колективна монографія

Кримінальний процесуальний кодекс 2012 року: ідеологія та практика правозастосування: колективна монографія / за заг. ред. Ю. П. Аленіна ; відпов. за вип. І. В. Гловюк. - Одеса : Видавничий дім «Гельветика», 2018. - 1148 с

Sour cherry strain of plum pox potyvirus (PPV): Molecular and serological evidence for a new subgroup of PPV strains

Properties of the unusual sour cherry strain of plum pox potyvirus (PPV-SoC) were investigated by sequencing its 3'-terminal 1,360 nt and examining its serological reactivity with several monoclonal antibodies (MAbs) to PPV. A significantly low degree of identity of the sequenced region has been found between PPV-SoC and other isolates of PPV. This is due to the high nucleotide divergence between the 5'-terminal region of the coat protein (CP) coding region of PPV-SoC and that of other isolates. Most nucleotide substitutions in the sequenced region are not silent and result in amino acid changes, especially near the N terminus of the CP. The unique 5' terminus of the PPV-SoC CP coding sequence has been utilized to develop a cRNA probe that hybridizes to PPV-SoC but not to other isolates/members of the D or M subgroups of PPV. The N-terminal region of the PPV-SoC CP contains the recognizable DAG motif that determines aphid transmissibility of potyviruses. Aphid transmission of PPV-SoC to herbaceous and woody hosts has been confirmed experimentally. Serological reactivity of PPV-SoC with 10 MAbs of PPV suggest that PPV-SoC represents a new serotype of PPV that does not fall into the conventional D or M serotypes/subgroups. We propose that PPV-SoC represents a new subgroup of PPV strains, termed PPV-cherry (PPV-C), and that PPV-SoC is its prototype member

Identification and characterization of the first pectin methylesterase gene discovered in the root lesion nematode Pratylenchus penetrans.

Similar to other plant-parasitic nematodes, root lesion nematodes possess an array of enzymes that are involved in the degradation of the plant cell wall. Here we report the identification of a gene encoding a cell wall-degrading enzyme, pectin methylesterase PME (EC 3.1.1.11), in the root lesion nematode Pratylenchus penetrans. Both genomic and coding sequences of the gene were cloned for this species, that included the presence of four introns which eliminated a possible contamination from bacteria. Expression of the Pp-pme gene was localized in the esophageal glands of P. penetrans as determined by in situ hybridization. Temporal expression of Pp-pme in planta was validated at early time points of infection. The possible function and activity of the gene were assessed by transient expression of Pp-pme in plants of Nicotiana benthamiana plants via a Potato virus X-based vector. To our knowledge, this is the first report on identification and characterization of a PME gene within the phylum Nematoda