Developing Rural Tourism with the Participation of Related Parties in Bac Kan Province

Tourism development policy will pay attention to stakeholders, especially the people, who play an important role. However, in reality, when people participate in rural tourism development, they are not equipped with basic knowledge and skills in managing and serving tourists. Because the benefits between stakeholders are still not clear, the participation of people in rural tourism development is currently only passive. Active participation need to be directed from the Government and local authorities because the concept that rural tourism development is not only about creating economic benefits but also associated with conservation and preservation, together with keeping and promoting cultural - historical values in rural areas

Isolation and characterization of chlorpyrifos-degrading bacteria in tea-growing soils

The excess use of pesticides in the agricultural sector has caused environmental pollution and affected the complete ecosystem. Among the various commonly used pesticides, chlorpyrifos (CPF) is widely used against multiple agrarian pests due to its effectiveness and higher insecticidal activities. However, along with its beneficial usage, CPF has various residual effects on the environment, causing multiple negative impacts on aquatic organisms and human health. Consequently, methods for eliminating CPF in the background are essential. Among the currently available approaches to CPF remediation, biological methods using microorganisms are eco-friendly and cost-effective. Therefore, this study was conducted to isolate and characterize chlorpyrifos-degrading bacteria from the tea-growing soil of Vietnam. For this, soil samples were collected from the 20 tea-growing areas of Vietnam. From the collected samples, three bacterial strains viz., Methylobacterium populi CNN2, Ensifer adhaerens VNN3, and Acinetobacter pittii CNN4 have been isolated by using streak plate method and identified based on 16S rRNA gene analysis. The study results showed that under laboratory conditions, E. adhaerens VNN3 had the highest CPF degradation ability and was followed by the strain M. populi CNN2. In liquid medium, CPF concentration (100 mg/L) was reduced by 95.2% and 81.4% by E.adhaerens VNN3 and M. populi CNN2, respectively, after 72 h. Further, under in-vitro conditions, the concentration of CPF was reduced from 500 mg/kg to 112 ± 1.73 (77.6%) and 197 ± 2.08 mg/kg (60.6%) by E. adhaerens VNN3 and M. populi CNN2, respectively. Based on the obtained results, it can be concluded that E. adhaerens VNN3 and M. populi CNN2 can be used for CPF-contaminated agricultural soil remediation

Data Structure Model on the Quality of Public Passenger Transport Services by Bus in Vietnam

The system of managing public passenger transport services (PPTS) by bus is a complex system, involving infrastructure, facilities, management and service communication activities between passengers and transport systems. In particular, quality information is the most important factor, providing necessary data for analysis, setting out measures to improve quality to meet the needs of passengers and meet the requirements of related parties. According to the peculiarity of the service, this study selects a database structure model to guide the process of computerizing the management of quality of PPTS by bus in urban areas in Vietnam. The results show that four database systems reflecting the quality information of the infrastructure, means of transport, transport operation and passenger service and each database system is structured by components that ensure proper implementation of QM process according to the continuous quality improvement cycle. The components of each database system are dispersed according to the scope of management to ensure the consistency for the quality management process and facilitate the collection, processing and distribution of information of related parties. Keywords: Quality, transport services, public passengers, Vietnam. DOI: 10.7176/JESD/10-10-13 Publication date:May 31st 201

Otolith morphometry and its role in determining the growth of Periophthalmus chrysospilos distributed in some coastal provinces in the Mekong Delta

This article first provided information on otolith shapes, the variation of otolith weight by sex, fish size, season, and sampling site, and the linear relationship between otolith weight and fish size. A total of 815 Periophthalmus chrysospilos specimens from Duyen Hai, Tra Vinh; Tran De, Soc Trang; Dong Hai, Bac Lieu; and Dam Doi, Ca Mau in the Mekong Delta ranged from 4.20 to 12.50 cm total length and from 1.26 to 16.88 g weight were analysed. The analysis results revealed that Ps. chrysospilos had a square otolith, deeply notched at the anterior apex and flat at the posterior apex. The weight of the left otolith showed a statistical difference according to survey factors, including gender, fish size, season, and sampling site. The otolith weight had a proportional relationship with to the total length, body weight, and head length of mudskippers (r2 =0.7~0.9), which meant that fish size could be predicted from the otolith weight and about 70-90% of the otolith weight of Ps. chrysospilos varied corresponded the growth of the fish

Development of a one-step multiplex (RT)-PCR for simultaneous detection of virus-induced respiratory disease complex in dogs in Vietnam

Canine infectious respiratory disease (CIRD) viruses, particularly canine distemper virus (CDV), canine adenovirus type 2 (CAV-2), and canine parainfluenza virus (CPIV), are the primary agents responsible for respiratory diseases in dogs. A dog can be infected with a single or multiple viruses with similar clinical signs. Although CIRD is globally prevalent in dogs, investigation into the causative viruses in Vietnam remains limited. This study aims to identify the presence of CIRD virus(es) in dogs via molecular detection. Three primer sets were newly designed and applied into (RT)-PCR reaction to detect viral genes of CDV, CAV-2, and CPIV in a commercial vaccine (Vanguard® Plus 5/L, Zoetis, USA). Both simplex and multiplex (RT)-PCR reactions using the three primer sets could detect the presence of CAV-2, CDV, and CPIV in the vaccine sample. Subsequently, the applicability of the one-step multiplex (RT)-PCR was demonstrated to test for 27 clinical nasal swab samples collected from dogs suspected of having CIRD. The results showed that our (RT)-PCR could detect CIRD virus(es) in all tested clinical samples. PCR amplicons for each representative CAV-2, CDV, and CPIV were selected for sequencing and showed high genetic similarity to respective field viruses. In conclusion, this study successfully developed a one-step multiplex (RT)-PCR reaction to detect and differentiate causative viruses of CIRD in dogs

A Research on the Quality of Public Transportation Services by Bus in Vietnam

This study was conducted to assess the status of the quality of public passenger transport services by bus in Hanoi. Data were collected from regular passengers using buses as a means of transportation in the city, including passengers standing at stations, waiting shelters and on vehicles to make trips and students of some universities who use buses as a means of transportation. We employ descriptive statistics and hierarchical analysis to learn about the topic of research. The results indicate that the quality of public transport services by buses in Hanoi, which was judged by passengers quite well. In particular, the safety level, convenience, security and hygiene is up to 70%, which was higher than the highest quality level. Quality of fast level and reliability are low. Keywords: quality of services, public passenger transport, buses, Vietnam. DOI: 10.7176/RJFA/10-13-04 Publication date:July 31st 201

FIRST RECORD OF CANTHARELLUS MINOR IN VIETNAM

This species of mushroom with orange fruiting bodies and yellow flesh grows in clumps on the forest land in the coordinates 11o56'34.45" N, 108o28'33.56" E in the pine (Pinus kesiya) forest, Da Lat City, Lam Dong Province, Vietnam. The results of analysis on the morphology, both macro- and micro-morphological characteristics of this mushroom showed that, pileus: 5-15 mm wide, infundibuliform; yellowish to orange; margin incurred, wavy-liked margin, non-striate; Pileus surface: smooth, scaleless, yellowish; Lamellae: distant, decurrent, not intervenose, concolorous to pileus; Context: concolourous to the pileus, slight sweetness, aromatic flavor; Stipe: cylindrical shape, surface smooth, concolourous to pileus, 1-2 mm diameter, 20-50 mm length; Basidiospores: 6-11.5 x 4-6.5 μm, ovoid-ellipsoid with smooth surface; Basidia: 65 x 10 μm, cornuted 4-6 spores per basidium. Phylogenetic analysis of nrLSU sequence yielded consistent topology in different taxa of Cantharellus. The phylogenetic position of XC02 was obtained and accepted at sub-generic level: subgenus Parvocantharellus. This clade was suggested to be monophyletic, and separated from other sub-generic levels. Morphologically phylogenetically distinct from the other species of clade 4, such as C. appalachiensis, C. tabernensis, C. aff. Congolensis. The highly supported monophyletic group with referent Cantharellus minor was obtained with the bootstrap value of 99, indicated that XC02 was significant closely to Cantharellus minor. Phylogenetic of nrLSU analysis revealed clades with statistical support corresponding to morphological observation, thus, XC2 was concluded as Cantharellus minor

Kinetics of cardiovascular and inflammatory biomarkers in paediatric dengue shock syndrome

Glycocalyx disruption and hyperinflammatory responses are implicated in the pathogenesis of dengue-associated vascular leak, however little is known about their association with clinical outcomes of patients with dengue shock syndrome (DSS). We investigated the association of vascular and inflammatory biomarkers with clinical outcomes and their correlations with clinical markers of vascular leakage. We performed a prospective cohort study in Viet Nam. Children ≥5 years of age with a clinical diagnosis of DSS were enrolled into this study. Blood samples were taken daily during ICU stay and 7–10 days after hospital discharge for measurements of plasma levels of Syndecan-1, Hyaluronan, Suppression of tumourigenicity 2 (ST-2), Ferritin, N-terminal pro Brain Natriuretic Peptide (NT-proBNP), and Atrial Natriuretic Peptide (ANP). The primary outcome was recurrent shock. Ninety DSS patients were enrolled. Recurrent shock occurred in 16 patients. All biomarkers, except NT-proBNP, were elevated at presentation with shock. There were no differences between compensated and decompensated DSS patients. Glycocalyx markers were positively correlated with inflammatory biomarkers, haematocrit, percentage haemoconcentration, and negatively correlated with stroke volume index. While Syndecan-1, Hyaluronan, Ferritin, and ST-2 improved with time, ANP continued to be raised at follow-up. Enrolment Syndecan-1 levels were observed to be associated with developing recurrent shock although the association did not reach the statistical significance at the P < 0.01 (OR = 1.82, 95% CI 1.07–3.35, P = 0.038). Cardiovascular and inflammatory biomarkers are elevated in DSS, correlate with clinical vascular leakage parameters and follow different kinetics over time. Syndecan-1 may have potential utility in risk stratifying DSS patients in ICU

Detection of Staphylococci from nasal samples from healthy and sick dogs in Ho Chi Minh City, Vietnam

Staphylococci are one of the most commonly found opportunistic bacteria in animals and human. The objective of this study was to determine the prevalence of Staphylococcus spp. isolated from healthy and sick dogs visiting veterinary clinics in Ho Chi Minh city, Vietnam from January 2022 to December 2022. A total of 123 nasal swab samples were collected, including 40 samples from healthy dogs and 83 samples from dogs with respiratory symptoms. Staphylococcus spp. were isolated from 47.5% of healthy animals and 60.24% of dogs showing respiratory symptoms. The study showed that isolation rates from confined, semi-confined and free-ranging dogs were 75.86%, 54.84% and 40.63%, respectively. Furthermore, the prevalence was higher in dogs over one year old compared to younger dogs (64.10% and 42.22%, respectively). Several subspecies of Staphylococci, including S. aureus, S. pseudintermedius and S. epidermidis were identified, in which S. pseudintermedius accounted for the highest proportion (48.28%). The prevalence of S. pseudintermedius also varied across different age groups. Coagulase activity of the isolated Staphylococcus strains was also determined and indicated that coagulase-positive Staphylococci constituted 80.46% of the isolates. This study represents the first report on the prevalence of Staphylococci in dogs in Vietnam and reemphasizes the zoonotic significance of Staphylococcus at the interface between dogs and humans