704 research outputs found
Magnetic inflation and stellar mass. V. Intensification and saturation of M-dwarf absorption lines with Rossby number
In young Sun-like stars and field M-dwarf stars, chromospheric and coronal magnetic activity indicators such as Hα, X-ray, and radio emission are known to saturate with low Rossby number (Ro lesssim 0.1), defined as the ratio of rotation period to convective turnover time. The mechanism for the saturation is unclear. In this paper, we use photospheric Ti i and Ca i absorption lines in the Y band to investigate magnetic field strength in M dwarfs for Rossby numbers between 0.01 and 1.0. The equivalent widths of the lines are magnetically enhanced by photospheric spots, a global field, or a combination of the two. The equivalent widths behave qualitatively similar to the chromospheric and coronal indicators: we see increasing equivalent widths (increasing absorption) with decreasing Ro and saturation of the equivalent widths for Ro lesssim 0.1. The majority of M dwarfs in this study are fully convective. The results add to mounting evidence that the magnetic saturation mechanism occurs at or beneath the stellar photosphere.Published versio
Localization of sterols and oxysterols in mouse brain reveals distinct spatial cholesterol metabolism
Dysregulated cholesterol metabolism is implicated in a number of neurological disorders. Many sterols, including cholesterol and its precursors and metabolites, are biologically active and important for proper brain function. However, spatial cholesterol metabolism in brain and the resulting sterol distributions are poorly defined. To better understand cholesterol metabolism in situ across the complex functional regions of brain, we have developed on-tissue enzyme-assisted derivatization in combination with microliquid extraction for surface analysis and liquid chromatography-mass spectrometry to locate sterols in tissue slices (10 µm) of mouse brain. The method provides sterolomic analysis at 400-µm spot diameter with a limit of quantification of 0.01 ng/mm2. It overcomes the limitations of previous mass spectrometry imaging techniques in analysis of low-abundance and difficult-to-ionize sterol molecules, allowing isomer differentiation and structure identification. Here we demonstrate the spatial distribution and quantification of multiple sterols involved in cholesterol metabolic pathways in wild-type and cholesterol 24S-hydroxylase knockout mouse brain. The technology described provides a powerful tool for future studies of spatial cholesterol metabolism in healthy and diseased tissues
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Identification of antiviral roles for the exon-junction complex and nonsense-mediated decay in flaviviral infection.
West Nile virus (WNV) is an emerging mosquito-borne flavivirus, related to dengue virus and Zika virus. To gain insight into host pathways involved in WNV infection, we performed a systematic affinity-tag purification mass spectrometry (APMS) study to identify 259 WNV-interacting human proteins. RNA interference screening revealed 26 genes that both interact with WNV proteins and influence WNV infection. We found that WNV, dengue and Zika virus capsids interact with a conserved subset of proteins that impact infection. These include the exon-junction complex (EJC) recycling factor PYM1, which is antiviral against all three viruses. The EJC has roles in nonsense-mediated decay (NMD), and we found that both the EJC and NMD are antiviral and the EJC protein RBM8A directly binds WNV RNA. To counteract this, flavivirus infection inhibits NMD and the capsid-PYM1 interaction interferes with EJC protein function and localization. Depletion of PYM1 attenuates RBM8A binding to viral RNA, suggesting that WNV sequesters PYM1 to protect viral RNA from decay. Together, these data suggest a complex interplay between the virus and host in regulating NMD and the EJC
Laser writing of individual atomic defects in a crystal with near-unity yield
Atomic defects in wide band gap materials show great promise for development
of a new generation of quantum information technologies, but have been hampered
by the inability to produce and engineer the defects in a controlled way. The
nitrogen-vacancy (NV) color center in diamond is one of the foremost
candidates, with single defects allowing optical addressing of electron spin
and nuclear spin degrees of freedom with potential for applications in advanced
sensing and computing. Here we demonstrate a method for the deterministic
writing of individual NV centers at selected locations with high positioning
accuracy using laser processing with online fluorescence feedback. This method
provides a new tool for the fabrication of engineered materials and devices for
quantum technologies and offers insight into the diffusion dynamics of point
defects in solids.Comment: 16 pages, 8 figure
Electronic structure of the neutral silicon-vacancy center in diamond
The neutrally-charged silicon vacancy in diamond is a promising system for quantum technologies that combines high-efficiency optical spin initialization with long spin lifetimes (T2 ≈ 1 ms at 4 K) and up to 90 % of optical emission into its 946 nm zero-phonon line. However, the electronic structure of SiV0 is poorly understood, making further exploitation difficult. Performing photoluminescence spectroscopy of SiV0 under uniaxial stress, we find the previous excited electronic structure of a single 3A1u state is incorrect, and identify instead a coupled 3Eu − 3A2u system, the lower state of which has forbidden optical emission at zero stress and efficiently decreases the total emission of the defect. We propose a solution employing finite strain to define a spin-photon interface scheme using SiV0
Validation of Twelve Small Kepler Transiting Planets in the Habitable Zone
We present an investigation of twelve candidate transiting planets from
Kepler with orbital periods ranging from 34 to 207 days, selected from initial
indications that they are small and potentially in the habitable zone (HZ) of
their parent stars. Few of these objects are known. The expected Doppler
signals are too small to confirm them by demonstrating that their masses are in
the planetary regime. Here we verify their planetary nature by validating them
statistically using the BLENDER technique, which simulates large numbers of
false positives and compares the resulting light curves with the Kepler
photometry. This analysis was supplemented with new follow-up observations
(high-resolution optical and near-infrared spectroscopy, adaptive optics
imaging, and speckle interferometry), as well as an analysis of the flux
centroids. For eleven of them (KOI-0571.05, 1422.04, 1422.05, 2529.02, 3255.01,
3284.01, 4005.01, 4087.01, 4622.01, 4742.01, and 4745.01) we show that the
likelihood they are true planets is far greater than that of a false positive,
to a confidence level of 99.73% (3 sigma) or higher. For KOI-4427.01 the
confidence level is about 99.2% (2.6 sigma). With our accurate characterization
of the GKM host stars, the derived planetary radii range from 1.1 to 2.7
R_Earth. All twelve objects are confirmed to be in the HZ, and nine are small
enough to be rocky. Excluding three of them that have been previously validated
by others, our study doubles the number of known rocky planets in the HZ.
KOI-3284.01 (Kepler-438b) and KOI-4742.01 (Kepler-442b) are the planets most
similar to the Earth discovered to date when considering their size and
incident flux jointly.Comment: 27 pages in emulateapj format, including tables and figures. To
appear in The Astrophysical Journa
Subnanotesla magnetometry with a fiber-coupled diamond sensor
Nitrogen-vacancy centers (NVCs) in diamond are being explored for future quantum technologies, and in particular ensembles of NVC are the basis for sensitive magnetometers. We present a fiber-coupled NVC magnetometer with an unshielded sensitivity of (310±20)pT/√Hz in the frequency range of 10–150 Hz at room temperature. This takes advantage of low-strain 12C diamond, lenses for fiber coupling and optimization of microwave modulation frequency, modulation amplitude, and power. Fiber coupling means the sensor can be conveniently brought within 2 mm of the object under study
Prednisolone or tetracosactide depot for infantile epileptic spasms syndrome? A prospective analysis of data embedded within two randomised controlled trials
OBJECTIVE: To report a prospectively planned analysis of two randomised controlled trials with embedded comparisons of prednisolone versus tetracosactide depot for the treatment of infantile epileptic spasms syndrome (IESS). METHODS: Individual patient data from patients randomly allocated to prednisolone or tetracosactide depot were analysed from two trials (UKISS, ICISS). The comparison was embedded within trials in which some patients also received vigabatrin but only patients receiving monotherapy with randomly allocated hormonal treatments are included in this analysis. The main outcome was cessation of spasms (Days 13-14 after randomisation). Lead time to treatment and underlying aetiology were taken into account. Cessation of spasms on Days 14-42 inclusive, electroclinical response (EEG Day 14), plus developmental and epilepsy outcomes (at 14 months in UKISS and 18 months in ICISS) are also reported. Minimum treatment was prednisolone 40 mg per day for two weeks or tetracosactide depot 0·5 mg IM on alternate days for two weeks, all followed by a reducing dose of prednisolone over two weeks. RESULTS: 126 infants were included in this study. On tetracosactide depot, 47 of 62 (76%) were free of spasms on Days 13-14 compared to 43 of 64 (67%) on prednisolone (difference 9%, 95% CI -7·2% to +25·2%, chi square 1·15, p = 0·28). For Day 14-42 cessation of spasms, on tetracosactide depot, 41 of 61 (67%) were free of spasms compared to 35 of 62 (56%) on prednisolone (difference 11%, 95% CI -6·4% to +28·4%, chi square 1·51, p = 0·22). There was no significant difference in mean VABS score between infants who received prednisolone compared with those who received tetracosactide depot (74·8 (SD 18·3) versus 78·0 (SD 20·2) t = -0·91 p = 0·36). The proportion with ongoing epilepsy at the time of developmental assessment was 20 of 61 (33%) in the tetracosactide group compared with 26 out of 63 (41%) in the prednisolone group (difference 8%, 95% CI -9·2% to +25·2%, Chi [2] 0·95, p = 0·33). SIGNIFICANCE: With hormone monotherapy, either prednisolone or tetracosactide depot may be recommended for infantile epileptic spasms syndrome
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