A New Cryptic Species of the Genus Mychonastes (Chlorophyceae, Sphaeropleales)

A new species of green coccoid algae, Mychonastes hindakii sp. nov., was isolated from the River Moscow (Russia, Moscow). The taxon is described using morphological and molecular methods. Mychonastes hindakii sp. nov. belongs to the group of species of the genus Mychonastes with spherical single cells joined with mucilaginous, irregularly shaped stalks. A comparison of ITS2 rDNA sequences and its secondary structures combined with the compensatory base changes approach confirms the separation between Mychonastes hindakii and other species of the genus. Mychonastes hindakii sp. nov. represents a cryptic species that can only be reliably identified using molecular data

Gusev, E.; Martynenko, N. Description of two new species of the genus Mallomonas from section Mallomonas (Synurales, Chrysophyceae) in the tropics. TABLE S1

This dataset comtains basic characteristics of localities and  distribution of M. pseudoelongata and M. pseudocorymbosa  in Viet Nam.</p

Martynenko et al., 2022 Cryptomonas terrestris Table S1

This table contains names of species, strains, their SSU, LSU+ITS2 rDNA and psbA cpDNA  Accession Numbers in GenBank, and also country, habitat and source of their origin.</p

Silica-Scaled Chrysophytes of Teletskoye Lake and Adjacent Area with a Description of a New Species from the Genus <i>Mallomonas</i>

The silica-scaled chrysophyte flora of some lakes and rivers of the Altai Mountains was investigated by means of electron microscopy. Teletskoye Lake; the Biya, Chulyshman, and Katun Rivers; and small lakes around the area were studied. A total of sixteen taxa were recorded, including eight belonging to Mallomonas, five to Synura, and three to Paraphysomonas. A revision of previously reported taxa of scaled chrysophytes in this region was carried out. One species new to science from the genus Mallomonas was found and described. Mallomonas altaica sp. nov. belongs to the section Striatae and is characterised by scales with ribs covering only the anterior part of the shield and a posterior flange almost completely covered with the secondary siliceous layer. This species was discovered only at Teletskoye Lake

Diversity of Silica-Scaled Chrysophytes in Central Vietnam

This paper focuses on the flora of scale-bearing chrysophytes from eight provinces located in the central part of Vietnam. Khanh Hoa, Phu Yen, Binh Dinh, Thua Thien Hue, Quang Tri, and Quang Binh provinces are located in the coastal area of Vietnam. Lam Dong and Dak Lak provinces represent mountain territories with an elevation of 500–2000 metres above sea level. In total, 212 water bodies of different origins were studied. Samples were obtained from swamp areas, lakes, rivers, reservoirs, ponds, and small temporary water bodies. In total, 76 taxa were identified by electron microscopic observations of samples. A total of 54 taxa were found in the mountainous provinces, while 73 were found in the coastal provinces. Of these, 51 species are common for both areas. The most diverse was the genus Mallomonas with 66 species, varieties, and forms; followed by Synura with 7 taxa; Chrysosphaerella with 2; and Spiniferomonas with 1. Seven taxa of the genus Mallomonas were not identified to the lower rank. All these unidentified specimens may potentially represent new species for science. Ten taxa are reported for the first time in Vietnam

Description of a New Species of the Genus Cryptomonas (Cryptophyceae: Cryptomonadales), Isolated from Soils in a Tropical Forest

A new species, Cryptomonas tropica sp. nov., is described from Cat Tien National Park (Vietnam) based on morphological and molecular data. Strains of the new species were isolated from soil, which is an unusual environment for photosynthetic cryptomonads. This species has elliptical cells in ventral view and a single plastid notched into several irregular lobes without microscopically visible pyrenoids. Phylogenetic relationships inferred from nuclear-encoded SSU, LSU, ITS2 rDNA and psbA cpDNA show that the new species forms an independent branch on the phylogenetic tree of the genus Cryptomonas. In all phylogenetic analyses, this lineage was sister to clades containing other small-celled, pyrenoid-less species: Cryptomonas erosa, C. parmana, C. macilenta, C. obovoidea and C. commutata. C. tropica has been observed in two distant localities in Cat Tien National Park

Description of a New Species of the Genus <i>Cryptomonas</i> (Cryptophyceae: Cryptomonadales), Isolated from Soils in a Tropical Forest

A new species, Cryptomonas tropica sp. nov., is described from Cat Tien National Park (Vietnam) based on morphological and molecular data. Strains of the new species were isolated from soil, which is an unusual environment for photosynthetic cryptomonads. This species has elliptical cells in ventral view and a single plastid notched into several irregular lobes without microscopically visible pyrenoids. Phylogenetic relationships inferred from nuclear-encoded SSU, LSU, ITS2 rDNA and psbA cpDNA show that the new species forms an independent branch on the phylogenetic tree of the genus Cryptomonas. In all phylogenetic analyses, this lineage was sister to clades containing other small-celled, pyrenoid-less species: Cryptomonas erosa, C. parmana, C. macilenta, C. obovoidea and C. commutata. C. tropica has been observed in two distant localities in Cat Tien National Park

A New Cryptic Species of the Genus <i>Mychonastes</i> (Chlorophyceae, Sphaeropleales)

A new species of green coccoid algae, Mychonastes hindakii sp. nov., was isolated from the River Moscow (Russia, Moscow). The taxon is described using morphological and molecular methods. Mychonastes hindakii sp. nov. belongs to the group of species of the genus Mychonastes with spherical single cells joined with mucilaginous, irregularly shaped stalks. A comparison of ITS2 rDNA sequences and its secondary structures combined with the compensatory base changes approach confirms the separation between Mychonastes hindakii and other species of the genus. Mychonastes hindakii sp. nov. represents a cryptic species that can only be reliably identified using molecular data

Phylogenetic relationships and status of taxa of Pulsatilla uralensis and P. patens s.str. (Ranunculaceae) in north-eastern European Russia

We studied the allopatric complex Pulsatilla patens (L.) Mill. s.lat. (Ranunculaceae) in north-eastern European Russia and the Urals. In this region, there are two kinds of P. patens with different perianth colours in monochrome and polychrome populations. To clarify their taxonomic boundaries, we used the sequences of chloroplast DNA (rbcL and matK) and nuclear DNA (ITS2), in addition to morphological characteristics. The combination of three markers (rbcL+matK+ITS2) was found to be the most effective for phylogenetic resolution. The samples of two morphologically-different taxa P. uralensis and P. patents s.str. were shown to form a single clade on the phylogenetic tree. Based on the molecular phylogenetic analysis, we were not able to unequivocally prove the independent existence of P. uralensis

Phylogenetic relationships and status of taxa of Pulsatilla uralensis and P. patens s.str. (Ranunculaceae) in north-eastern European Russia

We studied the allopatric complex Pulsatilla patens (L.) Mill. s.lat. (Ranunculaceae) in north-eastern European Russia and the Urals. In this region, there are two kinds of P. patens with different perianth colours in monochrome and polychrome populations. To clarify their taxonomic boundaries, we used the sequences of chloroplast DNA (rbcL and matK) and nuclear DNA (ITS2), in addition to morphological characteristics. The combination of three markers (rbcL+matK+ITS2) was found to be the most effective for phylogenetic resolution. The samples of two morphologically-different taxa P. uralensis and P. patents s.str. were shown to form a single clade on the phylogenetic tree. Based on the molecular phylogenetic analysis, we were not able to unequivocally prove the independent existence of P. uralensis