Corporate Culture and Empathy and Excitement Labor of Service Employees inService Company, Mainly at Tokyo Disneyland

東京ディズニーランド(ディズニーシーを含むパーク)の大成功(集客と驚異的リピート率)の要因は、①「夢と魔法の王国」にふさわしいアトラクション1、②接客従業員(主に、非正社員、キャラクターを含む)のホスピタリティ・サービスが、顧客に「素晴らしい思い出に残る感動経験」を与えていることである。望ましいサービス労働のあり方は「顧客・従業員インターラクティブの共感に基づく従業員の感動労働」であるという仮説をたて、その解明を研究目的とした。①先行研究の考察、②運営会社へのインタビュー、③現場でのキャストのサービス労働の実査と簡単な質問、④顧客へのヒストリカル・インタビュー・アンケート実施という研究方法によって、接客従業員の「共感・感動労働」を実証中である。共感・感動労働の視点で、東京ディズニーランドと日本マクドナルド、スターバックスコーヒーとを比較した

Dasatinib promotes nuclear shuttling of ER in the MCF7-TAMR cells but not the 1%MCF7.

<p>1%MCF7 and MCF7-TAMR cells were treated for 24-hours with 1%FBS, dasatinib (100nM), 4-OHT (10nM), or the combination and stained with ER, pSrc^tyr416 and DAPI; bars indicate 20μm <b>(A)</b>. Schematic diagram showing cross-talk between non-genomic ER and Src. ER associates with Src at the cell membrane via a non-genomic mechanism. This leads to an increase in both ERK1/2 and AKT providing a survival advantage. The reduced genomic activity of ER in this setting enhances resistance to tamoxifen. Inhibition of Src with dasatinib causes ER to shuttle to the nucleus where it is targeted by tamoxifen, leading to a decrease in proliferation and re-sensitization to the endocrine agent <b>(B)</b>.</p

Expression of uPA and Caveolin-1 in endocrine-resistant versus sensitive cell lines.

<p>Assessment of mRNA relative expression of uPA and Caveolin-1 using qPCR in wt-MCF7, MCF7-LTED, 1%MCF7 and MCF7-TAMR cell lines. Bars represent ± SEM. *p<0.05, **p<0.01, ***p<0.001.</p

Analysis of src and downstream signalling in response to dasatinib combined with endocrine agents.

<p>Wt-MCF7, MCF7-LTED, 1%MCF7 and MCF7-TAMR cells were treated for 24-hours with the drug combinations mentioned above. Standard concentrations of dasatinib (100nM), E2 (0.01nM), 4-OHT (10nM) and fulvestrant (ICI) (10nM) were used. Whole-cell extracts were assessed for expression of various proteins indicated using immunoblotting. Figures below each panel represent semi-quantitave changes in protein expression relative to actin.</p

Antiproliferative effects of dasatinib alone or in combination with endocrine agents.

<p>Cells were treated with increasing concentrations of dasatinib (<b>A,B</b>), or fixed concentration of dasatinib and increasing concentrations of 4-OHT (<b>C,D</b>) or fulvestrant (ICI) (<b>E,F</b>) for 6-days with a medium change at day 3. Cell viability was determined using CellTitre-Glo^® Luminescent Cell Viability Assay. The data are representative of three independent experiments (Error bars represent ± SEM).</p

Effects of dasatinib on ER-mediated transcription and ER signalling.

<p>Cell lines were co-transfected with EREIItkLuc and pCH110 and treated with the combinations mentioned (<b>A, B, C, D</b>). Standard concentrations of dasatinib (100nM), E2 (0.01nM), 4-OHT (10nM) and fulvestrant (ICI) (10nM), were used. Normalized luciferase activity from triplicate wells was expressed relative to the vehicle-treated control. Bars represent ± SEM. *p<0.05, **p<0.01, ***p<0.001. Effects were confirmed in three independent experiments. Wt-MCF7, MCF7-LTED, 1%MCF7 and MCF7-TAMR cells were treated as indicated for 24-hours, with the same amounts as stated above. Western blot was used to assess changes in total ER and PgR. Figures below each panel represent semi-quantitave changes in protein expression relative to actin.(<b>E</b>).</p

Additional file 3: Table S2. of Cholesterol biosynthesis pathway as a novel mechanism of resistance to estrogen deprivation in estrogen receptor-positive breast cancer

Alteration in transcript levels for genes within the cholesterol biosynthesis pathway

Abundance of larval serum proteins.

<p>Hemolymph was isolated from fed (f) and starved (s) larvae (see Fig. 1). Proteins in samples of 10, 3.3, 1.7 or 1 µl hemolymph were resolved by SDS-PAGE and stained with Coomassie Blue. The position of the major larval serum proteins (LSPs) is indicated by an arrowhead. Position and size (kDa) of molecular weight markers (m) are indicated on the right side.</p

Characterization of the larval hemolymph proteome.

<p>(A) Workflow of the analyses. Hemolymph samples from fed and starved larvae were digested in solution. Tryptic peptides were separated by isoelectric focusing for complexity reduction. Peptides were analyzed using microcapillary liquid chromatography–electrospray ionization–tandem MS (µLC-ESI-MS/MS). SEQUEST spectral search was performed for peptide spectrum matching. (B) Venn diagram illustrating the number of gene models detected in hemolymph from fed and starved larvae, respectively.</p

Summary of identified spectra, peptides, proteins and estimated FDR levels.

a)<p>According to our peptide classification scheme <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0067208#pone.0067208-Qeli1" target="_blank">[38]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0067208#pone.0067208-Grobei1" target="_blank">[46]</a>, class 1a peptides unambiguously identify a single unique protein sequence encoded by a unique transcript. Class 1b peptides also unambiguously identify a unique protein sequence encoded by several transcripts of the same gene model with identical coding region and differences in the 5′ and/or 3′ untranslated regions. Class 2a peptides identify a subset and class 2b peptides all protein sequences encoded by a gene model. Class 3a peptides unambiguously identify one protein sequence, but this sequence could be encoded by several gene models from distinct loci (e.g. histones). Finally, class 3b peptides can be derived from different protein sequences encoded by several gene models from distinct loci and have the least information content.</p>b)<p>For protein groups identified by class 2a or 2b peptides (a gene model identification) all possible protein accessions are listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0067208#pone.0067208.s001" target="_blank">Table S1</a>.</p>c)<p>The minimal number of additional protein identifications by 3b peptides is shown.</p>d)<p>Based on the total hits in target and decoy databases (DB), the FDR was estimated at the spectra, peptide and protein level.</p