Phylogenetic analysis of SA <i>rrn</i> OTUs (RINH01–RINH21) and IA <i>rrn</i> OTUs (T01–T10).

<p>Placement of representative sequences of the present study in clades indicated with additional reference sequences obtained from GenBank. A sequence related to the Crenarchaeota phylum (Acc. No. AF418935) was used as an outgroup. Full multiple alignment using ClustalW and a consensus tree was constructed using the Neighbor-Joining method with the Jukes-Cantor substitution model. The trees were bootstrap resampled 1000 times with branch values ≥50% shown. Scale shows 0.05 nucleotide substitutions per nucleotide position.</p

Relative distribution of methanogenic archaeal clades for different sequencing and analysis methods: A. SA <i>rrn</i>. B. IM <i>rrn</i>. C. IM <i>mcrA</i>. D. IA <i>rrn.</i>

<p>Relative distribution of methanogenic archaeal clades for different sequencing and analysis methods: A. SA <i>rrn</i>. B. IM <i>rrn</i>. C. IM <i>mcrA</i>. D. IA <i>rrn.</i></p

Multiple rarefaction collectors curves.

<p>Observed number of OTUs for different sequencing and analysis methods: A. SA <i>rrn</i>. B. IM <i>rrn</i>. C. IM <i>mcrA</i>. D. IA <i>rrn.</i></p

Bulletin municipal officiel de la ville de Paris. Délibérations des assemblées de la Ville de Paris et du Département de la Seine. Conseil municipal de Paris

11 janvier 19621962/01/11 (A81,N17)

Comparison of methanogenic archaeal diversity and depth of coverage for Sheep A and Sheep B.

<p>Shannon index (H′), Chao1 estimated number of species and Good’s statistic (C).</p><p>Comparison of methanogenic archaeal diversity and depth of coverage for Sheep A and Sheep B.</p

Additional file 4: Table S4. of The rumen microbial metagenome associated with high methane production in cattle

KEGG dataset in order of the ratio of gene abundance, high:low emitters. (XLSX 443 kb

Additional file 3: Table S3. of The rumen microbial metagenome associated with high methane production in cattle

KEGG dataset in order of average gene abundance. (XLSX 1400 kb

Additional file 6: Figure S1. of The rumen microbial metagenome associated with high methane production in cattle

KEGG pathways associated with methane metabolism. Highlighted EC gene numbers are those genes that differed significantly between high and low emitting cattle. Red – genes that had higher abundance in high emitters; blue - genes that had lower abundance in high emitters. (DOC 22 kb

Additional file 5: Table S5. of The rumen microbial metagenome associated with high methane production in cattle

Gene abundance of genes of pyruvate metabolism in low and high methane steers. (DOCX 14 kb

Sequence metadata

This file contains information on animal ID's, primer sequences, sampling periods and diets