Streptococcus suis, an Important Cause of Adult Bacterial Meningitis in Northern Vietnam

Streptococcus suis can cause severe systemic infection in adults exposed to infected pigs or after consumption of undercooked pig products. S. suis is often misdiagnosed, due to lack of awareness and improper testing. Here we report the first fifty cases diagnosed with S. suis infection in northern Viet Nam.In 2007, diagnostics for S. suis were set up at a national hospital in Hanoi. That year there were 43 S. suis positive cerebrospinal fluid samples, of which S. suis could be cultured in 32 cases and 11 cases were only positive by PCR. Seven patients were blood culture positive for S. suis but CSF culture and PCR negative; making a total of 50 patients with laboratory confirmed S. suis infection in 2007. The number of S. suis cases peaked during the warmer months.S. suis was commonly diagnosed as a cause of bacterial meningitis in adults in northern Viet Nam. In countries where there is intense and widespread exposure of humans to pigs, S. suis can be an important human pathogen

Severe Pandemic H1N1 2009 Infection Is Associated with Transient NK and T Deficiency and Aberrant CD8 Responses

BACKGROUND: It is unclear why the severity of influenza varies in healthy adults or why the burden of severe influenza shifts to young adults when pandemic strains emerge. One possibility is that cross-protective T cell responses wane in this age group in the absence of recent infection. We therefore compared the acute cellular immune response in previously healthy adults with severe versus mild pandemic H1N1 infection. METHODS AND PRINCIPAL FINDINGS: 49 previously healthy adults admitted to the National Hospital of Tropical Diseases, Viet Nam with RT-PCR-confirmed 2009 H1N1 infection were prospectively enrolled. 39 recovered quickly whereas 10 developed severe symptoms requiring supplemental oxygen and prolonged hospitalization. Peripheral blood lymphocyte subset counts and activation (HLADR, CD38) and differentiation (CD27, CD28) marker expression were determined on days 0, 2, 5, 10, 14 and 28 by flow cytometry. NK, CD4 and CD8 lymphopenia developed in 100%, 90% and 60% of severe cases versus 13% (p<0.001), 28%, (p = 0.001) and 18% (p = 0.014) of mild cases. CD4 and NK counts normalized following recovery. B cell counts were not significantly associated with severity. CD8 activation peaked 6-8 days after mild influenza onset, when 13% (6-22%) were HLADR+CD38+, and was accompanied by a significant loss of resting/CD27+CD28+ cells without accumulation of CD27+CD28- or CD27-CD28- cells. In severe influenza CD8 activation peaked more than 9 days post-onset, and/or was excessive (30-90% HLADR+CD38+) in association with accumulation of CD27+CD28- cells and maintenance of CD8 counts. CONCLUSION: Severe influenza is associated with transient T and NK cell deficiency. CD8 phenotype changes during mild influenza are consistent with a rapidly resolving memory response whereas in severe influenza activation is either delayed or excessive, and partially differentiated cells accumulate within blood indicating that recruitment of effector cells to the lung could be impaired

Diagnosis of 50 S. suis cases in Hanoi in 2007.

*<p>Positive cerebrospinal fluid cases were also regularly positive by blood culture (data not shown).</p

PFGE after SmaI digestion of Streptococcus suis serotype 2 strains isolated from patients in northern Vietnam.

<p>A dendrogram was generated by Dice analysis (optimization, 0.5%; band tolerance, 1.5%) and cluster analysis with unweighted pair group method with arithmetic mean, using Bionumerics software (Applied Maths). Bars indicate 95% CIs. Four representative strains from the main clusters in southern Viet Nam (A, B, C, and D) were included. Also reference strains were included (89–151 and 31533). Strains with the annotation old are from before 2007.</p

Location and seasonality of S. suis infections.

<p>(A) Location of residence of 50 human S. suis cases diagnosed in northern Viet Nam in 2007 (black dot is one case) and pig density; (B) Month of diagnosis of S. suis cases during 2007.</p

Detection of influenza virus RNA in respiratory specimens from patients with severe versus mild influenza.

<p>Nose and throat swabs were assessed for the presence of viral RNA as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031535#s4" target="_blank">methods</a>. Red dots/lines indicate the first and last days from fever onset when viral RNA was detected, the first day being when the first sample was collected. Blue dots represent the last day that samples were tested.</p

Activation and differentiation marker expression by CD8 T cells from patients with severe versus mild influenza.

<p>Panels a–f show the percentages of cells expressing the markers indicated on the vertical axis by time interval since onset. Each line represents an individual patient. Panel g shows the relationship between the percentages of CD8 T cells that are CD38+HLADR+ and CD27+CD28− and includes results for all time points. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031535#s2" target="_blank">Results</a> are shown for 10 patients with severe influenza (red tones symbols and lines) and 39 patients with mild influenza (blue tone symbols and lines).</p

Activation, differentiation and extravasation marker expression by CD8 T cells over the course of mild influenza A illness.

<p>The percentages (a) and absolute count (b) of CD8 T cells with the phenotypes CXCR3^high (green lines); CD27+CD28+ (brown lines); CD27+CD28−(red lines); CD27−CD28− (yellow lines); CD38+HLA-DR- (light blue lines) and CD38+HLADR+ (dark blue lines) is shown for 39 patients with mild influenza illness at different time intervals post-fever onset. The number tested during each time interval was 28, 34, 39, 19 and 9. Asterisks indicate where values differ significantly via paired t-Test from levels at recovery (*), or at recovery and day 1–3 (**), or at recovery and at earlier time intervals (***).</p

Patient characteristics.

<p>*adapted PMEWS = adapted pandemic medical early warning score as defined in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031535#s4" target="_blank">methods</a>.</p