Thermoclinic Assessment Of A Preliminary Circulation Model For Lake George In The Jefferson Project

The Jefferson Project is a collaboration between the Rensselaer Polytechnic Institute, IBM, and the FUND for Lake George aimed at understanding and managing complex factors (road salt, storm water runoff, invasive species) threatening Lake George, New York. Lake George is located about 80 km north of Albany in upstate New York and is known internationally for its water clarity. Understanding the hydrodynamics of the lake is fundamental for creation and maintenance of a research and monitoring program for the early detection of and response to adverse environmental and biological change. In this work a 3D circulation model of the lake is developed to better understand the hydro-environmental conditions of the lake; forcing is by a combination of local public survey data for the water budget and atmospheric data from the NWS (NOAA National Weather Service). The model is validated by a combination of water chemistry data collected by Darrin Fresh Water Institute (DFWI) over the last three decades, and known empirical relationships of the lake\u27s structural profile. Numerical simulations run over several years to capture the seasonal progression of thermocline depth throughout the lake, the south to north salt and surface thermal gradients and the timing of the spring and fall overturn events. Validation is by comparison with physical and chemical measurements collected over the last three decades. The study presents a novel combination of observational data, numerical modelling and empirical relationships to better understand and predict the lake circulation, and consequently the natural ecosystem

Novel Group I Intron in the tRNA(Leu)(UAA) Gene of a γ-Proteobacterium Isolated from a Deep Subsurface Environment

A group I intron has been found to interrupt the anticodon loop of the tRNA(Leu)(UAA) gene in a bacterium belonging to the γ-subdivision of Proteobacteria and isolated from a deep subsurface environment. The subsurface isolate SMCC D0715 was identified as belonging to the genus Pseudomonas. The group I intron from this isolate is the first to be reported for γ-proteobacteria, and the first instance of a tRNA(Leu)(UAA) group I intron to be found in a group of bacteria other than cyanobacteria. The 231-nucleotide (nt) intron's sequence has group I conserved elements and folds into a bona fide group I secondary structure with canonical base-paired segments P1 to P9 and a paired region, P10. The D0715 intron possesses the 11-nt motif CCUACG … UAUGG in its P8 region, a feature not common in bacterial introns. To date, phylogenetic analysis has shown that bacterial introns form two distinct families, and their complex distribution suggests that both lateral transfer and common ancestry have taken part in the evolutionary history of these elements

Development and Utilization of Genetic Probes for Studying Zebra Mussel Veligers

Proceedings of the 1997 Georgia Water Resources Conference, March 20-22, 1997, Athens, Georgia.A key issue in the management of zebra mussel populations is early, rapid, and accurate detection of the planktonic forms of the mussel veligers. In this study, an alternative technology to microscopic enumeration of zebra mussel veligers has been developed based on a species specific genetic probe targeting the 18S rRNA molecule. Probe specificity and sensitivity were determined empirically in the laboratory. The probe did not hybridize to any other bivalve representative tested, including a close relative of the zebra mussel, Mytilopsis leucophaeta. A single veliger could be detected by 18S rRNA targeted probes. The probe was tested in three different field settings including the Hudson River, NY, Lake Champlain, NY/VT, and Lake George, NY and compared to standard methods. Results were generally consistent with microscopy methods. A long term goal of this work is the application of genetic probe technology to the development of a field device for the routine detection and quantification of zebra mussel veligers.Sponsored and Organized by: U.S. Geological Survey, Georgia Department of Natural Resources, The University of Georgia, Georgia State University, Georgia Institute of TechnologyThis book was published by the Institute of Ecology, The University of Georgia, Athens, Georgia 30602 with partial funding provided by the U.S. Department of Interior, Geological Survey, through the Georgia Water Research Institutes Authorization Act of 1990 (P.L. 101-397). The views and statements advanced in this publication are solely those of the authors and do not represent official views or policies of the University of Georgia or the U.S. Geological Survey or the conference sponsors

A molecular perspective on ecological differentiation and biogeography of cyclotrichiid ciliates

Cyclotrichiids are of ecological and evolutionary interest by virtue of their importance in red tide formation, their highly divergent small subunit (SSU) ribosomal RNA (rRNA) genes, kleptoplastidy, and utility as indicators of eutrophication. However, only seven strains have had their SSU rRNA genes sequenced and their environmental diversity and distribution are largely unknown. We probed 67 globally dispersed freshwater column/sediment and soil DNA samples (eDNAs) and constructed 24 environmental gene libraries using polymerase chain reaction primers specific to an uncharacterised cyclotrichiid subgroup. We reveal a novel, globally ubiquitous freshwater clade comprising 25 genetiaclly distinct SSU ribosomal DNA (rDNA) sequences (SSU-types). Some identical SSU-types were detected at globally widely distributed sites. The SSU-types form four distinct phylogenetic clusters according to marine or non-marine provenance, suggesting at least one major marine-freshwater evolutionary transition within the cyclotrichiids. We used the same primers to sample intensively 18 sampling points in 13 closely situated lakes, each characterised by 14 environmental variables, and showed that molecular detection or non-detection of cyclotrichiids was most significantly influenced by levels of total phosphorus, dissolved organic carcon, and chlorophyll a. Within the subset of lakes in which cycloytrichiids were detected, closely related SSU-types differed in their ecological preferences to pH, total phosphorus, and sample depth

Aluminum toxicity risk reduction as a result of reduced acid deposition in Adirondack lakes and ponds

In 1990, the US Congress amended the Clean Air Act (CAA) to reduce regional-scale ecosystem degradation from SOx and NOx emissions which have been responsible for acid deposition in regions such as the Adirondack Mountains of New York State. An ecosystem assessment project was conducted from 1994 to 2012 by the Darrin Fresh Water Institute to determine the effect of these emission reduction policies on aquatic systems. The project investigated water chemistry and biota in 30 Adirondack lakes and ponded waters. Although regulatory changes made in response to the 1990 CAA amendments resulted in a reduction of acid deposition within the Adirondacks, the ecosystem response to these reductions is complicated. A statistical analysis of SO4, pH, Al, and DOC data collected during this project demonstrates positive change in response to decreased deposition. The changes in water chemistry also have lowered the risk of Al toxicity to brook trout (Salvelinus fontinalis [Mitchill]), which allowed the re-introduction of this species to Brooktrout Lake from which it had been extirpated. However, pH and labile aluminum (Alim) fluctuate and are not strongly correlated to changes in acid deposition. As such, toxicity to S. fontinalis also is cyclic and provides rationale for the difficulties inherent in re-establishing resident populations in impacted aquatic environments. Overall, aquatic ecosystems of the Adirondacks show a positive response to reduced deposition driven by changes in environmental policy, but the response is more complex and indicates an ecosystem-wide interaction between aquatic and watershed components of the ecosystem

Bacterial Community Structure of Acid-Impacted Lakes: What Controls Diversity?▿ †

Although it is recognized that acidification of freshwater systems results in decreased overall species richness of plants and animals, little is known about the response of aquatic microbial communities to acidification. In this study we examined bacterioplankton community diversity and structure in 18 lakes located in the Adirondack Park (in the state of New York in the United States) that were affected to various degrees by acidic deposition and assessed correlations with 31 physical and chemical parameters. The pH of these lakes ranged from 4.9 to 7.8. These studies were conducted as a component of the Adirondack Effects Assessment Program supported by the U.S. Environmental Protection Agency. Thirty-one independent 16S rRNA gene libraries consisting of 2,135 clones were constructed from epilimnion and hypolimnion water samples. Bacterioplankton community composition was determined by sequencing and amplified ribosomal DNA restriction analysis of the clone libraries. Nineteen bacterial classes representing 95 subclasses were observed, but clone libraries were dominated by representatives of the Actinobacteria and Betaproteobacteria classes. Although the diversity and richness of bacterioplankton communities were positively correlated with pH, the overall community composition assessed by principal component analysis was not. The strongest correlations were observed between bacterioplankton communities and lake depth, hydraulic retention time, dissolved inorganic carbon, and nonlabile monomeric aluminum concentrations. While there was not an overall correlation between bacterioplankton community structure and pH, several bacterial classes, including the Alphaproteobacteria, were directly correlated with acidity. These results indicate that unlike more identifiable correlations between acidity and species richness for higher trophic levels, controls on bacterioplankton community structure are likely more complex, involving both direct and indirect processes

Metal and proton toxicity to lake zooplankton: a chemical speciation based modelling approach

The WHAM-FTOX model quantifies the combined toxic effects of protons and metal cations towards aquatic organisms through the toxicity function (FTOX), a linear combination of the products of organism-bound cation and a toxic potency coefficient for each cation. We describe the application of the model to predict an observable ecological field variable, species richness of pelagic lake crustacean zooplankton, studied with respect to either acidification or the impacts of metals from smelters. The fitted results give toxic potencies increasing in the order H+ < Al < Cu < Zn < Ni. In general, observed species richness is lower than predicted, but in some instances agreement is close, and is rarely higher than predictions. The model predicts recovery in agreement with observations for three regions, namely Sudbury (Canada), Bohemian Forest (Czech Republic) and a subset of lakes across Norway, but fails to predict observed recovery from acidification in Adirondack lakes (USA)

Brooktrout Lake Case Study: Biotic Recovery from Acid Deposition 20 Years after the 1990 Clean Air Act Amendments

The Adirondack Mountain region is an extensive geographic area (26,305 km²) in upstate New York where acid deposition has negatively affected water resources for decades and caused the extirpation of local fish populations. The water quality decline and loss of an established brook trout (Salvelinus fontinalis [Mitchill]) population in Brooktrout Lake were reconstructed from historical information dating back to the late 1880s. Water quality and biotic recovery were documented in Brooktrout Lake in response to reductions of S deposition during the 1980s, 1990s, and 2000s and provided a unique scientific opportunity to re-introduce fish in 2005 and examine their critical role in the recovery of food webs affected by acid deposition. Using C and N isotope analysis of fish collagen and state hatchery feed as well as Bayesian assignment tests of microsatellite genotypes, we document in situ brook trout reproduction, which is the initial phase in the restoration of a preacidification food web structure in Brooktrout Lake. Combined with sulfur dioxide emissions reductions promulgated by the 1990 Clean Air Act Amendments, our results suggest that other acid-affected Adirondack waters could benefit from careful fish re-introduction protocols to initiate the ecosystem reconstruction of important components of food web dimensionality and functionality