrophic larvae that develop in freshwater. The lecithotrophy of larvae is considered an adaptation to limited food conditions in the freshwater environment. Furthermore, faster development and settlement behaviours that large larvae exhibit are considered adaptations that allow the larvae to stay in or near parental habitats in fast-flowing streams. We therefore hypothesized that the facultative lecithotrophic larvae of landlocked shrimps might better adapt to limited food conditions as their large body size develops, an adaptation to maintain habitat position in flowing streams, than do planktotrophic larvae of companion amphidromous species developing in the sea. To test this hypothesis, we compared the larval feeding habits and size of two closely related species in the genus Paratya: the amphidromous P. compressa, with planktotrophic larvae, and the landlocked P. improvisa, with facultative lecithotrophic larvae. Larvae were reared by being fed commercially preserved or cultured phytoplankton (Tetraselmis sp.) and cultured zooplankton rotifers. Paratya compressa larvae did not survive, but P. improvisa larvae did develop into the juvenile stage under poor feeding conditions with preserved Tetraselmis alone, supporting our hypothesis of the effects of different larval feeding habits in amphidromous and landlocked atyid shrimp species. Hatchlings were larger and larval duration was shorter in P. improvisa than in P. compressa. Paratya improvisa larvae exhibited settlement behaviour beginning with the early zoeal stage. Our results also highlighted the retention strategy by which landlocked P. improvisa larvae stay in or near parental habitats
Larval Performance of Amphidromous and Landlocked Atyid Shrimp Species in the Genus Paratya under Different Feeding Conditions
Freshwater shrimps in the family Atyidae exhibit two life history traits: 1) amphidromy, with many small embryos hatching into planktotrophic larvae that develop in the sea, and 2) landlocked, with few large embryos hatching into non-feeding lecithotrophic larvae, or facultative lecithotrophic larvae that develop in freshwater. The lecithotrophy of larvae is considered an adaptation to limited food conditions in the freshwater environment. Furthermore, faster development and settlement behaviours that large larvae exhibit are considered adaptations that allow the larvae to stay in or near parental habitats in fast-flowing streams. We therefore hypothesized that the facultative lecithotrophic larvae of landlocked shrimps might better adapt to limited food conditions as their large body size develops, an adaptation to maintain habitat position in flowing streams, than do planktotrophic larvae of companion amphidromous species developing in the sea. To test this hypothesis, we compared the larval feeding habits and size of two closely related species in the genus Paratya: the amphidromous P. compressa, with planktotrophic larvae, and the landlocked P. improvisa, with facultative lecithotrophic larvae. Larvae were reared by being fed commercially preserved or cultured phytoplankton (Tetraselmis sp.) and cultured zooplankton rotifers. Paratya compressa larvae did not survive, but P. improvisa larvae did develop into the juvenile stage under poor feeding conditions with preserved Tetraselmis alone, supporting our hypothesis of the effects of different larval feeding habits in amphidromous and landlocked atyid shrimp species. Hatchlings were larger and larval duration was shorter in P. improvisa than in P. compressa. Paratya improvisa larvae exhibited settlement behaviour beginning with the early zoeal stage. Our results also highlighted the retention strategy by which landlocked P. improvisa larvae stay in or near parental habitat
Functional reassignment of Cellvibrio vulgaris EpiA to cellobiose 2-epimerase and an evaluation of the biochemical functions of the 4-O-beta-d-mannosyl-d-glucose phosphorylase-like protein, UnkA
The aerobic soil bacterium Cellvibrio vulgaris has a beta-mannan-degradation gene cluster, including unkA, epiA, man5A, and aga27A. Among these genes, epiA has been assigned to encode an epimerase for converting d-mannose to d-glucose, even though the amino acid sequence of EpiA is similar to that of cellobiose 2-epimerases (CEs). UnkA, whose function currently remains unknown, shows a high sequence identity to 4-O-beta-d-mannosyl-d-glucose phosphorylase. In this study, we have investigated CE activity of EpiA and the general characteristics of UnkA using recombinant proteins from Escherichia coli. Recombinant EpiA catalyzed the epimerization of the 2-OH group of sugar residue at the reducing end of cellobiose, lactose, and beta-(1 -> 4)-mannobiose in a similar manner to other CEs. Furthermore, the reaction efficiency of EpiA for beta-(1 -> 4)-mannobiose was 5.5x10(4)-fold higher than it was for d-mannose. Recombinant UnkA phosphorolyzed beta-d-mannosyl-(1 -> 4)-d-glucose and specifically utilized d-glucose as an acceptor in the reverse reaction, which indicated that UnkA is a typical 4-O-beta-d-mannosyl-d-glucose phosphorylase
Functional reassignment of Cellvibrio vulgaris EpiA to cellobiose 2-epimerase and an evaluation of the biochemical functions of the 4-O-beta-d-mannosyl-d-glucose phosphorylase-like protein, UnkA
Functional reassignment of Cellvibrio vulgaris
The aerobic soil bacterium Cellvibrio vulgaris has a beta-mannan-degradation gene cluster, including unkA, epiA, man5A, and aga27A. Among these genes, epiA has been assigned to encode an epimerase for converting d-mannose to d-glucose, even though the amino acid sequence of EpiA is similar to that of cellobiose 2-epimerases (CEs). UnkA, whose function currently remains unknown, shows a high sequence identity to 4-O-beta-d-mannosyl-d-glucose phosphorylase. In this study, we have investigated CE activity of EpiA and the general characteristics of UnkA using recombinant proteins from Escherichia coli. Recombinant EpiA catalyzed the epimerization of the 2-OH group of sugar residue at the reducing end of cellobiose, lactose, and beta-(1 -> 4)-mannobiose in a similar manner to other CEs. Furthermore, the reaction efficiency of EpiA for beta-(1 -> 4)-mannobiose was 5.5x10(4)-fold higher than it was for d-mannose. Recombinant UnkA phosphorolyzed beta-d-mannosyl-(1 -> 4)-d-glucose and specifically utilized d-glucose as an acceptor in the reverse reaction, which indicated that UnkA is a typical 4-O-beta-d-mannosyl-d-glucose phosphorylase
Osteoid osteoma of the acetabulum successfully treated with computed tomography-guided resection and ablation using a standard electrosurgical generator: a case report
IL-6 blockade in the management of non-infectious uveitis
Several pathogenetic studies have paved the way for a newer more rational therapeutic approach to non-infectious uveitis, and treatment of different forms of immune-driven uveitis has drastically evolved in recent years after the advent of biotechnological drugs. Tumor necrosis factor-α targeted therapies, the first-line recommended biologics in uveitis, have certainly led to remarkable results in patients with non-infectious uveitis. Nevertheless, the decision-making process turns out to be extremely difficult in anti-tumor necrosis factor or multidrug-resistant cases. Interleukin (IL)-6 holds a critical role in the pathogenic pathways of uveitis, due to its extended and protean range of effects. On this background, manipulation of IL-6 inflammatory cascade has unraveled encouraging outcomes. For instance, rising evidence has been achieved regarding the successful use of tocilizumab, the humanized monoclonal antibody targeted against the IL-6 receptor, in treating uveitis related to juvenile idiopathic arthritis or Behçet’s disease. Similar findings have also been reported for uveitis associated with systemic disorders, such as rheumatoid arthritis or multicentric Castleman disease, but also for idiopathic uveitis, the rare birdshot chorioretinopathy, and even in cases complicated by macular edema. This work provides a digest of all current experiences and evidences concerning IL-6 blockade, as suggested by the medical literature, proving its potential role in the management of non-infectious uveitis