The importance of subclasses of chitin synthase enzymes with myosin-like domains for the fitness of fungi

Acknowledgements TG and CF are funded by FEDER funds through the Operational Programme Competitiveness Factors – COMPETE and national funds by FCT – Foundation for Science and Technology under the strategic project UID/NEU/04539/2013. C.F. is a recipient of a postdoctoral fellowship from FCT-Fundação para a Ciência e Tecnologia (SFRH/BPD/63733/2009). NG is funded by The Wellcome Trust (080088, 086827, 075470, 099215 & 097377), the FungiBrain Marie Curie Network and the Medical Research Council (UK).Peer reviewedPostprin

Caspofungin Treatment of Aspergillus fumigatus Results in ChsG-Dependent Upregulation of Chitin Synthesis and the Formation of Chitin-Rich Microcolonies

Date of Acceptance: 23/07/2015 We thank Gillian Milne for help with electron microscopy, Sophie M. Schäfer for pilot experiments, and Emilia Mellado for strains. All authors acknowledge financial support of Gilead Sciences through Ph.D. studentships for L.A.W. and K.K.L. We also acknowledge research grants from the Wellcome Trust (080088, 086827, 075470, 099215, and 097377) and the British Society for Antimicrobial Chemotherapy.Peer reviewedPublisher PD

Fighting Novel Diseases amidst Humanitarian Crises

Humanitarian crises are becoming more prevalent and, frequently, more complex, in zones of mis-governance, lack of government presence, and even active conflict, marked by public mistrust and insecurity. The WHO and other health emergency responders lack the capacities and mandate to adequately respond. The current Ebola outbreak in an area of an active insurgency in the Democratic Republic of the Congo is just such a crisis. The State Department has banned U.S. personnel from the outbreak zone due to safety concerns, leaving the population feeling abandoned, potentially increasing the threat to the few brave health workers who remain. We need is to rethink health emergency response during complex crises and devise new strategies. We offer a blueprint for responding to health emergencies amidst complex humanitarian crises. This blueprint includes peacekeepers who have the mandate and modalities fit for the purpose of quelling a health emergency; “smart” diplomacy to negotiate with belligerents and community members to ensure health and humanitarian worker safety; and deploying all needed health, security, and diplomatic assets. We also call for international development assistance for health, including to support states in developing core public health capacities, creating inclusive health systems, and meeting other need like clean water and nutritious food. Political actors will need to assume their responsibilities if humanitarians and health workers are to carry out theirs

Dissection of the Candida albicans class I chitin synthase promoters

We acknowledge financial support from the Biotechnology and Biological Sciences Research Council (10161), Medical Research Council (New Investigator Award to C.A.M.), the European Community FUNGALWALL and SIGNALPATH initiatives and the Wellcome Trust.Peer reviewedPublisher PD

Cosmic string Y-junctions: a comparison between field theoretic and Nambu-Goto dynamics

We explore the formation of cosmic string Y-junctions when strings of two different types collide, which has recently become important since string theory can yield cosmic strings of distinct types. Using a model containing two types of local U(1) string and stable composites, we simulate the collision of two straight strings and investigate whether the dynamics matches that previously obtained using the Nambu-Goto action, which is not strictly valid close to the junction. We find that the Nambu-Goto action performs only moderately well at predicting when the collision results in the formation of a pair of Y-junctions (with a composite string connecting them). However, we find that when they do form, the late time dynamics matches those of the Nambu-Goto approximation very closely. We also see little radiative emission from the Y-junction system, which suggests that radiative decay due to bridge formation does not appear to be a means via which a cosmological network of such string would rapidly lose energy.Comment: 17 pages, 17 figures; typo correctio

A synaptic nidogen: developmental regulation and role of nidogen-2 at the neuromuscular junction

Background: The skeletal neuromuscular junction is a useful model for elucidating mechanisms that regulate synaptogenesis. Developmentally important intercellular interactions at the neuromuscular junction are mediated by the synaptic portion of a basal lamina that completely ensheaths each muscle fiber. Basal laminas in general are composed of four main types of glycosylated proteins: laminins, collagens IV, heparan sulfate proteoglycans and nidogens (entactins). The portion of the muscle fiber basal lamina that passes between the motor nerve terminal and postsynaptic membrane has been shown to bear distinct isoforms of the first three of these. For laminins and collagens IV, the proteins are deposited by the muscle; a synaptic proteoglycan, z-agrin, is deposited by the nerve. In each case, the synaptic isoform plays key roles in organizing the neuromuscular junction. Here, we analyze the fourth family, composed of nidogen-1 and -2.Results: In adult muscle, nidogen-1 is present throughout muscle fiber basal lamina, while nidogen- 2 is concentrated at synapses. Nidogen-2 is initially present throughout muscle basal lamina, but is lost from extrasynaptic regions during the first three postnatal weeks. Neuromuscular junctions in mutant mice lacking nidogen-2 appear normal at birth, but become topologically abnormal as they mature. Synaptic laminins, collagens IV and heparan sulfate proteoglycans persist in the absence of nidogen-2, suggesting the phenotype is not secondary to a general defect in the integrity of synaptic basal lamina. Further genetic studies suggest that synaptic localization of each of the four families of synaptic basal lamina components is independent of the other three.Conclusion: All four core components of the basal lamina have synaptically enriched isoforms. Together, they form a highly specialized synaptic cleft material. Individually, they play distinct roles in the formation, maturation and maintenance of the neuromuscular junction

RANGELAND ECONOMICS, ECOLOGY, AND SUSTAINABILITY: IMPLICATIONS FOR POLICY AND ECONOMIC RESEARCH

Environmental Economics and Policy, Land Economics/Use,

Cell wall stress induces alternative fungal cytokinesis and septation strategies

Peer reviewedPublisher PD