A lack of freshwater reservoir effects in human radiocarbon dates in the Eneolithic to Iron Age in the Minusinsk Basin

A number of recent studies have highlighted the importance of freshwater reservoir effects (FRE) when dating human remains across large parts of Eurasia, including the Eurasian steppes. Here,we address this question in the context of the Early Bronze Age (Okunevo), Late Bronze Age (Karasuk) and Late Iron Age (Tashtyk culture) of the Minusinsk Basin, Southern Siberia. The issue is important given the large number of radiocarbon dates that have been published on human remains here, which have been used both to refine the cultural historical sequence (Svyatko et al. 2009), as well as to suggest a date of ca. 1400 BC for the appearance of millet agriculture (Svyatko et al. 2013). In these studies, it was argued that there was little or no freshwater reservoir effect to take into account, despite the likely consumption of freshwater fish. Subsequent work across the steppe raised a legitimate question concerning this assumption. Here, we present the first set of paired dates on late prehistoric humans and terrestrial fauna from the Minusinsk Basin, as well as data from modern fish for the region. The results, with one exception, show no clear evidence for a reservoir effect, with the human-fauna difference averaging −31 ± 95 14C years. Yet, dating of modern fish from the Yenisei River and its tributary Karasuk River does show a variable but significant FRE. Either this effect has changed radically over time, or the contribution of fish to human diets in the Minusinsk Basin was less than previously thought

Codon Size Reduction as the Origin of the Triplet Genetic Code

The genetic code appears to be optimized in its robustness to missense errors and frameshift errors. In addition, the genetic code is near-optimal in terms of its ability to carry information in addition to the sequences of encoded proteins. As evolution has no foresight, optimality of the modern genetic code suggests that it evolved from less optimal code variants. The length of codons in the genetic code is also optimal, as three is the minimal nucleotide combination that can encode the twenty standard amino acids. The apparent impossibility of transitions between codon sizes in a discontinuous manner during evolution has resulted in an unbending view that the genetic code was always triplet. Yet, recent experimental evidence on quadruplet decoding, as well as the discovery of organisms with ambiguous and dual decoding, suggest that the possibility of the evolution of triplet decoding from living systems with non-triplet decoding merits reconsideration and further exploration. To explore this possibility we designed a mathematical model of the evolution of primitive digital coding systems which can decode nucleotide sequences into protein sequences. These coding systems can evolve their nucleotide sequences via genetic events of Darwinian evolution, such as point-mutations. The replication rates of such coding systems depend on the accuracy of the generated protein sequences. Computer simulations based on our model show that decoding systems with codons of length greater than three spontaneously evolve into predominantly triplet decoding systems. Our findings suggest a plausible scenario for the evolution of the triplet genetic code in a continuous manner. This scenario suggests an explanation of how protein synthesis could be accomplished by means of long RNA-RNA interactions prior to the emergence of the complex decoding machinery, such as the ribosome, that is required for stabilization and discrimination of otherwise weak triplet codon-anticodon interactions

Late Quaternary climatic record from ODP Site 705 in the Northern Indian Ocean

AbstractThe uppermost 500cm sedimentary core from ODP sitelocated at the Eastern flank of Najareth bank in the Northern Indian Ocean has yielded altogether twenty four species of planktonic foraminifera. Among all these species, Globorotalia menardii has been found to be consistently dominant in the faunal assemblages from most of the samples. The δ18O measured on the tests of Globorotalia menardii from all levels help in precisely working out the sediment accumulation rates at different isotopic stages, and deciphering the change in climate in the Late Quaternary as well