Competitive antagonism associated with blockade of neuronal uptake: effects of indoramine in rat vas deferens and aorta

UNESP Instituto de Biociências Department of PharmacologyUniversidade Federal de São Paulo (UNIFESP), Escola Paulista de Medicina (EPM) Department of PharmacologyUNIFESP, EPM, Department of PharmacologySciEL

Effects of in vitro, acute and chronic treatment with fluoxetine on the sympathetic neurotransmission of rat vas deferens

It is described that fluoxetine treatment is able to induce ejaculatory disorders. However, the exact mechanism is still not fully understood. Therefore, this study was carried out to further evaluate the anti-ejaculatory effects of fluoxetine, using different approaches (in vitro or in vivo treatments), on the sympathetic neurotransmission of the rat vas deferens. Vas deferens from male Wistar rats were used to check the in vitro effects of fluoxetine 10(-6) M, 3.10(-6) M or 10(-5) M. Animals were also acutely (20 mg/kg, i.p. 4 h or 24 h) or chronically (10 mg/kg, i.p., 30 days) treated with fluoxetine or drug-free vehicle. The vas deferens from non -treated and treated animals were isolated and mounted in an isolated organ bath for the study of the contractions induced by adrenergic agonists, tyramine, 5-HT, Ca2+ or electrical field stimulation. In vitro or acute treatment with fluoxetine decreased the contraction induced by agonists, Ca2+ or electrical field stimulation. The chronic treatment with fluoxetine decreased the contractions induced agonists, tyramine or Ca2+, but did not modify the contractions induced by electrical field stimulation. We have shown that in vitro or in vivo fluoxetine treatment is able to alter the sympathetic neurotransmission of the rat vas deferens which could be related to alterations in the calcium signalling. (C) 2016 Elsevier B.V. All rights reserved.CAPESFAPESPCNPqUniv Fed Sao Paulo, Dept Pharmacol, Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Pharmacol, Sao Paulo, BrazilWeb of Scienc