Expression of VP60 gene from rabbit haemorrhagic disease virus (RHDV) YL strain under control of the ats1A promoter in tobacco

The VP60 gene from rabbit haemorrhagic disease virus (RHDV) YL strain in Northeast of China, under control of the ats1A promoter from Rubisco small subunit genes of Arabidopsis thaliana, was introduced into the transfer deoxyribonucleic acid (T-DNA) region of plant transfer vector pCAMBIA1300 and transferred to tobacco (Nicotiana tabacum cv. Petit Havanna SR1) with Agrobacterium tumefaciens-mediated method. Polymerase chain reaction (PCR) reverse transcription(RT) and -PCR analysis of the transformed tobacco plants confirmed the integration of the VP60 gene copy into the plant DNA and VP60 gene transcription produced. Western blot analysis revealed that the VP60 protein was expressed in tobacco under control of ats1A promoter.Key words: Agrobacterium tumefaciens, rabbit haemorrhagic disease virus (RHDV), VP60 protein, ats1A promoter, plant-derived vaccine

Low-energy structures of clusters supported on metal fcc(110) surfaces

The low-energy structures (LESs) of adatom clusters on a series of metal face-centered cubic (fcc) (110) surfaces are systematically studied by the genetic algorithm, and a simplified model based on the atomic interactions is developed to explain the LESs. Two different kinds of LES group mainly caused by the different next nearest-neighbor (NNN) adatom-adatom interaction are distinguished, although the NNN atomic interaction is much weaker than the nearest-neighbor interaction. For a repulsive NNN atomic interaction, only the linear chain is included in the LES group. However, for an attractive one, type of structure in the LES group is various and replace gradually one by one with cluster size increasing. Based on our model, we also predict the shape feature of the large cluster which is found to be related closely to the ratio of NN and NNN bond energies, and discuss the surface reconstruction in the view of atomic interaction. The results are in accordance with the experimental observations

Approximate Path Searching Method for Single-Satellite Observation and Transmission Task Planning Problem

Satellite task planning not only plans the observation tasks to collect images of the earth surface, but also schedules the transmission tasks to download images to the ground station for users’ using, which plays an important role in improving the efficiency of the satellite observation system. However, most of the work to our knowledge, scheduling the observation and transmission tasks separately, ignores the correlation between them in resource (e.g., energy and memory) consumption and acquisition. In this paper, we study the single-satellite observation and transmission task planning problem under a more accurate resource usage model. Two preprocessing strategies including graph partition and nondominated subpaths selection are used to decompose the problem, and an improved label-setting algorithm with the lower bound cutting strategy is proposed to maximize the total benefit. Finally, we compare the proposed method with other three algorithms based on three data sets, and the experimental result shows that our method can find the near-optimal solution in much less time

Optimisation of the Hydrodynamic Performance of a wave energy converter in an Integrated Cylindrical WEC-Type Breakwater System

Wave energy converters (WECs) are built to extract wave energy. However, this kind of device is still expensive for commercial utilization. To cut down the cost of WECs by sharing the construction cost with breakwaters, an integrated cylindrical WEC-type breakwater system that includes a cylindrical WEC array in front of a very long breakwater is proposed to extract wave energy and attenuate incident waves. This paper aims to optimize the performance of the integrated cylindrical WEC-type breakwater system. A computational fluid dynamics tool, openfoam®, and a potential flow theory-based solver, HAMS®, are utilized. openfoam® provides viscosity corrections to a modified version of HAMS® in order to accurately and efficiently predict the integrated system’s performance. Parametric studies are conducted to optimize the integrated system, and a novel setup with an extra arc structure is found to significantly improve the performance of the integrated system