Cultivar diversity as a means of ecologically intensifying dry matter production in a perennial forage stand

The relationship between genotypic diversity and productivity has not been adequately explored in perennial forage production systems despite strong theoretical and empirical evidence supporting diversity\u27s role in ecosystem functioning in other managed and unmanaged systems. We conducted a two-year field experiment with six cultivars of an agriculturally important forage grass, Lolium perenne L. (perennial ryegrass). Dry matter production of L. perenne and the weed community that emerged from the soil seed bank were measured each year in treatments that ranged from cultivar monocultures to three- and six-way cultivar mixtures, all sown at a constant seeding rate. Mean L. perenne dry matter production increased with increasing cultivar diversity and was highest in mixtures that contained cultivars representing the greatest additive trait range (calculated on rankings of three traits: winter hardiness, heading date, and tolerance to grazing). Mixtures had greater yields than those predicted by the mean of their component monoculture yields, but there was evidence that highly productive cultivars may have dampened over-yielding in mixtures. Weed abundance was correlated with L. perenne dry matter, but not L. perenne cultivar diversity. These results suggest that multi-cultivar mixtures may have utility as an approach to ecologically intensifying perennial forage production. Additional research will be necessary to determine the mechanisms responsible for the over-yielding observed in this study and the generality of these findings

Transition region and chromospheric signatures of impulsive heating events. II. Modeling

Results from the Solar Maximum Mission showed a close connection between the hard X-ray (HXR) and transition region (TR) emission in solar flares. Analogously, the modern combination of RHESSI and IRIS data can inform the details of heating processes in ways that were never before possible. We study a small event that was observed with RHESSI, IRIS, SDO, and Hinode, allowing us to strongly constrain the heating and hydrodynamical properties of the flare, with detailed observations presented in a previous paper. Long duration redshifts of TR lines observed in this event, as well as many other events, are fundamentally incompatible with chromospheric condensation on a single loop. We combine RHESSI and IRIS data to measure the energy partition among the many magnetic strands that comprise the flare. Using that observationally determined energy partition, we show that a proper multithreaded model can reproduce these redshifts in magnitude, duration, and line intensity, while simultaneously being well constrained by the observed density, temperature, and emission measure. We comment on the implications for both RHESSI and IRIS observations of flares in general, namely that: (1) a single loop model is inconsistent with long duration redshifts, among other observables; (2) the average time between energization of strands is less than 10 s, which implies that for a HXR burst lasting 10 minutes, there were at least 60 strands within a single IRIS pixel located on the flare ribbon; (3) the majority of these strands were explosively heated with an energy distribution well described by a power law of slope »-1.6; (4) the multi-stranded model reproduces the observed line profiles, peak temperatures, differential emission measure distributions, and densities

Time-Varying Input and State Delay Compensation for Uncertain Nonlinear Systems

A robust controller is developed for uncertain, second-order nonlinear systems subject to simultaneous unknown, time-varying state delays and known, time-varying input delays in addition to additive, sufficiently smooth disturbances. An integral term composed of previous control values facilitates a delay-free open-loop error system and the development of the feedback control structure. A stability analysis based on Lyapunov-Krasovskii (LK) functionals guarantees uniformly ultimately bounded tracking under the assumption that the delays are bounded and slowly varying

Increased Productivity of a Cover Crop Mixture Is Not Associated with Enhanced Agroecosystem Services

Cover crops provide a variety of important agroecological services within cropping systems. Typically these crops are grown as monocultures or simple graminoid-legume bicultures; however, ecological theory and empirical evidence suggest that agroecosystem services could be enhanced by growing cover crops in species-rich mixtures. We examined cover crop productivity, weed suppression, stability, and carryover effects to a subsequent cash crop in an experiment involving a five-species annual cover crop mixture and the component species grown as monocultures in SE New Hampshire, USA in 2011 and 2012. The mean land equivalent ratio (LER) for the mixture exceeded 1.0 in both years, indicating that the mixture over-yielded relative to the monocultures. Despite the apparent over-yielding in the mixture, we observed no enhancement in weed suppression, biomass stability, or productivity of a subsequent oat (Avena sativa L.) cash crop when compared to the best monoculture component crop. These data are some of the first to include application of the LER to an analysis of a cover crop mixture and contribute to the growing literature on the agroecological effects of cover crop diversity in cropping systems

The Magnetic Properties of Heating Events on High-Temperature Active Region Loops

Understanding the relationship between the magnetic field and coronal heating is one of the central problems of solar physics. However, studies of the magnetic properties of impulsively heated loops have been rare. We present results from a study of 34 evolving coronal loops observed in the Fe XVIII line component of AIA/SDO 94 A filter images from three active regions with different magnetic conditions. We show that the peak intensity per unit cross-section of the loops depends on their individual magnetic and geometric properties. The intensity scales proportionally to the average field strength along the loop ($B_{avg}$) and inversely with the loop length ($L$) for a combined dependence of $(B_{avg}/L)^{0.52\pm0.13}$. These loop properties are inferred from magnetic extrapolations of the photospheric HMI/SDO line-of-sight and vector magnetic field in three approximations: potential and two Non Linear Force-Free (NLFF) methods. Through hydrodynamic modeling (EBTEL model) we show that this behavior is compatible with impulsively heated loops with a volumetric heating rate that scales as $\epsilon_H\sim B_{avg}^{0.3\pm0.2}/L^{0.2\pm^{0.2}_{0.1}}$.Comment: Astrophysical Journal, in pres

Cellular delivery of antibodies: effective targeted subcellular imaging and new therapeutic tool

It is already more than a century since the pioneering work of the Nobel Laureate Ehrlich gave birth to the side chain theory1, which helped to define antibodies and their ability to target specific biological sites. However, the use of antibodies is still restricted to the extracellular space due to the lack of a suitable delivery vehicle for the efficient transport of antibodies into live cells without inducing toxicity. In this work, we report the efficient encapsulation and delivery of antibodies into live cells with no significant loss of cell viability or any deleterious affect on the cell metabolic activity. This delivery system is based on poly(2-(methacryloyloxy)ethyl phosphorylcholine)-block-(2-(diisopropylamino)ethyl methacrylate), (PMPC-PDPA), a pH sensitive diblock copolymer that self-assembles to form nanometer-sized vesicles, also known as polymersomes, at physiological pH. These polymersomes can successfully deliver relatively high antibody payloads within live cells. Once inside the cells, we demonstrate that these antibodies can target their epitope by immune-labelling of cytoskeleton, Golgi, and transcription factor proteins in live cells. We also demonstrate that this effective antibody delivery mechanism can be used to control specific subcellular events, as well as modulate cell activity and pro-inflammatory process