Effects of different application methods of chitosan on growth, yield and quality of tomato (Lycopersicon esculentum Mill.)

A pot experiment was conducted to study the effect of different application methods of chitosan on growth, yield and quality of tomato (Lycopersicon esculentum Mill.). The experiment was laid out in completely randomized design (CRD) with four replications and twelve treatments combinations viz., T0= Control, T1= Soil application of chitosan (SAC) @80 ppm, T2= SAC @120 ppm, T3= Foliar spraying of chitosan (FSC) @60 ppm, T4= FSC @80 ppm, T5= FSC @100 ppm, T6= Combination of T1  and T3, T7= Combination of T1 and T4, T8= Combination of T1 and T5, T9= Combination of T2 and T3, T10= Combination of T2   and T4, and T11= Combination of T2 and T5. The study results revealed that there were significant variations among the treatments on number of leaves, numbers of flower cluster, flowering duration, fruit length and yield of tomato (L. esculentum). The highest yield of tomato (L. esculentum) was obtained from the treatment T6, while the lowest was obtained from control treatment. Vitamin-C and lycopene  content of tomato (L. esculentum) fruits varied from 2.19-4.09 and 2.38-3.58 mg 100g-1 sample, respectively. Among the major minerals, the highest amounts of Ca, Mg, Na, K, S and P were obtained from T7 (0.69%), T3 (0.58%), T8 (0.38%), T1 (0.62%), T4 (0.15%) and T6 (0.33%) treatments, respectively. Study results inferred that the treatment T4 was more effective concerning most of the growth and biochemical parameters of tomato (L. esculentum). Finally, the study concluded that foliar application of chitosan alone or in combination with soil has significant effect on growth, yield and biochemical characters of tomato (L. esculentum)

Posterior fixation including the fractured vertebra in short segment fixation of unstable thoracolumbar junction burst fractures

The aim of this study was to evaluate the efficacy of inclusion of the fractured vertebra in short segment fixation in terms of clinical and the radiological outcomes in unstable thoracolumbar junction burst fractures at a minimum of 1 year follow-up. Records of 52 patients (age: 21-50 years) with thoracolumbar burst fracture (T10L2) in Magerl Type A fractures underwent posterior pedicle screw fixation including the fractured vertebra. Clinical parameters were back pain using Visual Analogue Score (VAS) and disability using Oswestry disability index (ODI), neurological deficit (using ASIA grade) and radiologic parameters (Cobb angle, the kyphotic deformation and vertebral height) were measured before surgery and at 3, 6 and 12 months  post-operatively. The presence of screw breakage, screw pullout, peri-implant loosening, and rod breakage were considered as criteria for implant failure. The majority of fractures resulted due to falls (31 cases), and the remaining cases resulted from car accidents (21 cases). The fractured vertebral body level was L1, T12, L2, T11, and T10 in 23, 17, 6, 4 and 2 cases and achieved satisfactory clinical outcomes according to the modified Mcnab criteria 18, 25, 6 and 3 cases were considered to have excellent, good, fair, and poor outcome. The mean kyphotic angle at pre-, post-operative and final follow-up was 13.5 ± 6.3, 13.4 ± 4.3, 8.5 ± 6.  The average loss of kyphosis correction was 6.4 ± 5.2° at the final follow-up. The mean pre- and post-operative kyphotic deformation of vertebral body was 5.1 ± 3.2, 4.8 ± 2.3 and at final follow-up was 4.5 ± 4.0 (p>0.05). The mean anterior and posterior vertebral height also showed significant improvements post-operatively, which were maintained at the final follow-up. The mean ODI and VAS scores at the end of 1 year were 17.4%, 1.7 respectively. There was no case of major complication after surgery and during the follow-up period. In conclusion, reduction of unstable thoracolumbar junction burst fracture can be achieved and maintained with the use of short-segment pedicle screw fixation including the fractured vertebra, avoiding the need for anterior reconstruction.

MGMT and SPOCK2 promoter methylation in diffuse large B-Cell lymphoma: a study in two tertiary health centres in the East Coast of Malaysia

MGMT (O6-Methylguanine-DNA Methyltransferase) suppresses tumor development by removing alkyl adduct, while SPOCK2 (SPARC/Osteonectin CWCV and Kazal-like domains proteoglycan) abolishes the inhibition of membrane-type matrix metalloproteinases (MT-MMP) which leads to angiogenesis. Hence, MGMT methylation may initiate malignant cells transformation. In contrast, SPOCK2 methylation is hypothesized not to be a common event in diffuse large B-cell lymphoma (DLBCL). In this study, we examined the methylation status of MGMT and SPOCK2 in DLBCL as in Malaysia the information is extremely lacking. A total of 88 formalin-fixed paraffin-embedded tissue of patients diagnosed with DLBCL from the year 2006 to 2013 were retrieved from Hospital Universiti Sains Malaysia, Kelantan and Hospital Tengku Ampuan Afzan, Pahang. Methylation-specific polymerase chain reaction (MSP) was used to examine the methylation status of both genes. Interestingly, methylation of MGMT was detected in all the 88 DLBCL samples, whereas SPOCK2 was found to be methylated in 83 of 88 (94.3%) DLBCL cases. Our study showed a remarkably high percentage of promoter methylation of both MGMT and SPOCK2 genes. Our finding also negates initial expectation that SPOCK2 methylation would be an uncommon event in the majority of DLBCL cases. This study has shown a very high percentage of promoter methylation of MGMT and SPOCK2 in the DLBCL cases studied by MSP, using archival lymphoma tissues. Nonetheless, additional research is needed to quantitatively evaluate MGMT and SPOCK2 methylation, and to analyse gene expression and/or protein expression in order to further understand the role of MGMT and SPOCK2 methylation in the pathogenesis of DLBCL

Pyrosequencing-based quantitative identification of p16 methylation in diffuse large b-cell lymphoma at two centres in the east coast of malaysia

Introduction: Methylation of promoter region of p16 leading to gene silencing has been implicated in a wide range of malignancies including lymphomas. In diffuse large B cell lymphoma (DLBCL) particularly, a varying percentage of epigenetic inactivation of p16 promoter region was observed ranging from 16 - 54%. However, quantitative analysis of p16 promoter methylation in DLBCL has not been extensively studied in Malaysia. Objective: This study aims to quantitatively analyse p16 methylation in DLBCL samples using pyrosequencing technique. Methods: Genomic DNA was extracted from 16 formalin-fixed paraffin-embedded lymphoma tissue blocks from patients diagnosed with DLBCL. Samples were retrieved from Hospital Tengku Ampuan Afzan, Pahang and Hospital Universiti Sains Malaysia, Kelantan. Primers were designed to amplify bisulfite-treated DNA targeting p16 promoter region. Methylation status of 7 CpG sites was determined by pyrosequencing. Results: All the 16 samples studied showed promoter methylation of p16. The range of mean methylation percentage was between 18 to 81%. Conclusion: The present study has successfully measured the level of methylation of p16 in all 7 CpG sites despite the limitation in sample size. Since p16 methylation is a common event in our series of DLBCL cases, it is worth including a larger sample size in future studies to increase the chance of finding a significant correlation with clinical parameters

As You Are, So Shall You Move Your Head: A System-Level Analysis between Head Movements and Corresponding Traits and Emotions

Identifying physical traits and emotions based on system-sensed physical activities is a challenging problem in the realm of human-computer interaction. Our work contributes in this context by investigating an underlying connection between head movements and corresponding traits and emotions. To do so, we utilize a head movement measuring device called eSense, which gives acceleration and rotation of a head. Here, first, we conduct a thorough study over head movement data collected from 46 persons using eSense while inducing five different emotional states over them in isolation. Our analysis reveals several new head movement based findings, which in turn, leads us to a novel unified solution for identifying different human traits and emotions through exploiting machine learning techniques over head movement data. Our analysis confirms that the proposed solution can result in high accuracy over the collected data. Accordingly, we develop an integrated unified solution for real-time emotion and trait identification using head movement data leveraging outcomes of our analysis.Comment: 9 pages, 7 figures, NSysS 201

Molecular docking supported investigation of antioxidant, analgesic and diuretic effects of Costus speciosus rhizome

ABSTRACT. The aim of the current study was to analyze the polyphenols and determines the antioxidant, analgesic and diuretic properties of the methanolic extract of C.speciosus rhizome. DPPH and ferric reducing antioxidant power (FRAP) assays were used to determine the antioxidant activity. Acetic acid-induced writhing and formalin-induced licking experiments were used to assess the analgesic effect. The total phenolic, flavonoid and flavonol contents were found 51.73± 0.25 mg GAE/g dry weight, 3.41± 0.07mg QE/g dry weights and 44.19± 2.24 mg QE/g dry weight, respectively. The plant extract exhibited weak antioxidant activity in the DPPH and FRAP assays, with an IC50 value of 1699±62 μg/mL and an EC50 value of 125±2 μg/mL, respectively. The extract significantly reduced the number of writhes at both doses (200 and 400 mg/kg body weight) as compared to the control. The extract (400 mg/kg) also significantly reduced the percent inhibition of licking by 31.96 and 62.69% compared to the control in the early and late phase, respectively. Compared to the standard drug furosemide, the plant extract also showed a weak diuretic effect. The docking study supported the analgesic activity of rhizome extract. The potent analgesic activity of the plant extract justifies the traditional and medicinal aspects.   KEY WORDS: Costus speciosus, Analgesic activity, Diuretic effect, Molecular docking Bull. Chem. Soc. Ethiop. 2022, 36(3), 627-640.                                                                 DOI: https://dx.doi.org/10.4314/bcse.v36i3.12 &nbsp

Red cell antibody screening: a single centre experience

The Transfusion Unit of International Islamic University Medical Centre (IIUM MC) performs screening for red cell antibodies for all patients who are to be or might be transfused with red cell components. There are two types of red cell antibodies, the alloantibodies and autoantibodies. While the red cell autoantibodies are produced against self-antigen, the alloantibodies are the results of sensitization through transfusion or pregnancies. These antibodies are implicated in haemolytic transfusion reactions. This retrospective study examines the prevalence of red cell antibodies among patients of IIUM MC. Materials and Methods: All group cross matching and group screen and hold testing performed in IIUM MC from January 2017 to June 2018 were reviewed. Results: In total, samples of 3,712 patients were subjected to antibody screening using gel card. Twelve patients (0.32%) had positive antibody screening tests. The age ranged from 17 to 77 years. Nine (75%) patients were females. Four patients had positivity with Screening Cell I, one patient with Screening Cell II and III respectively. In the remaining patients (6/12) the antibody screening was positive for both Screening Cell I and II. In nine patients (0.24%) alloantibodies were identified, with six exhibiting multiple antibodies. The alloantibodies identified include anti-Le(a), anti-Le(b), anti-Kp(a), anti-C, anti-c, anti-E and anti-e. In three patients autoantibodies with no specificities were detected. Conclusion: Although the prevalence of red cell antibodies, specifically the alloantibodies is lower compared to other studies, the finding further reiterates the need to screen for unexpected red cell antibodies prior to red cell transfusion

p16 tumor suppressor gene methylation in diffuse large B cell lymphoma: a study of 88 cases at two hospitals in the East Coast of Malaysia

Introduction: p16 gene plays an important role in the normal cell cycle regulation. Methylation of p16 has been reported to be one of the epigenetic events contributing to the pathogenesis of diffuse large B-cell lymphoma (DLBCL) which occurring at varying frequency. DLBCL is an aggressive and high-grade malignancy which accounts for approximately 30% of all non-Hodgkin lymphoma cases. However, little is known regarding the epigenetic alterations of p16 gene in DLBCL cases in Malaysia. Therefore, the objective of this study was to examine the status of p16 methylation in DLBCL. Methods: A total of 88 formalin-fixed paraffin-embedded DLBCL tissues retrieved from two hospitals located in the east coast of Malaysia, namely Hospital Tengku Ampuan Afzan (HTAA) Pahang and Hospital Universiti Sains Malaysia (HUSM) Kelantan, were chosen for this study. DNA specimens were isolated and subsequently subjected to bisulfite treatment prior to methylation specific-PCR. Two pairs of primers were used to amplify methylated and unmethylated regions of p16 gene. The PCR products were then separated using agarose gel electrophoresis and visualised under UV illumination. SPSS version 12.0 was utilised to perform all statistical analysis. Result: p16 methylation was detected in 65 of 88 (74%) samples. There was a significant association between p16 methylation status and patients aged >50 years old (p=0.04). Conclusion: Our study demonstrated that methylation of p16 tumor suppressor gene in our DLBCL cases is common and significantly increased among patients aged 50 years and above. Aging is known to be an important risk factor in the development of cancers and we speculate that this might be due to the increased transformation of malignant cells in aging cell population. However, this has yet to be confirmed with further research and correlate the findings with clinicopathological parameters

Evaluation of colony-stimulating factor 1 in invasive breast carcinoma and its association with prognostic parameters

Introduction: There were 2.1 million new cases of breast cancer in 2018 worldwide.About 60-75% of breast cancers are of invasive carcinoma of no special type (NST). Recent cancer research has focused on tumour microenvironment (TME) and the crosstalk between the cell constituents within the TME. Colony-stimulating factor 1 (CSF-1) is secreted by breast cancer cells and is the primary cytokine regulating tumour-associated macrophages (TAMs) in TME which provide pro-tumorigenic milieu for tumour progression and metastasis.This study aimed to evaluate the CSF-1 gene expression in invasive breast carcinoma and its association with pathological prognostic parameters.Materials and Methods: This study was a cross-sectional study involving 128 formalin-fixed paraffin-embedded cases diagnosed as invasive breast carcinoma NST.The cancer cells were stained with anti-CSF-1 immunohistochemical antibody, and the staining intensity was evaluated and scored into low (score 0-1) and high expression (score 2-3).Results: Ninety-three cases expressed high CSF-1. High CSF-1 expression was significantly associated with tumour grade (p=0.008). High CSF-1 expression was seen in 58 (79.5%) tumours with histological grade 2 and 29 (72.5%) with histologic grade 3, respectively. High CSF-1 expression was significantly associated with positive HER-2 (40 cases (87%),p=0.007). Conclusion: This research revealed that high CSF-1 expression is associated with poor prognostic parameters. Therefore, evaluation of CSF-1 expression will be a useful prognostic marker and may serve as potential cellular target for novel treatment modality in invasive breast cancers

Epigenetic methylation status of P16, MGMT and SPOCK2 in diffuse Large B cell lymphoma

Introduction: Epigenetic methylation has been implicated in the pathogenesis of diffuse large B cell lymphoma (DLBCL). This study investigated the methylation status of p16, MGMT and SPOCK2. Aberrantly methylated p16 and MGMT have been linked to DLBCL, but not SPOCK2. p16 inhibits cyclin-dependent kinase, which results in retinoblastoma phosphorylation and blockage of cell cycle at G1 phase. MGMT removes alkyl adduct at O6-guanine, thus preventing lethal cross-links. SPOCK2, an extracellular chondroitin and heparin sulfate proteoglycans, abolishes the inhibition of membrane-type 1-matrix metalloproteinase which might enhance the angiogenesis. The absence of SPOCK2 methylation was therefore hypothesized in the majority of cases in this study. Methods: Extracted DNA from 88 formalin-fixed paraffin-embedded (FFPE) tissues of DLBCL were subjected to bisulfite conversion followed by methylation-specific PCR (MSP) analysis for p16, MGMT and SPOCK2 methylation. p16 methylation was also quantified in 16 samples through pyrosequencing assay. Results: p16 methylation was observed in 65/88 (74%) samples by MSP. Pyrosequencing detected p16 methylation in all 16 samples ranging from 18% to 81%. MGMT methylation was detected in all 88 (100%) cases. Methylated SPOCK2 was found in 83 (94.3%) samples. There was a significant association between p16 methylation status with patients above 50 years of age (p= 0.04). Conclusions: These preliminary discoveries may serve as a good platform in order to gain a comprehensive overview on the epigenetics contribution in the pathogenesis of DLBCL. Pyrosequencing is a robust tool in detecting and quantifying methylation