Differential expression of microRNAs following cardiopulmonary bypass in children with congenital heart diseases

Background: Children with congenital heart defects (CHDs) are at high risk for myocardial failure after operative procedures with cardiopulmonary bypass (CPB). Recent studies suggest that microRNAs (miRNA) are involved in the development of CHDs and myocardial failure. Therefore, the aim of this study was to determine alterations in the miRNA profle in heart tissue after cardiac surgery using CPB. Methods: In total, 14 tissue samples from right atrium were collected from patients before and after connection of the CPB. SurePrint™ 8 × 60K Human v21 miRNA array and quantitative reverse transcription-polymerase chain reac‑ tion (RT-qPCR) were employed to determine the miRNA expression profle from three patients before and after con‑ nection of the CPB. Enrichment analyses of altered miRNA expression were predicted using bioinformatic tools. Results: According to miRNA array, a total of 90 miRNAs were signifcantly altered including 29 miRNAs with increased and 61 miRNAs with decreased expression after de-connection of CPB (n = 3) compared to before CPB (n = 3). Seven miRNAs had been validated using RT-qPCR in an independent cohort of 11 patients. Enrichment analyses applying the KEGG database displayed the highest correlation for signaling pathways, cellular community, cardiovascular disease and circulatory system. Conclusion: Our result identifed the overall changes of the miRNome in right atrium tissue of patients with CHDs after CPB. The diferentially altered miRNAs lay a good foundation for further understanding of the molecular function of changed miRNAs in regulating CHDs and after CPB in particula

Sentiment-based commercial real estate forecasting with Google search volume data

Purpose – The purpose of this paper is to examine internet search query data provided by “Google Trends”, with respect to its ability to serve as a sentiment indicator and improve commercial real estate forecasting models for transactions and price indices. Design/methodology/approach – This paper examines internet search query data provided by “Google Trends”, with respect to its ability to serve as a sentiment indicator and improve commercial real estate forecasting models for transactions and price indices. Findings – The empirical results show that all models augmented with Google data, combining both macro and search data, significantly outperform baseline models which abandon internet search data. Models based on Google data alone, outperform the baseline models in all cases. The models achieve a reduction over the baseline models of the mean squared forecasting error for transactions and prices of up to 35 and 54 per cent, respectively. Practical implications – The results suggest that Google data can serve as an early market indicator. The findings of this study suggest that the inclusion of Google search data in forecasting models can improve forecast accuracy significantly. This implies that commercial real estate forecasters should consider incorporating this free and timely data set into their market forecasts or when performing plausibility checks for future investment decisions. Originality/value – This is the first paper applying Google search query data to the commercial real estate sector

The Dictyostelium Bcr/Abr-related protein DRG regulates both Rac- and Rab-dependent pathways

Dictyostelium discoideum DdRacGap1 (DRG) contains both Rho-GEF and Rho-GAP domains, a feature it shares with mammalian Bcr and Abr. To elucidate the physiological role of this multifunctional protein, we characterized the enzymatic activity of recombinant DRG fragments in vitro, created DRG-null cells, and studied the function of the protein in vivo by analysing the phenotypic changes displayed by DRG-depleted cells and DRG-null cells complemented with DRG or DRG fragments. Our results show that DRG-GEF modulates F-actin dynamics and cAMP-induced F-actin formation via Rac1-dependent signalling pathways. DRG’s RacE-GAP activity is required for proper cytokinesis to occur. Additionally, we provide evidence that the specificity of DRG is not limited to members of the Rho family of small GTPases. A recombinant DRG-GAP accelerates the GTP hydrolysis of RabD 30-fold in vitro and our complementation studies show that DRG-GAP activity is required for the RabD-dependent regulation of the contractile vacuole system in Dictyostelium

A Bcr/Abr-related protein controls the organization of a tubolocisternal organelle, F-Actin dynamics, and cytokinesis in Dictyostelium

Dictyostelium discoideum DdRacGap1 (DRG) contains both Rho-GEF and Rho-GAP domains, a feature it shares with mammalian Bcr and Abr. To elucidate the physiological role of this multifunctional protein, we characterized the enzymatic activity of recombinant DRG fragments in vitro, created DRG-null cells, and studied the function of the protein in vivo by analysing the phenotypic changes displayed by DRG-depleted cells and DRG-null cells complemented with DRG or DRG fragments. Our results show that DRG-GEF modulates F-actin dynamics and cAMP-induced F-actin formation via Rac1-dependent signalling pathways. DRGs RacE-GAP activity is required for proper cytokinesis to occur. Additionally, we provide evidence that the specificity of DRG is not limited to members of the Rho family of small GTPases. A recombinant DRG-GAP accelerates the GTP hydrolysis of RabD 30-fold in vitro and our complementation studies show that DRG-GAP activity is required for the RabD-dependent regulation of the contractile vacuole system in Dictyostelium