Assessment of satisfaction with quality of stationery long-term social care system: the Stavropol Territory case

The purpose of the article is to assess satisfaction with quality of long–term stationary social care system for elderly population in the Stavropol Territory, as well as to identify problem areas in its functioning and form a set of management solutions to eliminate them. The paper analyzes the results of a sociological study having been conducted from January to July 2022. It has confirmed a high level of satisfaction with quality of the system by the elderly as well as employees of the pilot institutions of the region. The sample size of the study is 440 respondents. Such research methods as analytical method, secondary analysis, anonymous written survey, statistical methods of data processing, and graphical methods of presenting the data obtained have been used

APPLICATION OF TIME-RESOLVED FLUORESСENCE TECHNIQUE FOR STABILITY ANALYSIS OF FIREFLY LUCIFERASE LUCIOLA MINGRELICA

The characteristics of intrinsic fluorescence of firefly luciferase L. mingrelica during de-naturation with urea were studied. The dependence of both steady-state and time-resolved fluorescence parameters on the urea concentration revealed a single transition with the mid-point at 3.1±0.1M

BIOMEDICAL POTENTIAL OF ALGO-BACTERIAL SYMBIOSES

The analysis of the latest published works on the interactions between microalgae and bacteria is presented. Microalgae as a result of multimillion evolution can interact with each other and with another microorganisms. Interactions between algae and bacteria demonstrate a variety of communication from mutualism to parasitism. They can significantly affect the maintenance of vital activity, determines the direction vector, ensure the integrity of ecosystems. In modern society the attention of researches to algae-bacterial symbiosis increases as a biomass producer and as biologically active compounds. The development of green biotechnology is aimed at creating new directions for the use of algae-bacterial interactions. The analyzes materials testify to the high fundamental and applied potential of symbiosis microalgae with bacteria for biology and medicine

Spectral changes of erythrosin B luminescence upon binding to bovine serum albumin

Текст статьи не публикуется в открытом доступе в соответствии с политикой журнала

Spectral changes of erythrosin B luminescence upon binding to bovine serum albumin

Текст статьи не публикуется в открытом доступе в соответствии с политикой журнала

Structural distinctions of fast and slow bacterial luciferases revealed by phylogenetic analysis

Текст статьи не публикуется в открытом доступе в соответствии с политикой журнала.Пожалуйста, добавьте данную информацию к предоставленной версии статьи: This is a pre-copyedited, author-produced PDF of an article accepted for publication in Bioinformatics following peer review. The version of record [Structural distinctions of fast and slow bacterial luciferases revealed by phylogenetic analysis; Anna A. Deeva; Evgenia A. Temlyakova; Anatoly A. Sorokin; Elena V. Nemtseva; Valentina A. Kratasyuk; Bioinformatics 2016; doi: 10.1093/bioinformatics/btw386] is available online at: http://bioinformatics.oxfordjournals.org/cgi/content/abstract/btw386?ijkey=dKOpwyIXfFxlzEq&keytype=ref Данная версия статьи может быть выложена в открытый доступ только по прошествии 12 месяцев c момента официальной публикации на сайте Oxfordjournals

Structural distinctions of fast and slow bacterial luciferases revealed by phylogenetic analysis

Текст статьи не публикуется в открытом доступе в соответствии с политикой журнала.Пожалуйста, добавьте данную информацию к предоставленной версии статьи: This is a pre-copyedited, author-produced PDF of an article accepted for publication in Bioinformatics following peer review. The version of record [Structural distinctions of fast and slow bacterial luciferases revealed by phylogenetic analysis; Anna A. Deeva; Evgenia A. Temlyakova; Anatoly A. Sorokin; Elena V. Nemtseva; Valentina A. Kratasyuk; Bioinformatics 2016; doi: 10.1093/bioinformatics/btw386] is available online at: http://bioinformatics.oxfordjournals.org/cgi/content/abstract/btw386?ijkey=dKOpwyIXfFxlzEq&keytype=ref Данная версия статьи может быть выложена в открытый доступ только по прошествии 12 месяцев c момента официальной публикации на сайте Oxfordjournals

IDENTIFICATION AND PHYSIOLOGICAL CHARACTERIZATION OF A CONSORTIUM OF HYDROCARBON-OXIDIZING BACTERIA OF OIL AND OIL PRODUCTS

A consortium of microorganisms were isolated from TC-1 fuel form, each member of which is capable of consistently degrade hydrocarbons’ different fractions. The 5 strains of hydrocarbon-oxidizing bacteria (PSB) were identified and isolated from TS-1 jet fuel. Their physiological and biochemical features are defined. All strains exhibit positive catalase activity. It is determined that all UOB strains, producing exogenous and endogenous surfactants, are capable to growth on media with different fraction of hydrocarbons. The study of these associations allows to create effective preparations for bioremediation in the elimination of accidental spills of oil and petroleum products

Structure-Function Relationships in Temperature Effects on Bacterial Luciferases: Nothing Is Perfect

The evaluation of temperature effects on the structure and function of enzymes is necessary to understand the mechanisms underlying their adaptation to a constantly changing environment. In the current study, we investigated the influence of temperature variation on the activity, structural dynamics, thermal inactivation and denaturation of Photobacterium leiognathi and Vibrio harveyi luciferases belonging to different subfamilies, as well as the role of sucrose in maintaining the enzymes functioning and stability. We used the stopped-flow technique, differential scanning calorimetry and molecular dynamics to study the activity, inactivation rate, denaturation and structural features of the enzymes under various temperatures. It was found that P. leiognathi luciferase resembles the properties of cold-adapted enzymes with high activity in a narrow temperature range and slightly lower thermal stability than V. harveyi luciferase, which is less active, but more thermostable. Differences in activity at the studied temperatures can be associated with the peculiarities of the mobile loop conformational changes. The presence of sucrose does not provide an advantage in activity but increases the stability of the enzymes. Differential scanning calorimetry experiments showed that luciferases probably follow different denaturation schemes