39 research outputs found

    An updated list of monogenoidea from marine fishes of Vietnam

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    In this paper, we updated the list of monogenean species from marine fishes of Vietnam. Taxonomic position of monogenean species were arranged according to the current classification system. A total of 220 monogenean species from 152 marine fish species were listed. Distribution, hosts and references of each species were given. In addition, amendations of taxonomic status of taxa were also updated. 

    Первое сообщение об Auerbachia chakravartyi (Myxosporea: Bilvavulida) из желчного пузыря Megalaspis cordyla во Вьетнаме

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    В 2017 г. в Тонкинском заливе было исследовано 20 экз. Megalaspis cordyla. Морфологическими и молекулярно-биологическими методами было установлено наличие в желчном пузыре 7 из 20 рыб (35 %) спор Auerbachia chakravartyi Narasimhamurti, Kalavati, Anuradha, Padma, 1990. Это первая находка представителей рода Auerbachia в морских рыбах Вьетнама

    Application of PCR-DGGE method for identification of nematode communities in pepper growing soil: Ứng dụng phương pháp PCR-DGGE để định danh cộng đồng tuyến trùng trong đất trồng hồ tiêu

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    Soil nematodes play an important role in indication for assessing soil environments and ecosystems. Previous studies of nematode community analyses based on molecular identification have shown to be useful for assessing soil environments. Here we applied PCR-DGGE method for molecular analysis of five soil nematode communities (designed as S1 to S5) collected from four provinces in Southeastern Vietnam (Binh Duong, Ba Ria Vung Tau, Binh Phuoc and Dong Nai) based on SSU gene. By sequencing DNA bands derived from S5 community sample, our data show 15 species containing soil nematode, other nematode and non-nematode (fungi) species. Genus Meloidogyne was found as abundant one. The genetic relationship of soil nematode species in S5 community were determined by Maximum Likelihood tree re-construction based on SSU gene. This molecular approach is applied for the first time in Vietnam for identification of soil nematode communities.Tuyến trùng đất đóng vai trò chỉ thị quan trọng trong công tác đánh giá môi trường và hệ sinh thái đất. Các nghiên cứu trước đây đã cho thấy lợi ích của việc phân tích cộng đồng tuyến trùng đất bằng định danh sinh học phân tử đối với việc đánh giá môi trường đất. Ở đây, chúng tôi ứng dụng phương pháp PCR-DGGE dựa trên gene SSU để phân tích năm (ký hiệu từ S1 đến S5) cộng đồng tuyến trùng đất thuộc các vùng trồng chuyên canh cây hồ tiêu ở miền nam Việt Nam (Bình Dương, Bà Rịa Vũng Tàu, Bình Phước và Đồng Nai). Bằng cách giải trình tự các vạch của mẫu tuyến trùng S5, kết quả cho thấy cộng đồng tuyến trùng này có 15 loài gồm nhóm tuyến trùng đất, nhóm các loại tuyến trùng khác và nhóm không phải tuyến trùng (nấm) và trong đó Meloidogyne là giống ưu thế. Mối quan hệ di truyền của các các loài tuyến trùng đất thuộc cộng đồng S5 được xác định bằng việc thiết lập cây phát sinh loài Maximum Likelihood dựa trên gene SSU. Đây là nghiên cứu đầu tiên ở Việt Nam sử dụng kỹ thuật PCR-DGGE để phân tích các cộng đồng tuyến trùng đất trồng hồ tiêu

    Redescription of three caligid species of the genus Caligus Müller, 1785 (Copepoda: Caligidae), parasites of marine fish Decapterus sp. (Perciformes: Carangidae) from Tonkin gulf, Vietnam

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    So far, parasitic copepods belonging to the family Caligidae on marine fishes in Vietnam have been poorly studied. At present, 250 copepods species belong to the genus Caligus have been registrated in the world, including 54 species recognized in waters of the South East Asia region and 11 species recorded from Vietnam. Based on the specimens of parasite copepods found on the marine fish Decapterus sp. which were collected by scientific researchers of TINRO, Vladivostok Russia from Tonkin gulf, Vietnam in 1960-1961, a re-description of three species of the genus Caligus, viz. Caligus confusus Pillai, 1961, C. constrictus Heller, 1865 and C. robustus Bassett-Smith, 1898 is given. The hosts, distribution and key to the eleven Caligus species for Vietnamese fauna are also provided herein

    Synthesis of new simplified hemiasterlin derivatives with α,β-unsaturated carbonyl moiety.

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    International audienceIn this Letter, we report a convenient and efficient method for the synthesis of new simplified derivatives of hemiasterlin in which the α,α-dimethylbenzylic moiety A is replaced by α,β-unsaturated aryl groups as Michael acceptor. Most of these derivatives have a strong cytotoxic activity on three human tumor cell lines (KB, Hep-G2 and MCF7). Analogs 17b and 17f showed a high cytotoxicity against KB and Hep-G2 cancer cell lines comparable to paclitaxel and ellipticine

    Synthesis of new bioisosteric hemiasterlin analogues with extremely high cytotoxicity

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    International audienceIn this article, we report a convenient and efficient method for the synthesis of new simplified derivativesof hemiasterlin in which the α,α-dimethylbenzylic moiety A is replaced by α,β-unsaturated aryl groupsas Michael acceptor. Most of these derivatives have a strong cytotoxic activity on three human tumorcell lines (KB, Hep-G2 and MCF7). Analogs 17b and 17f showed a high cytotoxicity against KB andHep-G2 cancer cell lines comparable to paclitaxel and ellipticine

    Light Absorption and Luminescence Properties of the Blend Poly(N-vinylcarbazole)/Poly(N-hexylthiophene)

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    We have investigated the enhancement absorption light and luminescence properties of the blend conducting polymers using poly(N-vinylcarbazole) and poly(N-hexylthiophene). The optimized material showed a broad absorption in the region of ultra violet to near infra-red and the better of luminescence ability than the pristine conducting polymers. The remarkable improvements in photoluminescences of the blends provide useful information to the application of this material in fabrication of optical – electronic devices.

    Genetic interaction between two VNTRs in the MAOA gene is associated with the nicotine dependence

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    Nicotine dependence is an addiction to tobacco products and a global public health concern that in part would be influenced by our genetics. Smokers are reported to have reduced MAOA activity, but the results from genetic associations with this gene have been inconclusive. Two functionally relevant variable number tandem repeat (VNTR) domains, termed uVNTR and dVNTR, in the MAOA gene are well characterized transcriptional regulatory elements. In the present study, we analyzed uVNTR and dVNTR polymorphisms in the MAOA gene in the Vietnamese male population of smokers and non-smokers in order to assess the association of MAOA with the nicotine dependence measured by the Fagerström Test for Nicotine Dependence (FTND). Individual analysis of VNTRs separately identified uVNTR to be associated with the F6 question of the FTND indicating the stronger addiction to nicotine. No associations were found between the dVNTR and smoking behavior. The combination of dVNTR and uVNTR, that predicts low expression of MAOA (10–3 haplotypes), was significantly associated with the higher nicotine dependence (FTND score), longer smoking duration, and more persistent smoking behavior (fewer quit attempts). In conclusion, our study confirms that low MAOA expression is genetically predictive to the higher nicotine dependence

    Short Tandem Repeats Used in Preimplantation Genetic Testing of Î’-Thalassemia: Genetic Polymorphisms For 15 Linked Loci in the Vietnamese Population

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    BACKGROUND: β-thalassemia is one of the most common monogenic diseases worldwide. Preimplantation genetic testing (PGT) of β-thalassemia is performed to avoid affected pregnancies has become increasingly popular worldwide. In which, the indirect analysis using short tandem repeat (STRs) linking with HBB gene to detect different β-globin (HBB) gene mutation is a simple, accurate, economical and also provides additional control of contamination and allele-drop-out ADO. AIM: This study established microsatellite markers for PGT of Vietnamese β-thalassemia patient. METHODS: Fifteen (15) STRs gathered from 5 populations were identified by in silico tools within 1 Mb flanking the HBB gene. The multiplex PCR reaction was optimized and performed on 106 DNA samples from at-risk families. RESULTS: After estimating, PIC values were ≥ 0.7 for all markers, with expected heterozygosity and observed heterozygosity values ranged from 0.81 to 0.92 and 0.53 to 0.86, respectively. One hundred percent of individuals had at least seven heterozygous markers and were found to be heterozygous for at least two markers on either side of the HBB gene. The STRs panel was successfully performed on one at-risk family. CONCLUSION: In general, a pentadecaplex marker (all < 1 Mb from the HBB gene) assay was constituted for β-thalassemia PGT on Vietnamese population
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