Isolation of keratinophilic fungi and aerobic actinomycetes from park soils in Gorgan, North of Iran

Background: Keratinophilic fungi are a group of fungi that colonize in various keratinous substrates and degrade them to the components with low molecular weight. This study was conducted to determine the prevalence of keratinophilic fungi and aerobic Actinomycetes in soil of city parks in Gorgan. Objectives: In this study, we surveyed the city park soils of Gorgan (a northern province of Iran) to determine the identities and diversity of soil aerobic Actinomycetes, keratinophilic and non-keratinophilic fungi. Materials and Methods: A total of 244 soil samples were collected from 22 diferent parks of Gorgan, North of Iran. The samples were collected from the superfcial layer with depth not exceeding than 0-10 cm in sterile polyethylene bags. We used hair bait technique for isolation keratinophilic fungi. The colonies identifed by macroscopic and microscopic characterization after slide culturing. Actinomycetes were isolated by antibiotic dilution methods and detected by using physiological tests such as Lysozyme, Casein, Xanthine, Hypoxanthine, Gelatin, Urea Broth, and modifed acid-fast stain. Results: Totally, 75 isolates of aerobic Actinomycetes were detected that Actinomadura madurae and Nocardia asteroides were the most prevalent strains, with 14.66 and 28% prevalence respectively. Microsporum gypseum was more frequent than other keratinophilic fungi (22.96%) and Aspergillus spp. was the most species of saprophyte fungi (15.92%). Conclusions: This study showed that the collected soil from studied areas was rich of keratinophilic fungi and Actinomycetes, therefore hygiene protocol should be taken to prevent the spread of pathogenic and saprophytes fungi in the environment of susceptible person. © 2013, Ahvaz Jundishapur University of Medical Sciences

A monoclonal antibody to the gp120-CD4 complex has differential effects on HIV-induced syncytium formation and viral infectivity

A murine monoclonal antibody (MAb F-91-55) raised against the complex of soluble CD4 and human immunodeficiency virus type 1 (HIV-1) gp120 had previously been found to inhibit syncytium formation without inhibiting the interaction of CD4 with gp120, and its binding site was localized within the first two domains (D1/D2) of CD4. We investigated whether this antibody inhibited the infectivity of HIV-1 in the CD4+ T cell lines A3.01, Sup-T1 and H9. We also examined the effect of the antibody on syncytium formation between these cells and chronically infected H9 cells. Syncytium formation was found to depend critically on the incubation medium used. The effect of the MAb on HIV-1 infectivity was very limited with A3.01 and Sup-T1 cells, although it inhibited syncytium formation between A3.01 or Sup-T1 and chronically infected H9 cells. In contrast, the MAb inhibited significantly the infectivity of HIV-1 in H9 cells, but it also inhibited syncytium formation between H9 and chronically infected H9 cells to a greater extent than in the case of the other cell lines. Our results indicate that cellular systems used for syncytium assays differ in their susceptibility to inhibitory antibodies. In the A3.01 and Sup-T1 cell systems, the differences in the ability of the MAb to block viral entry or syncytium formation raise the possibility that the mechanisms of interaction of gp120/gp41 with cell membrane CD4 may be different in cell-cell and virus-cell membrane fusion

Dualisation of the D=7 Heterotic String

The dualisation and the first-order formulation of the D=7 abelian Yang-Mills supergravity which is the low energy effective limit of the D=7 fully Higssed heterotic string is discussed. The non-linear coset formulation of the scalars is enlarged to include the entire bosonic sector by introducing dual fields and by constructing the Lie superalgebra which generates the dualized coset element.Comment: 20 page

Data on the natural ventilation performance of windcatcher with anti-short-circuit device (ASCD)

This article presents the datasets which were the results of the study explained in the research paper ‘Anti-short-circuit device: a new solution for short-circuiting in windcatcher and improvement of natural ventilation performance’ [1] which introduces a new technique to reduce or prevent short-circuiting in a two-sided windcatcher and also lowers the indoor CO2 concentration and improve the ventilation distribution. Here, we provide details of the numerical modelling set-up and data collection method to facilitate reproducibility. The datasets includes indoor airflow, ventilation rates and CO2 concentration data at several points in the flow field. The CAD geometry of the windcatcher models are also included

Anti-short-circuit device: A new solution for short-circuiting in windcatcher and improvement of natural ventilation performance

Windcatcher is an effective technique for naturally ventilating a space and improving indoor air quality. A common problem for modern and traditional windcatchers is air short-circuiting. Air-short-circuiting in windcatchers occurs when the air entering through the supply channel immediately exits through the exhaust channel without circulating and mixing inside the enclosed space. Several previous works on windcatchers have observed the “short-circuiting” effect and concluded that it has a negative impact on the ventilation performance however, no work have provided a solution to eliminate this effect. The present study will address this issue by incorporating a component called the anti-short-circuiting device (ASCD) and investigating its potential to eliminate air short-circuiting in windcatchers and improve ventilation performance. Two methods were employed in this research: experimental and Computational Fluid Dynamics (CFD) study. For the experimental work, three scaled models were studied in a wind tunnel. The CFD modelling was validated using the air velocity measurements and good correlation was observed with average error below 10%. The results showed that the ASCD windcatcher with angles between 20° and 80° prevented air-short-circuiting while supplying up to 40–51 l/s per occupant, which is higher than the minimum recommendations of ASHRAE62.2 and BS5925. In addition, the windcatcher without ASCD showed 8% higher CO2 concentration in the room, indicating that the ASCD windcatcher was more effective in removing stale air out of the room. Furthermore, the average air velocity in the room at sitting height with the ASCD windcatcher was 19–28% higher than windcatcher without ASCD