Cosmological Constant and Gravitational Repulsion Effect: 1. Homogeneous models with radiation

Within the framework of the minimum quadratic Poincare gauge theory of gravity in the Riemann-Cartan spacetime we study the influence of gravitational vacuum energy density (a cosmological constant) on the dynamics of various gravitating systems. It is shown that the inclusion of the cosmological term can lead to gravitational repulsion. For some simple cases of spatially homogeneous cosmological models with radiation we obtain non-singular solutions in form of elementary functions and elliptic integrals.Comment: REVTEX, 11 pages, Syracuse University preprint SU-GP-93/5-

Individual Resilience, Social Support, and Health Risk Behaviors in Adolescents and Young Adults: Study on Cross-sectional and Longitudinal Samples

Resilience is considered the ability that a person gains positive achievements despite exposure to significantly adverse life conditions. However, a majority of previous research has focused on human developmental tasks or academic achievements. Evidence of resilience on health risk behaviors has not been comprehensively established yet. The purposes of this dissertation were to extend the existing literature about the stability of resilience through time, to examine how the resilience scale concurrently and prospectively predict resilience statuses, and to explore effects of Social support over a long period of lifetime. The dissertation used a secondary database from the public-use version of the National Longitudinal Study of Adolescent Health (Add Health). Resilience status was defined as a lack of health risk behaviors of suicide, violence and substance use. Resilience scale was constructed by using 35 items from the Add Health data. Data were analyzed data were analyzed using logistic regression and Generalized Estimating Equations (GEE) models. The results showed that resilience status in general and adversity populations varied over time. However, when viewed over a long period of lifetime, individual resiliency on health risk behaviors appears to be rather stable. The resilience scale and its subscales strongly predicted resilience status at a given point in time or over time. Parental support had little or no effect on resilience status, having more number of close friends increased the risk of some health behaviors, and religious services led participants to become more resilient on some health risk behaviors. This research will provide policy makers better knowledge about the role of resilience on health risk behaviors during the transition from adolescence to adulthood. The findings may contribute to the existing literature by extending the knowledge of how resilience on the domain of health risk behaviors changes over time and which factors influence on the resilience process

Status of acute hepatopancreatic necrosis disease (AHPND) and other emerging diseases of penaeid shrimps in Viet Nam

Acute hepatopancreatic necrosis disease (AHPND), formerly called early mortality syndrome (EMS), was first reported in 2010 among penaeid shrimps cultivated in the Mekong Delta Region of Viet Nam albeit without any laboratory confirmation. The disease subsequently spread to a wide range of shrimp production areas in the same region (Soc Trang: 1,719 ha; Bac Lieu: 346 ha; and Ca Mau: 3,493 ha), so that the Government of Viet Nam requested for technical assistance from the Food and Agriculture Organization (FAO) of the United Nations in 2011. In 2012, FAO supported Viet Nam through the project TCP/VIE/3304 Emergency assistance to control the spread of an unknown disease affecting shrimps in Viet Nam, under which the Department of Animal Health of Viet Nam (DAH) collaborated with the University of Arizona and FAO experts to carry out indepth studies to identify the etiologic agent of the disease. As a result, unique isolates of Vibrio parahaemolyticus was identified as the causative agent of AHPND in 2013. Viet Nam has been vigilant and transparent with regard to aquatic animal diseases through official notifications to the World Organization for Animal Health (OIE) and the Network of Aquaculture Centres in Asia-Pacific (NACA). AHPND outbreaks have no clear temporal pattern with black tiger (Penaeus monodon) and whiteleg (P. vannamei) shrimps showing similar incidence risk. The disease occurs at any stage of shrimp cultivation, i.e. on average about 35 days after stocking. To date, unwarranted outbreaks of AHPND in major shrimp-producing provinces in Viet Nam have been apparently regulated. Aside from AHPND, white spot disease (WSD) has also been a persistent problem responsible for serious economic losses in many shrimp-producing areas in Viet Nam. To prevent and control the further spread of infectious diseases of shrimps including AHPND and WSD, multiple control measures have been implemented including guidance of farmers to improve production conditions, facilities and biosecurity application, active surveillance of shrimp production areas for early warning, screening of broodstock and postlarvae for any OIE listed diseases, regulation on movement of stocks, and collaboration with regional and international organizations in carrying out in-depth epidemiological studies that will be needed in the formulation of pragmatic and holistic disease interventions

Determinants Influencing Vietnamese Farmers’ Intention in Applying New Technologies in Agricultural Farming

This paper used survey data from 273 farmers planting fruit trees in 23 provinces to assess the impact of some factors on the intention of applying new technologies to agricultural production of Vietnamese farmers. Research results have identified and measured a number of factors that have a positive impact and some negative factors hinder farmers' intention to apply new technologies. These findings are the basis to recommend policies and solutions to promote Vietnamese farmers to apply new technologies. Keywords: Agricultural farming; Applicability, Belief; Benefit; Market demand; Communication DOI: 10.7176/EJBM/12-6-06 Publication date: February 29th 2020

Decoding the capability of Lactobacillus plantarum W1 isolated from soybean whey in producing an exopolysaccharide

This study aims at producing exopolysaccharides (EPS) from a lactic acid bacterial strain. The soybean whey-isolated Lactobacillus plantarum W1 (EPS-W1), which belongs to genus Lactobacillus, is identified using the phenylalanyl-tRNA sequencing method. Of all the examined strains, R-49778 (as numbered by BCCM/LMG Bacteria Collection, Ghent University, Belgium) showed the highest capability of producing exopoly-saccharides. Structural characterization revealed a novel exopolysaccharide consisting of repeating units of -> 6)-D-Glcp-(1 ->; -> 3)-D-Manp-(1 ->; -> 3)-6-Glcp-(1 -> and a branch of -> 6)-D-Manp-(1 ->; -> 2)-D-Glcp-(1 ->. This discovery opens up avenues for the production of EPS for food industries, functional foods, and biomedical applications

In silico analysis of hypermethylation of E-cadherin gene promoter in Nasopharyngeal carcinoma

Background: DNA hypermethylation changes in CpG islands of promoter region of E-cadherin (E-cad) gene, one of the tumor suppressor genes, have been described to be involved the formation and progression of human nasopharyngeal carcinoma, which is the most common and highly incident cancer of head and neck cancer in Asian countries, especially in Vietnam. Purpose: In Vietnam, there is still no research about E-cad promoter methylation in NPC, thus, in current report, a systematic literature revision was carried out to summary the current evidences about the frequencies of Ecad gene promoter on NPC for further applied in Vietnamese population. Methods: A systematic literature analysis was conducted based on the comprehensive search of observational studies. Moreover, CpG islands of candidate gene and transcriptional factors were predicted by using many bioinformatics tools, such as Methprimer, TFsearch, etc. Results: Total of 9 previous published studies were identified and accessed for eligibility from the literature research and enrolled into systematic revision. The variants of E-cad hypermethylation frequency ranked from 11.0% to 64.55% were observed. Moreover, the average weight frequencies of methylated and unmethylated E-cad gene promoter were 55.46% and 40.78%, respectively. Moreover, by several bioinformatics tools, we were successful in predicting the CpG islands as well as identifing transcriptional factor binding sites, served as “hot spot” for ideal primer pick up, located in candidate gene promoter. Conclusion: Based on these data, it suggested that the hypermethylation of E-cad gene promoter was a significant characteristic of NPC, in which, it could be further applied in evaluation of E-cad gene promoter status in Vietnamese population

Novel exopolysaccharide produced from fermented bamboo shoot-isolated Lactobacillus fermentum

This study aimed at providing a route towards the production of a novel exopolysaccharide (EPS) from fermented bamboo shoot-isolated Lactobacillus fermentum. A lactic acid bacteria strain, with high EPS production ability, was isolated from fermented bamboo shoots. This strain, R-49757, was identified in the BCCM/LMG Bacteria Collection, Ghent University, Belgium by the phenylalanyl-tRNA synthetase gene sequencing method, and it was named Lb. fermentum MC3. The molecular mass of the EPS measured via gel permeation chromatography was found to be 9.85 × 104 Da. Moreover, the monosaccharide composition in the EPS was analyzed by gas chromatography–mass spectrometry. Consequently, the EPS was discovered to be a heteropolysaccharide with the appearance of two main sugars—D-glucose and D-mannose—in the backbone. The results of one-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance spectroscopy analyses prove the repeating unit of this polysaccharide to be [→6)-β-D-Glcp-(1→3)-β-D-Manp-(1→6)-β-D-Glcp-(1→]n, which appears to be a new EPS. The obtained results open up an avenue for the production of novel EPSs for biomedical applications

Portable Rabies Virus Sequencing in Canine Rabies Endemic Countries Using the Oxford Nanopore MinION

As countries with endemic canine rabies progress towards elimination by 2030, it will become necessary to employ techniques to help plan, monitor, and confirm canine rabies elimination. Sequencing can provide critical information to inform control and vaccination strategies by identifying genetically distinct virus variants that may have different host reservoir species or geographic distributions. However, many rabies testing laboratories lack the resources or expertise for sequencing, especially in remote or rural areas where human rabies deaths are highest. We developed a low-cost, high throughput rabies virus sequencing method using the Oxford Nanopore MinION portable sequencer. A total of 259 sequences were generated from diverse rabies virus isolates in public health laboratories lacking rabies virus sequencing capacity in Guatemala, India, Kenya, and Vietnam. Phylogenetic analysis provided valuable insight into rabies virus diversity and distribution in these countries and identified a new rabies virus lineage in Kenya, the first published canine rabies virus sequence from Guatemala, evidence of rabies spread across an international border in Vietnam, and importation of a rabid dog into a state working to become rabies-free in India. Taken together, our evaluation highlights the MinION’s potential for low-cost, high volume sequencing of pathogens in locations with limited resources

Transgenic Mice for Intersectional Targeting of Neural Sensors and Effectors with High Specificity and Performance

SummaryAn increasingly powerful approach for studying brain circuits relies on targeting genetically encoded sensors and effectors to specific cell types. However, current approaches for this are still limited in functionality and specificity. Here we utilize several intersectional strategies to generate multiple transgenic mouse lines expressing high levels of novel genetic tools with high specificity. We developed driver and double reporter mouse lines and viral vectors using the Cre/Flp and Cre/Dre double recombinase systems and established a new, retargetable genomic locus, TIGRE, which allowed the generation of a large set of Cre/tTA-dependent reporter lines expressing fluorescent proteins, genetically encoded calcium, voltage, or glutamate indicators, and optogenetic effectors, all at substantially higher levels than before. High functionality was shown in example mouse lines for GCaMP6, YCX2.60, VSFP Butterfly 1.2, and Jaws. These novel transgenic lines greatly expand the ability to monitor and manipulate neuronal activities with increased specificity.Video Abstrac