PERCEIVED CHALLENGES AND SATISFACTION OF EDUCATION STUDENTS IN ONLINE DISTANCE LEARNING

The study aimed to determine the perceived challenges and satisfaction levels of Bachelor of Elementary Education in online distance learning. The descriptive-correlational design was used in this study. The investigations were conducted at Notre Dame of Midsayap College on fifty (50) BEED students who are enrolled in online distance learning for School Year 2021-2022 and the majority of them were female students. A researcher-modified survey questionnaire was used in data gathering, Frequency and percentage distribution, mean and standard deviation, t-test and ANOVA, and Pearson-r correlation were used in treating the data. The study revealed that the students have challenges in online distance learning. The study found out that the highest challenges encountered by the students in online distance learning are slow internet connection due to their location and facing many interruptions when learning at home such as household chores and other errands. The students are highly satisfied with how teachers showed interest in helping students to learn and the teacher’s accessibility and availability. Moreover, the respondents are least satisfied with how timely teachers give feedback on their performance. The study also revealed that there was a significant difference in the satisfaction level of the students in terms of their sex.  Article visualizations

CLEAR: Spatially Resolved Emission Lines and Active Galactic Nuclei at 0.6<z<1.30.6<z<1.3

We investigate spatially-resolved emission-line ratios in a sample of 219 galaxies ($0.6<z<1.3$) detected using the G102 grism on the \emph{Hubble Space Telescope} Wide Field Camera 3, taken as part of the CANDELS Ly$\alpha$ Emission at Reionization (CLEAR) survey, to measure ionization profiles and search for low-luminosity active galactic nuclei (AGN). We analyze \OIII\ and \Hb\ emission-line maps, enabling us to spatially resolve the \OIIIHb\ emission-line ratio across the galaxies in the sample. We compare the \OIIIHb\ ratio in galaxy centers and outer annular regions to measure ionization gradients and investigate the potential of sources with nuclear ionization to host AGN. We investigate some of the individual galaxies that are candidates to host strong nuclear ionization and find that they often have low stellar mass and are undetected in X-rays, as expected for low-luminosity AGN in low-mass galaxies. We do not find evidence for a significant population of off-nuclear AGN or other clumps of off-nuclear ionization. We model the observed distribution of \OIIIHb\ gradients and find that most galaxies are consistent with small or zero gradients, but 6-16\% of galaxies in the sample are likely to host nuclear \OIIIHb\ that is $\sim$0.5~dex higher than in their outer regions. This study is limited by large uncertainties in most of the measured \OIIIHb\ spatial profiles, therefore deeper data, e.g, from deeper \textit{HST}/WFC3 programs or from \textit{JWST}/NIRISS, are needed to more reliably measure the spatially resolved emission-line conditions of individual high-redshift galaxies.Comment: 16 pages, 13 figures, 2 table

Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos

© The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Naert, T., Tulkens, D., Edwards, N. A., Carron, M., Shaidani, N. I., Wlizla, M., Boel, A., Demuynck, S., Horb, M. E., Coucke, P., Willaert, A., Zorn, A. M., & Vleminckx, K. Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos. Scientific Reports, 10(1), (2020): 14662, doi:10.1038/s41598-020-71412-0.CRISPR/Cas9 genome editing has revolutionized functional genomics in vertebrates. However, CRISPR/Cas9 edited F0 animals too often demonstrate variable phenotypic penetrance due to the mosaic nature of editing outcomes after double strand break (DSB) repair. Even with high efficiency levels of genome editing, phenotypes may be obscured by proportional presence of in-frame mutations that still produce functional protein. Recently, studies in cell culture systems have shown that the nature of CRISPR/Cas9-mediated mutations can be dependent on local sequence context and can be predicted by computational methods. Here, we demonstrate that similar approaches can be used to forecast CRISPR/Cas9 gene editing outcomes in Xenopus tropicalis, Xenopus laevis, and zebrafish. We show that a publicly available neural network previously trained in mouse embryonic stem cell cultures (InDelphi-mESC) is able to accurately predict CRISPR/Cas9 gene editing outcomes in early vertebrate embryos. Our observations can have direct implications for experiment design, allowing the selection of guide RNAs with predicted repair outcome signatures enriched towards frameshift mutations, allowing maximization of CRISPR/Cas9 phenotype penetrance in the F0 generation.Research in the Vleminckx laboratory is supported by the Research Foundation—Flanders (FWO-Vlaanderen) (Grants G0A1515N and G029413N), by the Belgian Science Policy (Interuniversity Attraction Poles—IAP7/07) and by the Concerted Research Actions from Ghent University (BOF15/GOA/011). Further support was obtained by the Hercules Foundation, Flanders (Grant AUGE/11/14) and the Desmoid Tumor Research Foundation and the Desmoid Tumour Foundation Canada. T.N. is funded by “Kom op tegen Kanker” (Stand up to Cancer), the Flemish cancer society and previously held PhD fellowship with VLAIO-HERMES during the course of this work. D.T. and M. C. hold a PhD fellowship from the Research Foundation-Flanders (FWO-Vlaanderen). The Zorn Lab is supported by Funding from NIH National Institute of Child Health and Human Development (NICHD) P01 HD093363. A.W. and A.B. are supported by the Ghent University (Universiteit Gent) Methusalem grant BOFMET2015000401 to Anne De Paepe. The National Xenopus Resource and Horb lab is supported by funding from the National Institutes of Health (P40 OD010997 and R01 HD084409)

CLEAR: The Gas-Phase Metallicity Gradients of Star-Forming Galaxies at 0.6 < z < 2.6

We report on the gas-phase metallicity gradients of a sample of 264 star-forming galaxies at 0.6 < z < 2.6, measured through deep near-infrared Hubble Space Telescope slitless spectroscopy. The observations include 12-orbit depth Hubble/WFC3 G102 grism spectra taken as a part of the CANDELS Lya Emission at Reionization (CLEAR) survey, and archival WFC3 G102+G141 grism spectra overlapping the CLEAR footprint. The majority of galaxies (84%) in this sample are consistent with a zero or slightly positive metallicity gradient across the full mass range probed (8.5 < log M_*/M_sun < 10.5). We measure the intrinsic population scatter of the metallicity gradients, and show that it increases with decreasing stellar mass---consistent with previous reports in the literature, but confirmed here with a much larger sample. To understand the physical mechanisms governing this scatter, we search for correlations between the observed gradient and various stellar population properties at fixed mass. However, we find no evidence for a correlation with the galaxy properties we consider---including star-formation rates, sizes, star-formation rate surface densities, and star-formation rates per gravitational potential energy. We use the observed weakness of these correlations to provide material constraints for predicted intrinsic correlations from theoretical models.Comment: 19 pages, 10 figures (v2: typo fixed in Figure 10 label); submitted to Ap

CLEAR: Paschen-β\beta Star Formation Rates and Dust Attenuation of Low Redshift Galaxies

We use \Pab\ (1282~nm) observations from the Hubble Space Telescope (\HST) G141 grism to study the star-formation and dust attenuation properties of a sample of 29 low-redshift ($z < 0.287$) galaxies in the CANDELS Ly$\alpha$ Emission at Reionization (CLEAR) survey. We first compare the nebular attenuation from \Pab/\Ha with the stellar attenuation inferred from the spectral energy distribution, finding that the galaxies in our sample are consistent with an average ratio of the continuum attenuation to the nebular gas of 0.44, but with a large amount of excess scatter beyond the observational uncertainties. Much of this scatter is linked to a large variation between the nebular dust attenuation as measured by (space-based) \Pab to (ground-based) \Ha to that from (ground-based) \Ha/\Hb. This implies there are important differences between attenuation measured from grism-based / wide-aperture \Pab fluxes and the ground-based / slit-measured Balmer decrement. We next compare star-formation rates (SFRs) from \Pab to those from dust-corrected UV. We perform a survival analysis to infer a census of \Pab\ emission implied by both detections and non-detections. We find evidence that galaxies with lower stellar mass have more scatter in their ratio of \Pab\ to attenuation-corrected UV SFRs. When considering our \Pab\ detection limits, this observation supports the idea that lower mass galaxies experience "burstier" star-formation histories. Together, these results show that \Pab\ is a valuable tracer of a galaxy's SFR, probing different timescales of star-formation and potentially revealing star-formation that is otherwise missed by UV and optical tracers.Comment: 19 pages, 14 figures, 2 table

CLEAR: High-Ionization [Ne V] λ{\lambda}3426 Emission-line Galaxies at 1.4<z<2.31.4 <z< 2.3

We analyze a sample of 25 [Ne V] $\lambda$3426 emission-line galaxies at $1.4<z<2.3$ using Hubble Space Telescope/Wide Field Camera 3 G102 and G141 grism observations from the CANDELS Lyman-$\alpha$ Emission at Reionization (CLEAR) survey. [Ne V] emission probes extremely energetic photoionization (97.11-126.21 eV), and is often attributed to energetic radiation from active galactic nuclei (AGN), shocks from supernova, or an otherwise very hard ionizing spectrum from the stellar continuum. In this work, we use [Ne V] in conjunction with other rest-frame UV/optical emission lines ([O II] $\lambda\lambda$3726,3729, [Ne III] $\lambda$3869, H$\beta$, [O III] $\lambda\lambda$4959,5007, H$\alpha$+[N II] $\lambda\lambda$6548,6583, [S II] $\lambda\lambda$6716,6731), deep (2--7 Ms) X-ray observations (from Chandra), and mid-infrared imaging (from Spitzer) to study the origin of this emission and to place constraints on the nature of the ionizing engine. The majority of the [Ne V]-detected galaxies have properties consistent with ionization from AGN. However, for our [Ne V]-selected sample, the X-ray luminosities are consistent with local ($z\lesssim 0.1$) X-ray-selected Seyferts, but the [Ne V] luminosities are more consistent with those from $z\sim 1$ X-ray-selected QSOs. The excess [Ne V] emission requires either reduced hard X-rays, or a $\sim$0.1 keV excess. We discuss possible origins of the apparent [Ne V] excess, which could be related to the ``soft (X-ray) excess'' observed in some QSOs and Seyferts, and/or be a consequence of a complex/anisotropic geometry for the narrow line region, combined with absorption from a warm, relativistic wind ejected from the accretion disk. We also consider implications for future studies of extreme high-ionization systems in the epoch of reionization ($z \gtrsim 6$) with JWST.Comment: 17 pages + 5 (appendix), 7 figures + 2(appendix

Using [Ne V]/[Ne III] to Understand the Nature of Extreme-Ionization Galaxies

Spectroscopic studies of extreme-ionization galaxies (EIGs) are critical to our understanding of exotic systems throughout cosmic time. These EIGs exhibit spectral features requiring >54.42 eV photons: the energy needed to fully ionize helium into He2+ and emit He II recombination lines. They are likely key contributors to reionization, and they can also probe exotic stellar populations or accretion onto massive black holes. To facilitate the use of EIGs as probes of high ionization, we focus on ratios constructed from strong rest-frame UV/optical emission lines, specifically [O III] 5008, H-beta, [Ne III] 3870, [O II] 3727,3729, and [Ne V] 3427. These lines probe the relative intensity at energies of 35.12, 13.62, 40.96, 13.62 eV, and 97.12, respectively, covering a wider range of ionization than traced by other common rest-frame UV/optical techniques. We use ratios of these lines ([Ne V]/[Ne III] = Ne53 and [Ne III]/[O II]), which are closely separated in wavelength, and mitigates effects of dust attenuation and uncertainties in flux calibration. We make predictions from photoionization models constructed from Cloudy that use a broad range of stellar populations and black hole accretion models to explore the sensitivity of these line ratios to changes in the ionizing spectrum. We compare our models to observations from the Hubble Space Telescope and James Webb Space Telescope of galaxies with strong high-ionization emission lines at z ~ 0, z ~ 2, and z ~ 7. We show that the Ne53 ratio can separate galaxies with ionization from 'normal' stellar populations from those with AGN and even 'exotic' Population III models. We introduce new selection methods to identify galaxies with photoionization driven by Population III stars or intermediate-mass black hole accretion disks that could be identified in upcoming high-redshift spectroscopic surveys.Comment: 16 pages, 5 figures, 1 table. Accepted in Ap

α-Arrestins Aly1 and Aly2 Regulate Intracellular Trafficking in Response to Nutrient Signaling

Arrestins, known regulators of endocytosis, take on novel functions in nutrient-regulated endosomal recycling. Yeast α-arrestins, Aly1 and Aly2, redistribute the Gap1 permease from endosomes to the cell surface and interact with clathrin/AP-1. Aly2 is regulated by the Npr1 kinase and acts through mechanisms distinct from Aly1

NGDEEP Epoch 1: Spatially Resolved Hα\alpha Observations of Disk and Bulge Growth in Star-Forming Galaxies at z∼z \sim 0.6-2.2 from JWST NIRISS Slitless Spectroscopy

We study the H$\alpha$ equivalent width, EW(H$\alpha$), maps of 19 galaxies at $0.6 < z < 2.2$ in the Hubble Ultra Deep Field (HUDF) derived from NIRISS slitless spectroscopy as part of the Next Generation Deep Extragalactic Exploratory Public (NGDEEP) Survey. Our galaxies mostly lie on the star-formation main sequence with a stellar mass range of $\mathrm{10^9 - 10^{11} M_\odot}$, and are therefore characteristic of "typical" star-forming galaxies at these redshifts. Leveraging deep HST and JWST broad-band images, spanning 0.4-4 $\mu$m, we perform spatially-resolved fitting of the spectral energy distributions (SEDs) for these galaxies and construct specific star formation rate (sSFR) and stellar-mass-weighted age maps. We compare these to the EW(H$\alpha$) maps with a spatial resolution of $\sim$1 kpc. The pixel-to-pixel EW(H$\alpha$) increases with increasing sSFR and with decreasing age, with the average trend slightly different from the relations derived from integrated fluxes of galaxies from the literature. Quantifying the radial profiles of EW(H$\alpha$), sSFR, and age, the majority (84%) of galaxies show positive EW(H$\alpha$) gradients, positive sSFR gradients, and negative age gradients, in line with the the inside-out quenching scenario. A few galaxies (16%) show inverse (and flat) trends possibly due to merging or starbursts. Comparing the distributions of EW(H$\alpha$) and sSFR to the star formation history models as a function of galactocentric radius, the central region of galaxies (e.g., their bulges) have experienced, at least one, rapid star-formation episodes, which leads to the formation of bulge, while their outer regions (e.g., disks) grow in a more steady-state. These results demonstrate the ability to study resolved star formation in distant galaxies with JWST NIRISS.Comment: 22 pages, 11 figure

Systematic Mutational Analysis of the Intracellular Regions of Yeast Gap1 Permease

The yeast general amino acid permease Gap1 is a convenient model for studying the intracellular trafficking of membrane proteins. Present at the plasma membrane when the nitrogen source is poor, it undergoes ubiquitin-dependent endocytosis and degradation upon addition of a good nitrogen source, e.g. ammonium. It comprises 12 transmembrane domains (TM) flanked by cytosol-facing N- and C-terminal tails (NT, CT). The NT of Gap1 contains the acceptor lysines for ubiquitylation and its CT includes a sequence essential to exit from the endoplasmic reticulum (ER).Journal ArticleResearch Support, Non-U.S. Gov'tSCOPUS: ar.jinfo:eu-repo/semantics/publishe