Application of the TDMA Technique Toward the Size and Charge Distribution Measurement of Graphite, Gold, Palladium, and Silver Aerosols

The knowledge of charge distributions among aerosol particles has been an important topic for many years because of the strong electrostatic interactions which can greatly influence aerosol transport and evolution. Theoretical models have been developed although experimental verification has been limited because of the difficulty in measuring charged aerosols. Recently a method utilizing a tandem differential mobility analyzer (TDMA) has been shown to be applicable toward measuring both the size and charge distributions of nanosized combustion aerosols. The goal of this work is on further exploration of this method toward the measurement of non-combustion aerosols and in particular those associated with very high temperature reactors (VHTRs). The complete bipolar charge and size distributions of spark generated graphite, gold, silver, and palladium aerosol have been measured with a TDMA apparatus assembled and calibrated during this study. In addition, an electrostatic precipitator has been designed and constructed for measuring the size distributions of neutrally charged particles associated with these aerosols. The results show charge asymmetry in all measured aerosols with higher concentrations of positively charged particles than negative at the same charge level. These results differ from equilibrium charge distributions of both Boltzmann and Fuchs showing that charge equilibrium may not always be an appropriate assumption. The TDMA technique should find applications in characterizing VHTR aerosols and rate processes such as coagulation, deposition, and resuspension which will be important for both reactor design, and accident modeling and simulation

Structure and Functional Analysis of the RNA- and Viral Phosphoprotein-Binding Domain of Respiratory Syncytial Virus M2-1 Protein

Respiratory syncytial virus (RSV) protein M2-1 functions as an essential transcriptional cofactor of the viral RNA-dependent RNA polymerase (RdRp) complex by increasing polymerase processivity. M2-1 is a modular RNA binding protein that also interacts with the viral phosphoprotein P, another component of the RdRp complex. These binding properties are related to the core region of M2-1 encompassing residues S58 to K177. Here we report the NMR structure of the RSV M2-158–177 core domain, which is structurally homologous to the C-terminal domain of Ebola virus VP30, a transcription co-factor sharing functional similarity with M2-1. The partial overlap of RNA and P interaction surfaces on M2-158–177, as determined by NMR, rationalizes the previously observed competitive behavior of RNA versus P. Using site-directed mutagenesis, we identified eight residues located on these surfaces that are critical for an efficient transcription activity of the RdRp complex. Single mutations of these residues disrupted specifically either P or RNA binding to M2-1 in vitro. M2-1 recruitment to cytoplasmic inclusion bodies, which are regarded as sites of viral RNA synthesis, was impaired by mutations affecting only binding to P, but not to RNA, suggesting that M2-1 is associated to the holonucleocapsid by interacting with P. These results reveal that RNA and P binding to M2-1 can be uncoupled and that both are critical for the transcriptional antitermination function of M2-1

Compte rendu relatif au Post-Doctorat à San Diego depuis Septembre 2008

Nicaise Virginie. Compte rendu relatif au Post-Doctorat à San Diego depuis Septembre 2008. In: Diplômées, n°230, 2009. pp. 181-182

Compte-rendu relatif au Post-Doctorat à San Diego depuis Septembre 2008

Nicaise Virginie. Compte-rendu relatif au Post-Doctorat à San Diego depuis Septembre 2008. In: Diplômées, n°229, 2009. Les femmes et l'argent. p. 104

La compétition : Moteur ou obstacle à la pratique de l'activité ?

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La compétition : Moteur ou obstacle à la pratique de l'activité ?

International audienc

Parental profiles and their effects on elite adolescent athletes' self-determination theory variables

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Les feedback émis par l'enseignant(e) d'EPS et perçus par les élèves : quelle similarité ?

Analyse des différences sexuées relatives aux interactions observées en classe entre l'enseignant et l'élève lors d'une séance d'EPS (activité badminton et basket-ball) et de leurs perceptions en fonction du sexe de l'élèv

Les feedback de l enseignant-e en EPS (perceptions des élèves et observation)

Cette thèse s intéresse aux perceptions par les élèves des feedback de l enseignant-e en cours d Education Physique et Sportive (EPS). Il s agit de mettre en évidence si ces perceptions sont différentes entre les filles et les garçons quel que soit leur niveau en EPS et leur compétence physique perçue. Plusieurs variables modératrices sont explorées telles que le sexe de l enseignant, l activité physique enseignée, la proportion des filles et garçons dans la classe et le niveau en EPS de l élève. Par ailleurs il est aussi de confronter ces perceptions aux observations réelles en classe (37 séances filmées). Les modèles de médiation cognitive insistent sur le rôle important des cognitions et perceptions par les élèves des comportements de l enseignant-e pour mieux comprendre les effets des feedback de l enseignant-e sur les perceptions de soi et la performance de l élève. Dans une série de 5 études engageant près de 1000 lycéen-nes, nous envisageons relever les perceptions par les filles et les garçons des feedback de l enseignant-e d EPS, les comportements réels de l enseignant-e et tenter d évaluer la cohérence entre les mesures perçues et observées des feedbackThis doctoral dissertation focused on students perceptions of physical education (PE) teacher s feedback. The cognitive mediational models of instruction suggest that student cognitions must be examined to better understand the effects of teacher s feedback on the psychological responses and performance. The aim was to explore whether these perceptions are gender differentiated whatever student s PE level. Several moderator variables have been investigated such as teacher s gender, physical activity, gender composition of the class and student s PE level. Otherwise, observations of PE teacher were realized with 37 lessons. Five studies involving near to 1000 high school students were carried out. We measured girls and boys perceptions of PE teacher s behaviours, teacher s behaviours toward them and the congruency between the two measuresLYON1-BU.Sciences (692662101) / SudocSudocFranceF

Promotion de l’activité physique auprès d’adolescents sédentaires

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