Experimental studies on the pathogenicity of Vibrio mimicus strains isolated in Bangladesh

Vibrio mimicus, a newly described species of the genus Vibrio has been isolated from stools of 14 patients with diarrhoea. Live cells of all the 14 strains tested caused accumulation of fluid in rabbit gut loops and diarrhoea in infant rabbits. Culture filtrates of all the strains caused increased capillary permeability in rabbit skin; however, five of the filtrates resembled cholera toxin in that they gave positive reactions in rabbit loops, chinese hamster ovarian and mouse adrenal cell monolayers and GM1 ELISA tests and were neutralized by cholera antitoxin. None of the strains produced heat-stable toxin or possessed invasive capability as determined by Sereny's test. Thus, V. mimicus strains were divided into a group which produced a toxin immunobiologically similar to cholera toxin and the rest producing a heat-labile toxin unrelated to that of V. cholerae

Nuclear uncertainties in the NeNa-MgAl cycles and production of 22Na and 26Al during nova outbursts

Classical novae eject significant amounts of nuclear processed material into the interstellar medium. Among the isotopes synthesized during such explosions, two radioactive nuclei deserve a particular attention: 22Na and 26Al. In this paper, we investigate the nuclear paths leading to 22Na and 26Al production during nova outbursts by means of an implicit, hydrodynamic code that follows the course of the thermonuclear runaway from the onset of accretion up to the ejection stage. New evolutionary sequences of ONe novae have been computed, using updated nuclear reaction rates relevant to 22Na and 26Al production. Special attention is focused on the role played by nuclear uncertainties within the NeNa and MgAl cycles in the synthesis of such radioactive species. From the series of hydrodynamic models, which assume upper, recommended or lower estimates of the reaction rates, we derive limits on the production of both 22Na and 26Al. We outline a list of nuclear reactions which deserve new experimental investigations in order to reduce the wide dispersion introduced by nuclear uncertainties in the 22Na and 26Al yields.Comment: 46 pages, 4 figures. Accepted for publication in The Astrophysical Journa

Cholesterol Corrects Altered Conformation of MHC-II Protein in Leishmania donovani Infected Macrophages: Implication in Therapy

Previously we reported that Kala-azar patients show progressive decrease in serum cholesterol as a function of splenic parasite burden. Splenic macrophages (MΦ) of Leishmania donovani (LD) infected mice show decrease in membrane cholesterol, while LD infected macrophages (I-MΦ) show defective T cell stimulating ability that could be corrected by liposomal delivery of cholesterol. T helper cells recognize peptide antigen in the context of class II MHC molecule. It is known that the conformation of a large number of membrane proteins is dependent on membrane cholesterol. In this investigation we tried to understand the influence of decreased membrane cholesterol in I-MΦ on the conformation of MHC-II protein and peptide-MHC-II stability, and its bearing on the antigen specific T-cell activatio