Development, characterization, efficacy and repeated dose toxicity of nanoemulsified ethanolic extract of Enicostemma littorale in Streptozotocin-induced diabetes rats.

Alginate nanocapsules of ethanolic extract of Enicostemma littorale (NEL) were prepared by emulsification, cross linking with calcium chloride and solvent removal. Based on total phenol content the loading efficiency of the nanocapsules was 89% at an optimum concentration of 2: 18 (mg ml -1) for plant extract: olive oil. The photon correlation spectroscopy (PCS) revealed that the mean particle diameter of optimized formulation was 233 nm and scanning electron microscopy (SEM) showed a spherical morphology. When subjected to Fourier transform infrared spectroscopy (FT-IR) for the compatability analysis between plant extract and sodium alginate, it revealed that the phytoconstituents were stable. The purpose of the present study was to compare the anti-diabetic activity of NEL and E.littorale (EL) in streptozoticin induced male rats. An oral dose of NEL (20 mg/kg b.w) and EL (2000 mg/kg b.w) showed a relatively similar antidiabetic effect, reducing the blood glucose, triglycerides, cholesterol, creatinine, ALT, AST, and ALP. Moreover, NEL is 100 times less than EL exhibiting better results within 10 days of treatment. These biochemical assessments were supported by rat biopsy examinations. In conclusion, the nanoemulsification method can be applied for poor water-soluble ethanolic herbal extracts to reduce the dosage and time

Genotoxicity Evaluation of Commercially Available Acid Red Dye by Comet Assay in Fish (Cyprinus Carpio)

Genotoxicity of commercially available acid red dye on fish was evaluated. Fish were exposed to various concentrations of the dye and gill processed for comet assay. Nucleoids were visually scored and categorized into various damage degrees. Significant increase (p < 0.05) in the percentage and distribution of damaged nucleoids was recorded in all dye-treated groups over control. DNA damage scores (AU) increased with exposure concentrations and dose-response was observed at higher doses. From the results it is concluded that commercially available acid red dye is potentially genotoxic to fish. The results are preliminary and further studies are warranted to acknowledge this effect. Keywords: acid red, commercial dyes, genotoxicity, comet assay

IN-VITRO OBSERVATION OF REPAGLINIDE ENGINEERED POLYMERIC NANOPARTICLES

Nanoparticles (NP) are tiny materials that have specific physicochemical properties different to bulk materials of same composition and such properties make them very attractive for commercial and medical development. The aim of this work was to produce and characterize Repaglinide (Rg) engineered Ethyl Cellulose NP by the solvent evaporation model, in an attempt to obtain a novel delivery system adequate for the treatment of diabetes. Batches were prepared with different ratios of drug and polymer in order to evaluate the influence of drug on NP properties. The absence of any chemical interaction between drug and polymer was confirmed by Fourier Transform Infra Red spectroscopy (FT-IR), Differential Scanning Calorimetry (DSC) and Thermo Gravimetric Analysis (TGA) techniques. X-Ray Diffraction pattern of formulation confirmed the superimposition of polymer and drug with lower intensity. The average diameter of NP determined by Photon Correlation Spectroscopy (PCS) was about 100nm. NP showed 86.4% Encapsulation Efficiency (EE) and 9.61% drug loading. Finally, the in-vitro release profile observed for these NP was characterized by a delayed release phase. Questions about the potential haemolytic activity of formulated nanoparticles i.e., cytotoxicity was proved by our results. Interestingly, these results suggest that nano encapsulation of the drug in biodegradable, biocompatible polymer will improve its pharmacological significance

Nanoemulsified ethanolic extract of <i style="mso-bidi-font-style:normal">Pyllanthus amarus </i>Schum & Thonn ameliorates CCl₄ induced hepatotoxicity in Wistar rats

785-794Phyllanthus amarus (PA) is commonly used in traditional medicine for hepatoprotectivity. The major limitation is that, treatment requires a large quantity of herbal extract for a longer duration. Aim of the present study was to encapsulate ethanolic plant extract for sustained release of constituents in intestine and facilitate maximum absorption. The efficacy was compared for the hepatoprotective activity of nanoencapsulated ethanolic extract of P. amarus (NPA) and PA in carbon tetrachloride (CCl4) induced hepatotoxic male rats. Based on total phenol content (TPC), the loading efficiency of nanocapsules was 89% (pH 7.0) and optimum concentration was 2:18 (mg/mL) for plant extract: olive oil. Scanning electron microscopy (SEM) showed a spherical morphology, photon correlation spectroscopy (PCS) identified mean particle diameter as 213 nm and Fourier transform infrared spectroscopy (FT-IR) revealed that the phytoconstituents were stable. An oral dose of NPA (20 mg/kg body wt.) showed a better hepatoprotective activity than PA (100 mg/kg body wt.) and also repeated dose oral toxicity proved to be safe. These biochemical assessments were supported by rat biopsy examinations. In conclusion, the nanoemulsification method may be applied for poor water-soluble ethanolic herbal extracts to reduce the dosage and time

Evaluation of wound healing potential and antimicrobial activity of ethanolic extract of Evolvulus alsinoides

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Effect of formulation variables on Rifampicin loaded alginate beads

The present work investigated the preparation of biodegradable beads with alginate polymer by ionotropic gelation method to improve the control release properties of the antibiotic rifampicin. Ionotropic gelation method was applied to prepare beads using calcium chloride (CaCl2) as cationic component and alginate as an anionic component. In this method, adding 0.5% w/v polyvinyl alcohol (PVA) to sodium alginate (3.0% w/v) and 2% w/v of polyvinyl pyrrolidone (PVP) to the CaCl2 solution were maintained to study the drug-loading and its released characteristics. The results showed that the addition of PVA and PVP significantly improved drug-loading, encapsulation efficiency and release characteristics. This demonstrates that the ionic gelation of alginate molecules offers a flexible and easily controllable process