Gastrophryne

Number of Pages: 2Integrative BiologyGeological Science

Gastrophryne pictiventris

Number of Pages: 2Integrative BiologyGeological Science

Gastrophryne elegans

Number of Pages: 2Integrative BiologyGeological Science

Gastrophryne olivacea

Number of Pages: 4Integrative BiologyGeological Science

Primer selection impacts specific population abundances but not community dynamics in a monthly time-series 16S rRNA gene amplicon analysis of coastal marine bacterioplankton.

Primers targeting the 16S small subunit ribosomal RNA marker gene, used to characterize bacterial and archaeal communities, have recently been re-evaluated for marine planktonic habitats. To investigate whether primer selection affects the ecological interpretation of bacterioplankton populations and community dynamics, amplicon sequencing with four primer sets targeting several hypervariable regions of the 16S rRNA gene was conducted on both mock communities constructed from cloned 16S rRNA genes and a time-series of DNA samples from the temperate coastal Santa Barbara Channel. Ecological interpretations of community structure (delineation of depth and seasonality, correlations with environmental factors) were similar across primer sets, while population dynamics varied. We observed substantial differences in relative abundances of taxa known to be poorly resolved by some primer sets, such as Thaumarchaeota and SAR11, and unexpected taxa including Roseobacter clades. Though the magnitude of relative abundances of common OTUs differed between primer sets, the relative abundances of the OTUs were nonetheless strongly correlated. We do not endorse one primer set but rather enumerate strengths and weaknesses to facilitate selection appropriate to a system or experimental goal. While 16S rRNA gene primer bias suggests caution in assessing quantitative population dynamics, community dynamics appear robust across studies using different primers

More Robust Conclusions and More Effective Applications

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Diffusion Analysis of the Emerging Market for Low Emission Vehicles

Non peer reviewedPostprin

Scholar-Librarian Collaboration in the Publication of Scholarly Materials

Recent developments in scholarly publication and the new directions being pursued in both humanities departments and libraries in the production of digital content provide opportunity for scholars and li- braries to explore new models for working together to produce and disseminate scholarly materials. We offer as a first step toward a model for publication the case of Opuscula: Short Texts of the Middle Ages and Renaissance (OSTMAR), a hybrid form of publication that leverages the university library infrastructure to create a platform for the publication of scholarly primary materials, an area of publication formerly re- served for the commercial press. This model is dependent on close collaboration between scholar and librarian, the nuances of which are outlined in this paper