Crystallization and evaluation of hen egg-white lysozyme crystals for protein pH titration in the crystalline state

Hen egg-white lysozyme was crystallized over a wide pH range (2.5–8.0) and the quality of the crystals was characterized. Crystallization phase diagrams at pH 2.5, 6.0 and 7.5 were determine

Hydrogen bonds of DsrD protein revealed by neutron crystallography

Hydrogen bonds of DNA-binding protein DsrD have been determined by neutron diffraction. In terms of proton donors and acceptors, DsrD protein shows striking differences from other proteins

Neutron protein crystallography: hydrogen protons and hydration in bio-macromolecules

This text is dedicated to the emerging field of neutron protein crystallography (NPC). It covers all of the practical aspects of NPC and demonstrates how NPC can explore protein features such as hydrogen bonds, protonation and deprotonation of amino acid residues, and hydration structures

Small-Angle Scattering from Irradiated Glassy Carbon

The small-angle neutron scattering from glassy carbon which had been neutron irradiated at 140℃ to a fluence of 1.2×10^ n cm^ has been studied. The void in glassy carbon have a spherical shape nearly uniform in size. Irradiation results in a decrease of density of the matrix but size of the voids are unchanged. Slight shrinkage of the voids is occurred during subsequent annealing above 900℃

Neutron crystallography: opportunities, challenges, and limitations.

International audienceNeutron crystallography has had an important, but relatively small role in structural biology over the years. In this review of recently determined neutron structures, a theme emerges of a field currently expanding beyond its traditional boundaries, to address larger and more complex problems, with smaller samples and shorter data collection times, and employing more sophisticated structure determination and refinement methods. The origin of this transformation can be found in a number of advances including first, the development of neutron image-plates and quasi-Laue methods at nuclear reactor neutron sources and the development of time-of-flight Laue methods and electronic detectors at spallation neutron sources; second, new facilities and methods for sample perdeuteration and crystallization; third, new approaches and computational tools for structure determination