Histological changes following prolonged oral administration of sildenafil citrate in diabetics rats

Diabetes is a cluster of metabolic disorders reflected by abnormal hyperglycemia that causes chronic microvascular, macrovascular, and neuropathic diseases.Sildenafil citrate is widely used to dilate penile arteries, particularly in patient with erectile dysfunction which suggest that it may also dilate pulmonary arteries in patients with pulmonary hypertension. Objectives: The purpose of this study is to evaluate the effect of sildenafil citrate on heart and lung tissues of diabetic rats. Method: The study was performed using forty-two rats (42) weighing between 150-200 grams. The rats were grouped into 7 groups (A-G) of six (6) rats per 2 group. Wound area of 1.5 by 1.5 cm was created at the dorsal surface of each rat under sedation with ketamine and lignocaine. Type I diabetes was induced using a single dose of Alloxan monohydrate at dose rate of 130 mg/kg. Sildenafil citrate was administered at a dose rate of 50 mg/kg orally daily for 21 days and 10 international units of insulin was administered intraperitoneal to the control group once. At the end of 21 days lung and heart tissues were collected for histological studies. Results: The results revealed moderate thickening of interstitial demonstrated by congestion of blood vessels and oedema of the lungs. The heart muscles were swollen with loss of striation in myocardial fibers. Conclusion: Prolonged oral administration of sildenafil citrate caused appreciable pulmonary and cardiovascular damage to diabetic rats. Therefore, precautionary measures are needed when treating diabetic patients with pulmonary and cardiovascular diseases.&nbsp

Haematological changes and wound healing effects of sildenafil citrate in diabetic albino rats

This study presents baseline data on the effect of sildenafil citrate on some of the haematological and biochemical parameters in wistar rats with diabetes and wound formation. Forty two albino rats weighing between 139 and 225 g were separated at random into seven groups (A, B, C, D, E, F and G) of six rats per group. Type 1 diabetes mellitus was induced in groups A, B, C and D by a single intra-peritoneal injection of 130 mg/kg Alloxan. Rats in group A were treated with orally dose of 50 mg/kg sildenafil citrate for 21 days and rats in group B were administered 10 IU of insulin intramuscularly once and sildenafil citrate as in Group A. The rats in group C were treated with a single dose of 10 IU of insulin intramuscularly, and rats in group D with only distilled water. Groups E and F were normal rats with wounds similar to those of diabetic rats in the previous groups and each rat in group E was also treated with sildenafil citrate as in Group A and those in group F were treated with only distilled water. Group G were normal rats without wound treated with sildenafil citrate as in Group A. Blood samples were taken before (day 0) and after the administration of the sildenafil citrate on day 7, 14 and 21. The results of the study showed significant decreased in red blood cells count from 6.28 ± 0.46 × 106 to 2.81 ± 0.46 × 106 in diabetic rats during the 14 days of treatment with sildenafil citrate, however, there was an indication that continues treatment for up to 21 days reduced the blood glucose and increased the red blood cells count and this may be an indication that sildenafil citrate improves insulin mediated glucose pathways.Keywords: Diabetes, Haematological parameters, Rats, Red blood cells, Sildenafil citrat

Conversion of biomass to adsorbent: a review

Tons of biomass are produced every year including organic agricultural and forestry by-products but they are of limited value. Mostly, in the developing countries, the biomasses are considered as waste and are being burnt or thrown to liter the environment as part of teaming solid waste. Presently, there are no sustainable long-term management strategies to use biomass. The utilization of biomass to produce activated carbon is a good approach that is industrially useful and environmentally benign materials. The adsorption technique is using adsorbents in the removal of heavy metals from water therefore, biomass can be converted to the adsorbent and utilized as a waste-to-wealth commodity in water purification. In this review, the suitable process for conversion of biomass to cheap and simple means of obtaining activation carbon as adsorbent is presented. The potential uses of biomass and the conversion stages including carbonization, pyrolysis, gasification, and activation were discussed. This work depicts that the issue of solid waste utilization to solve existing issues with locally available and cheap materials is beneficial to man and the environment

Effect of sildenafil citrate on the body weight, blood glucose and white blood cell count during wound healing process in diabetic rats

Diabetes mellitus is a chronic metabolic disorder with major complication of delayed wound healing. Objectives: The purpose of this study was to evaluate the effect of sildenafil on blood glucose level, body weight and leucocytecount during wound healing in diabetic rats. Method: Forty-two Albino rats randomly divided into 7 groups of 6 rats per group were treatedorally with sildenafil (50 mg/kg) for 21 days and/or single dose of intraperitoneal injection of insulin (10 I.U.). Before treatments, diabetes was induced in groups A, B, C and D by a singleintraperitoneal injection of alloxan (130mg/kg) and a square-shaped wound measuring 1.5cm was created under anesthesia on the dorsum of rats in all groups except group G. The rats ingroup A were treated with sildenafil citrate orally at the dose rate of 50 mg/kg body weight for 21 days and the rats in group B were treated with insulin injection of 10 international units once and sildenafil citrate as in Group A. The rats in group C were treated with only insulin injection of 10 international units once and the rats in group D were treated with only distilled water. Groups E and F were non diabetic rats with wounds similar to those of diabetic rats in the previous groups and each rat in group E was also treated with sildenafil citrate as in Group A and those in group F were treated with only distilled water. The rats in Group G were normal rats without wound treated with sildenafil citrate as in Group A. Data collected were analysed using SPSS version 20. Results: The results indicated that sildenafil causes a non significant (P>0.05) decrease in the blood glucose of diabetic rats, a significant (P<0.05) increase in the leucocyte count and a significant decrease(p<0.05) in the rate of percentage decrease in bodyweight. Conclusion: Sildenafil may have the potential of reducing the rate of body weight loss in diabetic rats (with or without wound) receiving insulin treatment

Conversion of Biomass to Adsorbent: A Review

Tons of biomass are produced every year including organic agricultural and forestry by-products but they are of limited value. Mostly, in the developing countries, the biomasses are considered as waste and are being burnt or thrown to liter the environment as part of teaming solid waste. Presently, there are no sustainable long-term management strategies to use biomass. The utilization of biomass to produce activated carbon is a good approach that is industrially useful and environmentally benign materials. The adsorption technique is using adsorbents in the removal of heavy metals from water therefore, biomass can be converted to the adsorbent and utilized as a waste-to-wealth commodity in water purification. In this review, the suitable process for conversion of biomass to cheap and simple means of obtaining activation carbon as adsorbent is presented. The potential uses of biomass and the conversion stages including carbonization, pyrolysis, gasification, and activation were discussed. This work depicts that the issue of solid waste utilization to solve existing issues with locally available and cheap materials is beneficial to man and the environment