Identification of intimin alleles in pathogenic Escherichia coli by PCR-restriction fragment length polymorphism analysis

A rapid two-step identification method based on PCR-RFLP analysis of the intimin gene was developed to differentiate specific alleles in pathogenic Escherichia coli. This technique, tested on isolates eae-positive, accurately detects eae and resolves alleles encoding the α1, α2, β, γ1, γ2/θ, κ, ɛ, ζ, and ι intimin variants

Early Cities or Large Villages? Settlement dynamics in the Trypillia group, Ukraine

This thesis investigates the development of the largest sites - megasites - in Europe during the Chalcolithic (4th millennium BC) and seeks to discuss their urban nature within the wider settlement context of the Trypillia group in modern Ukraine. The study brings together a number of different archaeological datasets and sources, including remote sensing, field survey and legacy data, into a single GIS-based interpretative framework. The assessment of the potential of these sources of information is carried out in order to establish a new field methodology, as well as a nuanced theoretical framework, for the study of the nature of megasites. Quantitative analyses are performed in order to describe trans-scalar patterns in the settlement data, and an interpretative narrative is proposed to explain these patterns and their relevance for the definition of the ‘urban’ nature of megasites. Concepts like ‘seasonality’ and ‘heterarchy’ are used to explain the development and the social organization of megasites, which are conceived as temporary gathering places where an ‘urban-like’ identity starts to develop. The results suggest that Trypillia megasites can be defined as ‘urban forms’ within their coeval settlement and social context, but in the long-term perspective of the last six millennia they are “only” large/overgrown villages. The contribution of this thesis is not only to the specific field of Trypillia archaeology, but it also provides new insights into the wider investigation of the origins of global urbanism

Gender stereotypes in children\u27s literature

This research paper focused on the creation of nine professional development sessions using critical literacy practices in order to analyze and examine gender stereotypes in children\u27s literature addressed in the classroom. This professional development grew out of an in-depth examination of the research over the past 40 years addressing gender stereotypes and gender representation in children\u27s literature, as well as, the literature on critical literacy practices and issues of social justice. The professional development sessions were designed around three major goals: to enable teachers to independently analyze children\u27s literature for gender stereotypes; to help teachers understand the four components of critical literacy; and have teachers enact critical literacy practices in the classroom in order to create a gender-fair literacy curriculum