Systematic comparison of plasma EBV DNA, anti-EBV antibodies and miRNA levels for early detection and prognosis of nasopharyngeal carcinoma

Nasopharyngeal carcinoma (NPC) is originated from the epithelial cells of nasopharynx, Epstein-Barr virus (EBV)-associated and has the highest incidence and mortality rates in Southeast Asia. Late presentation is a common issue and early detection could be the key to reduce the disease burden. Sensitivity of plasma EBV DNA, an established NPC biomarker, for Stage I NPC is controversial. Most newly reported NPC biomarkers have neither been externally validated nor compared to the established ones. This causes difficulty in planning for cost-effective early detection strategies. Our study systematically evaluated six established and four new biomarkers in NPC cases, population controls and hospital controls. We showed that BamHI-W 76 bp remains the most sensitive plasma biomarker, with 96.7% (29/30), 96.7% (58/60) and 97.4% (226/232) sensitivity to detect Stage I, early stage and all NPC, respectively. Its specificity was 94.2% (113/120) against population controls and 90.4% (113/125) against hospital controls. Diagnostic accuracy of BamHI-W 121 bp and ebv-miR-BART7-3p were validated. Hsa-miR-29a-3p and hsa-miR-103a-3p were not, possibly due to lower number of advanced stage NPC cases included in this subset. Decision tree modeling suggested that combination of BamHI-W 76 bp and VCA IgA or EA IgG may increase the specificity or sensitivity to detect NPC. EBNA1 99 bp could identify NPC patients with poor prognosis in early and advanced stage NPC. Our findings provided evidence for improvement in NPC screening strategies, covering considerations of opportunistic screening, combining biomarkers to increase sensitivity or specificity and testing biomarkers from single sampled specimen to avoid logistic problems of resampling

An application for normal and critical operations in a tactical MLS system

, lacks applications demonstrating this capability. This work has developed a T-TASI system application. Through analysis, three necessary software subsystems were identified: a memory management system, a file storage system and an application-level library providing interfaces compliant with the standard C library. We describe the design, implementation, and testing of the application and the three supporting components, all of which will facilitate future application development for the T-TASI system.http://archive.org/details/anpplicationforn109455081Technology Agency author (civilian)Approved for public release; distribution is unlimited

The Conceptualisation and Development of a Space-Saving Multipurpose Table for Enhanced Ergonomic Performance

Foldable furniture is a trend of the modern furniture industry. However, apart from limitations attributed to multifunctionality and space saving characteristics, a complete design process documentation of foldable furniture is uncommon in furniture research. This study aims to develop a space-saving multipurpose table for improved ergonomic performance. Features and functions are extracted from research articles and patents for concept generation. The final concept is modelled using Autodesk Inventor Professional 2019. Mechanical simulations are done to confirm the structural integrity of the invention before prototyping and testing. The tests accounted for usage efficiency, space and usability. Using Minitab 19, the experimental data are analysed with t-tests. The survey data are analysed using Spearman’s correlation test via IBM SPSS Statistics version 21. Participants were able to complete tasks around 1.1–1.5 times faster with the proposed invention than with single-function furniture items. The amount of space occupied with the proposed invention was approximately 25–80% lesser than with the single-function furniture items placed together. The survey analysis demonstrated that there was a strong, positive and significant correlation between space saving effectiveness and ergonomic performance. Further developments to transition this invention to its commercialisation phase should be done to facilitate daily living domestic activities of society at large

Low-cost vector network analyzer for communication devices testing - brief review

A low-cost and portable one-port vector network analyzer (VNA) which covered operating frequency between 50 MHz to 1 GHz is developed for vector reflection coefficient measurement of the communication devices. A four-port reflectometry technique is implemented in the VNA module design. To collect and analyze data from the VNA, graphical user interface (GUI) is designed using LABVIEW software. In addition, open-short-load calibration technique was programmed in GUI to counterbalance systematic errors of the VNA. The performance of this designed VNA is approximately equivalent to the available commercial VNA

An integrated command and control architecture concept for unmanned systems in the year 2030

U.S. Forces require an integrated Command and Control Architecture that enables operations of a dynamic mix of manned and unmanned systems. The level of autonomous behavior correlates to: 1) the amount of trust with the reporting vehicles, and 2) the multi-spectral perspective of the observations. The intent to illuminate the architectural issues for force protection in 2030 was based on a multi-phased analytical model of High Value Unit (HVU) defense. The results showed that autonomous unmanned aerial vehicles are required to defeat high-speed incoming missiles. To evaluate the level of autonomous behavior required for an integrated combat architecture, geometric distributions were modeled to determine force positioning, based on a scenario driven Detect-to-Engage timeline. Discrete event simulation was used to schedule operations, and a datalink budget assessment of communications to determine the critical failure paths in the the integrated combat architecture. The command and control principles used in the integrated combat architecture were based on Boyd's OODA (Obseve, Orient, Decide, and Act) Loop. A conservative fleet size estimate, given the uncertainties of the coverage overlap and radar detection range, a fleet size of 35 should be anticipated given an UAV detection range of 20km and radar coverage overlap of 4 seconds.http://archive.org/details/anintegratedcomm109455244US Navy (USN) authorsApproved for public release; distribution is unlimited

High-dose methotrexate is effective for prevention of isolated CNS relapse in diffuse large B cell lymphoma

10.1038/s41408-021-00535-yBLOOD CANCER JOURNAL11

A genome wide study of copy number variation associated with nasopharyngeal carcinoma in Malaysian Chinese identifies CNVs at 11q14.3 and 6p21.3 as candidate loci

Background: Nasopharyngeal carcinoma (NPC) is a neoplasm of the epithelial lining of the nasopharynx. Despite various reports linking genomic variants to NPC predisposition, very few reports were done on copy number variations (CNV). CNV is an inherent structural variation that has been found to be involved in cancer predisposition. Methods: A discovery cohort of Malaysian Chinese descent (NPC patients, n = 140; Healthy controls, n = 256) were genotyped using Illumina® HumanOmniExpress BeadChip. PennCNV and cnvPartition calling algorithms were applied for CNV calling. Taqman CNV assays and digital PCR were used to validate CNV calls and replicate candidate copy number variant region (CNVR) associations in a follow-up Malaysian Chinese (NPC cases, n = 465; and Healthy controls, n = 677) and Malay cohort (NPC cases, n = 114; Healthy controls, n = 124). Results: Six putative CNVRs overlapping GRM5, MICA/HCP5/HCG26, LILRB3/LILRA6, DPY19L2, RNase3/RNase2 and GOLPH3 genes were jointly identified by PennCNV and cnvPartition. CNVs overlapping GRM5 and MICA/HCP5/HCG26 were subjected to further validation by Taqman CNV assays and digital PCR. Combined analysis in Malaysian Chinese cohort revealed a strong association at CNVR on chromosome 11q14.3 (Pcombined = 1.54x10-5; odds ratio (OR) = 7.27; 95% CI = 2.96–17.88) overlapping GRM5 and a suggestive association at CNVR on chromosome 6p21.3 (Pcombined = 1.29x10-3; OR = 4.21; 95% CI = 1.75–10.11) overlapping MICA/HCP5/HCG26 genes. Conclusion: Our results demonstrated the association of CNVs towards NPC susceptibility, implicating a possible role of CNVs in NPC development