When good bugs go bad: Epidemiology and antimicrobial resistance profiles of Corynebacterium striatum, an emerging multidrug-resistant, opportunistic pathogen

ABSTRACT Infections with Corynebacterium striatum have been described in the literature over the last 2 decades, with the majority being bacteremia, central line infections, and occasionally, endocarditis. In recent years, the frequency of C. striatum infections appears to be increasing; a factor likely contributing to this is the increased ease and accuracy of the identification of Corynebacterium spp., including C. striatum , from clinical cultures. The objective of this study was to retrospectively characterize C. striatum isolates recovered from specimens submitted as part of routine patient care at a 1,250-bed, tertiary-care academic medical center. Multiple strain types were recovered, as demonstrated by repetitive-sequence-based PCR. Most of the strains of C. striatum characterized were resistant to antimicrobials commonly used to treat Gram-positive organisms, such as penicillin, ceftriaxone, meropenem, clindamycin, and tetracycline. The MIC 50 for ceftaroline was &gt;32 μg/ml. Although there are no interpretive criteria for susceptibility with telavancin, it appeared to have potent in vitro efficacy against this species, with MIC 50 and MIC 90 values of 0.064 and 0.125 μg/ml, respectively. Finally, as previously reported in case studies, we demonstrated rapid in vitro development of daptomycin resistance in 100% of the isolates tested ( n = 50), indicating that caution should be exhibited when using daptomycin for the treatment of C. striatum infections. C. striatum is an emerging, multidrug-resistant pathogen that can be associated with a variety of infection types. </jats:p

Exciton Energy Transfer in Pairs of Single-Walled Carbon Nanotubes

We studied the exciton energy transfer in pairs of semiconducting nanotubes using high-resolution optical microscopy and spectroscopy on the nanoscale. Photoluminescence from large band gap nanotubes within bundles is observed with spatially varying intensities due to distance-dependent internanotube transfer. The range of efficient energy transfer is found to be limited to a few nanometers because of competing fast nonradiative relaxation responsible for low photoluminescence quantum yield

Holidaying with the family pet: No dogs allowed!

This paper assesses the extent to which dog owners located in Brisbane, Australia, wish to holiday with their pets, and whether there is a gap between this desire and reality. The paper also examines the extent to which this demand is being catered for by the tourism accommodation sector. The need for this study reflects the increasingly significant role dogs are playing in the lives of humans, and the scale of the dog-owning population. The results suggest that, although there is a strong desire among dog owners to take holidays with their pets, the actualisation of this desire is comparatively low. A significant obstacle to the realisation of this desire appears to be a dearth of pet-friendly accommodation. This has implications for the ability of the tourism industry to benefit from this potentially lucrative market, that is, the dog-owning population

Reviewing an integrated MBA core course: Working paper series--03-16

Entering the new century, business education must respond to changes in the work environment. The traditional job as we have known it is rapidly becoming rarer, and students find themselves in an environment of higher complexity, more competition, and faster change. Employers state that students are often unprepared to meet challenges of the new work environment. In response to these influences, a team of educators at Northern Arizona University's College of Business Administration redesigned their MBA program. Based on surveys of employers, alumni and others, core courses of the MBA program became functionally integrated, cross-disciplinary, more outcome-oriented, and more rigorous. The program core was integrated in its entirety; then, components of the core were "chunked" to fit traditional time slots for administrative reasons. This paper describes design and implementation efforts for one MBA core component: Individuals, Teams, and Careers. This component presents students with an integrated view of their own strengths and weaknesses as they relate to personal, team, and career issues for students' pursuit of careers after graduation. Course content is strongly based on self-evaluations by students, then moves into group interaction strategies and skills, and finally focuses on reciprocal interactions of the person and the work environment. The multiple foci of this component include students' careers as future managers, management of their own careers and career mentorship of subordinates. Content development for the MBA program core, and thus for this component was a team effort, negotiated among the MBA redesign team. Six team members represented traditional functional areas of economics, finance, accounting, marketing, management, and computer information systems. Development and implementation of Individuals, Teams, and Careers fell to the authors as members of the MBA core faculty representing the management area. Course topics were team-taught by two management faculty members, as well as by economics, marketing, business law, and human resources faculty. In addition, guest presenters included specialists in leadership, arbitration, and a retired career manager who had been President of IBM's General Products Division. In its first iteration, the Individuals, Teams, and Careers component was presented during semester break between the first and second semesters of the MBA program. With this medial position in the core, much of the team content was based on students' retrospective views of team interaction and performance during the first half of the program. The course has since been moved to the beginning of the course sequence. In this current position, this "chunk" appears as one of two initial program components and fulfills the socialization and expectations-setting function for individual and team performance in the program, as well as addressing career management at the start of the program. This paper describes the integrated content of this MBA program component, including its multiple focus and its outcome orientation. In addition, dimensions of the team-teaching experience in this course component are examined. Finally, students' responses to this component are reported, relative to content and pedagogy. We expect other educators will benefit from seeing Northern Arizona University's response to the dramatically changing environment of the 21st century

Validity of interpretation: a user validity perspective beyond the test score

YesThis paper introduces the concept of user validity and provides a new perspective on the validity of interpretations from tests. Test interpretation is based on outputs such as test scores, profiles, reports, spread-sheets of multiple candidates’ scores, etc. The user validity perspective focuses on the interpretations a test user makes given the purpose of the test and the information provided in the test output. This innovative perspective focuses on how user validity can be extended to content, criterion and to some extent construct-related validity. It provides a basis for researching the validity of interpretations and an improved understanding of the appropriateness of different approaches to score interpretation, as well as how to design test outputs and assessments which are pragmatic and optimal

Cell wall protection by the Candida albicans class I chitin synthases

Open Access funded by Medical Research Council Acknowledgments We thank Kevin Mackenzie in the Microscopy and Histology Core Facility (Institute of Medical Sciences, University of Aberdeen), and Donna MacCallum for helpful statistical advice. This work was supported by grants from the Wellcome Trust (0868827 and 080088) including a Wellcome Trust Strategic Award (097377) and an Investigator Award to NG (101873), an MRC New Investigator Award to ML (MR/J008230/1) and a PhD scholarship awarded to KP from the Ministry of Sciences and Technology and Chiang Mai University, Thailand. Author contributions are as follows: KP constructed strains, performed the majority of the experiments, analyzed the data and contributed to the preparation of the manuscript. JA produced Fig. S1 using the data from the phosphoproteomic analysis conducted by SP and AB. NG conceived and designed experiments, analyzed data and commented on drafts of the manuscript. ML constructed strains, conceived, designed and performed experiments, analyzed data and wrote the manuscript.Peer reviewedPublisher PD

Natural Recession of the Eastern Margin of the Leofnard Salt in Western Canada

The Lloydminster area (T35-65, R15W3M-10W4M) of east-central Alberta and west-central Saskatchewan, Canada, is dissected by the north-northwest trending updip active dissolution margin, of the Devonian Leofnard Member rock salt. West of this margin, up to 150 m of rock salt is preserved; updip and to the east, the salt has mostly been leached from the rock record. The margin is up to 40 km wide and characterized by extreme local variations in net salt thickness. The dissolution of the Leofnard rock salt in the Lloydminster area has resulted in the entrapment of significant hydrocarbon accumulation. Stratigraphic traps, for example, have formed where reservoir facies were either preferentially deposited or preserved in salt-dissolution lows. Structural traps, in contrast, have formed where reservoir facies are draped across residual salt or collapse features. It has been estimated that three trillion barrels of oil (mostly of high viscosity and unrecoverable) are entrapped along the eastern dissolution margin of the Leofnard rock salt in western Canada. A record of the westward progression of the dissolutional edge of the Leofnard salt is locked in the stratigraphic column. This progression is recorded as localized interval thickening in areas where dissolution and deposition were contemporaneous. The horizontal positioning of these interval thicks as a function of their geologic age provides a time record for the positioning of the salt edge. To further explain the process of salt dissolution in the Lloydminster area, we present a suite of contour maps, geologic cross-sections, and seismic profiles. These data depict the present-day distribution of the Leofnard salt in the Lloydminster study area. They support the theses that: (1) the dissolution margin of the Leofnard rock salt originated along the Elk Point outcrop to the east of the study area during the pre-Cretaceous; and (2) the margin receded into the northeastern part of the Lloydminster study area during earliest Cretaceous or pre-Cretaceous time and migrated progressively thereafter into its current position. From the perspective of the explorationist, such information is important because it identifies prospective play areas with high potential for the formation of salt-related stratigraphic traps and/or structural traps

Evaluation of the BioFire FilmArray pneumonia panel for detection of viral and bacterial pathogens in lower respiratory tract specimens in the setting of a tertiary care academic medical center

Our objective was to evaluate the diagnostic yield and accuracy of the BioFire FilmArray pneumonia panel (BFPP) for identification of pathogens in lower respiratory tract specimens

The ultrafast ground and excited state dynamics of cis-hexatriene in cyclohexane

One- and two-color kinetics have been combined with broadband ultraviolet transient absorption spectroscopy in the 265–300 nm region to elucidate the photophysics of cis-hexatriene in cyclohexane solvent. The lowest singlet excited state, the 2 1A121A1 state, is observed to have a lifetime of 200±50 fs. The ground-state hexatriene is produced vibrationally hot. The excess vibrational energy permits ultrafast isomerization around the C–C single bonds in hexatriene. This results in a dynamic equilibrium of the three cis-hexatriene rotamers, which then relaxes multiexponentially to the room-temperature distribution in which the di-s-trans-Z-hexatriene form predominates. The peak of the mono-s-trans (cZt-HT) population is estimated to be ∼50%. Vibrational cooling results in trapping of a small amount, ∼8%, of cZt-HT that relaxes on a much longer time scale as the barrier to isomerization becomes important. An estimate of the absorption spectrum of cZt-HT is deduced from analysis of the spectral data at 50 ps. © 1997 American Institute of Physics.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/70051/2/JCPSA6-107-13-4985-1.pd