Role of director of a kindergarten in shaping school culture

Thesis deals with the role of the director of a kindergarten within shaping of the school culture and with the potential of school culture in terms of contemporary education. Thesis focuses on the factors which the director influences the most and which leads to increased level of quality of teachers' work. In the theoretical part the emphasis is on understanding the concepts in the school culture, school climate, management and leadership and also on relationships in kindergarten, which create and develop a quality society. Further, the thesis concerns the research investigation, which detects the orientation of the issue of school culture and current use of knowledge in connection to the management of kindergarten. Key words Kindergarten surrounding, communication, kindergarten culture, interpersonal relationships, labor relations, director, employee satisfaction, styles of management

Table_1_Unbiased screen for pathogens in human paraffin-embedded tissue samples by whole genome sequencing and metagenomics.xlsx

Identification of bacterial pathogens in formalin fixed, paraffin embedded (FFPE) tissue samples is limited to targeted and resource-intensive methods such as sequential PCR analyses. To enable unbiased screening for pathogens in FFPE tissue samples, we established a whole genome sequencing (WGS) method that combines shotgun sequencing and metagenomics for taxonomic identification of bacterial pathogens after subtraction of human genomic reads. To validate the assay, we analyzed more than 100 samples of known composition as well as FFPE lung autopsy tissues with and without histological signs of infections. Metagenomics analysis confirmed the pathogenic species that were previously identified by species-specific PCR in 62% of samples, showing that metagenomics is less sensitive than species-specific PCR. On the other hand, metagenomics analysis identified pathogens in samples, which had been tested negative for multiple common microorganisms and showed histological signs of infection. This highlights the ability of this assay to screen for unknown pathogens and detect multi-microbial infections which is not possible by histomorphology and species-specific PCR alone.</p

DataSheet_1_Unbiased screen for pathogens in human paraffin-embedded tissue samples by whole genome sequencing and metagenomics.pdf

Identification of bacterial pathogens in formalin fixed, paraffin embedded (FFPE) tissue samples is limited to targeted and resource-intensive methods such as sequential PCR analyses. To enable unbiased screening for pathogens in FFPE tissue samples, we established a whole genome sequencing (WGS) method that combines shotgun sequencing and metagenomics for taxonomic identification of bacterial pathogens after subtraction of human genomic reads. To validate the assay, we analyzed more than 100 samples of known composition as well as FFPE lung autopsy tissues with and without histological signs of infections. Metagenomics analysis confirmed the pathogenic species that were previously identified by species-specific PCR in 62% of samples, showing that metagenomics is less sensitive than species-specific PCR. On the other hand, metagenomics analysis identified pathogens in samples, which had been tested negative for multiple common microorganisms and showed histological signs of infection. This highlights the ability of this assay to screen for unknown pathogens and detect multi-microbial infections which is not possible by histomorphology and species-specific PCR alone.</p