Investigating the Impact of Stakeholder Management on the Implementation of a Public Access Project: The Case of Smart Cape

This paper reports on a study that uses the Stakeholder Management Theory to analyse the implementation of a pilot phase of public access project, the Smart Cape Access Initiative, a Cape Town City Council e-government initiative. Data for the study was gathered through in-depth interviews with individuals who were involved, influenced and were affected by the implementation of the pilot project. The study identified the major stakeholders of the project and assessed their importance and influence on the project. Numerous interactions between the stakeholders were identified. It was further noted that no formal stakeholder management was undertaken at the identification and planning stages of the project lifecycle. In addition, results showed that there were missed opportunities for appropriate stakeholder management throughout the project. This study offers insights into agencies involved in planning and running public access projects

Unusual case of life threatening subcutaneous hemorrhage in a blunt trauma patient

AbstractIntroductionHemorrhage is the most common cause of shock in injured patients. Bleeding into the subcutaneous plane is underestimated cause of hypovolemic shock.Presentation of caseUnrestrained male driver involved in a rollover car crash. On examination, his pulse rate was 144bpm, blood pressure 80/30mmHg, and GCS was 7/15. His right pupil was dilated but reactive. Back examination revealed severe contusion with friction burns and lacerations. A Focused Assessment Sonography for Trauma (FAST) was performed. No free intraperitoneal fluid was detected. CT scan of the brain has shown right temporo-parietal subdural hematoma and extensive hematoma in the deep subcutaneous soft tissues of the back. Decompressive cranicotomy and evacuation of the subdural hematoma was performed. On the 4th postoperative day, three liters of dark brown altered blood was drained from the subcutaneous plane.DiscussionThe patient developed severe hypovolemic shock and our aim was to identify and control the source of bleeding during the resuscitation. The source of bleeding was not obvious. Severe shearing force in blunt trauma causes separation between the loose subcutaneous tissues and the underlying relatively immobile deep fascia. This is known as post-traumatic closed degloving injury. To our knowledge this is the first reported case in the English Literature with severe subcutaneous hemorrhage in blunt trauma patients without any previous medical disease.ConclusionBleeding into the subcutaneous plane in closed degloving injury can cause severe hypovolemic shock. It is important for the clinicians managing trauma patients to be aware this serious injury

The BARD1 C-Terminal Domain Structure and Interactions with Polyadenylation Factor CstF-50†

The BARD1 N-terminal RING domain binds BRCA1 while the BARD1 C-terminal ankyrin and tandem BRCT repeat domains bind CstF-50 to modulate mRNA processing and RNAP II stability in response to DNA damage. Here we characterize the BARD1 structural biochemistry responsible for CstF- 50 binding. The crystal structure of the BARD1 BRCT domain uncovers a degenerate phosphopeptide binding pocket lacking the key arginine required for phosphopeptide interactions in other BRCT proteins.Small angle X-ray scattering together with limited proteolysis results indicates that ankyrin and BRCT domains are linked by a flexible tether and do not adopt a fixed orientation relative to one another. Protein pull-down experiments utilizing a series of purified BARD1 deletion mutants indicate that interactions between the CstF-50 WD-40 domain and BARD1 involve the ankyrin-BRCT linker but do not require ankyrin or BRCT domains. The structural plasticity imparted by the ANK-BRCT linker helps to explain the regulated assembly of different protein BARD1 complexes with distinct functions in DNA damage signaling including BARD1-dependent induction of apoptosis plus p53 stabilization and interactions. BARD1 architecture and plasticity imparted by the ANK-BRCT linker are suitable to allow the BARD1 C-terminus to act as a hub with multiple binding sites to integrate diverse DNA damage signals directly to RNA polymerase

Paleothermometric fluctuations and ornamentation pattern of Ostracoda species from a short core, off Ongole, Bay of Bengal, South-east Indian coast

819-824An investigation on the elemental chemistry and its fluctuation in Ostracoda carapace to render the environmental traits from off Ongole, Andhra Pradesh is outlined in the paper. Paleothermometric elucidation of the environment using elements Ca, Mg, As, Nb, Sb, Al, Si, Cl, K and Na and the ornamentation pattern exhibited by Ostracoda are identified and ventilated. A short core of length 20 cm was collected from the study area using a multicorer during the ORV Sagar Kanya cruise (SK-308 Leg 1) fieldwork, at a water depth of 47 m. Ostracoda separated from the sediment matrix were subjected to standard micro-paleontological procedures. The elemental weight percentages reveal that the distribution of the elements in the carapace for different species is not the same and fluctuates along the downcore. The occurrence of Niobium in the offshore sediments is observed to be of terrestrial influence, which is derived from granitic and pegmatite rocks of the adjoining coastal terrain. A traceable quantity of Antimony and Arsenic is identified in the carapace of the certain Ostracoda. Mg/Ca ratio in carapace indicates that an endurance of mild temperature fluctuation to the downcore. Smooth, spinose, pitted, punctate and reticulate ornamentation are found occurring in Ostracoda carapace which reflects on the depositional environment and granulometry

Paleothermometric fluctuations and ornamentation pattern of Ostracoda species from a short core, off Ongole, Bay of Bengal, South-east Indian coast

An investigation on the elemental chemistry and its fluctuation in Ostracoda carapace to render the environmental traits from off Ongole, Andhra Pradesh is outlined in the paper. Paleothermometric elucidation of the environment using elements Ca, Mg, As, Nb, Sb, Al, Si, Cl, K and Na and the ornamentation pattern exhibited by Ostracoda are identified and ventilated. A short core of length 20 cm was collected from the study area using a multicorer during the ORV Sagar Kanya cruise (SK-308 Leg 1) fieldwork, at a water depth of 47 m. Ostracoda separated from the sediment matrix were subjected to standard micro-paleontological procedures. The elemental weight percentages reveal that the distribution of the elements in the carapace for different species is not the same and fluctuates along the downcore. The occurrence of Niobium in the offshore sediments is observed to be of terrestrial influence, which is derived from granitic and pegmatite rocks of the adjoining coastal terrain. A traceable quantity of Antimony and Arsenic is identified in the carapace of the certain Ostracoda. Mg/Ca ratio in carapace indicates that an endurance of mild temperature fluctuation to the downcore. Smooth, spinose, pitted, punctate and reticulate ornamentation are found occurring in Ostracoda carapace which reflects on the depositional environment and granulometry

Sanguinarine Induces Apoptosis in Papillary Thyroid Cancer Cells via Generation of Reactive Oxygen Species.

Sanguinarine (SNG), a natural compound with an array of pharmacological activities, has promising therapeutic potential against a number of pathological conditions, including malignancies. In the present study, we have investigated the antiproliferative potential of SNG against two well-characterized papillary thyroid cancer (PTC) cell lines, BCPAP and TPC-1. SNG significantly inhibited cell proliferation of PTC cells in a dose and time-dependent manner. Western blot analysis revealed that SNG markedly attenuated deregulated expression of p-STAT3, without affecting total STAT3, and inhibited growth of PTC via activation of apoptotic and autophagy signaling cascade, as SNG treatment of PTC cells led to the activation of caspase-3 and caspase-8; cleavage of PARP and activation of autophagy markers. Further, SNG-mediated anticancer effects in PTC cells involved the generation of reactive oxygen species (ROS) as N-acetyl cysteine (NAC), an inhibitor of ROS, prevented SNG-mediated antiproliferative, apoptosis and autophagy inducing action. Interestingly, SNG also sensitized PTC cells to chemotherapeutic drug cisplatin, which was inhibited by NAC. Finally, SNG suppressed the growth of PTC thyrospheres and downregulated stemness markers ALDH2 and SOX2. Altogether, the findings of the current study suggest that SNG has anticancer potential against PTC cells as well its derived cancer stem-like cells, most likely via inactivation of STAT3 and its associated signaling molecules

Umbilical endosalpingiosis: a case report

<p>Abstract</p> <p>Introduction</p> <p>Endosalpingiosis describes the ectopic growth of Fallopian tube epithelium. Pathology confirms the presence of a tube-like epithelium containing three types of cells: ciliated, columnar cells; non-ciliated, columnar secretory mucous cells; and intercalary cells.</p> <p>We report the case of a woman with umbilical endosalpingiosis and examine the nature and characteristics of cutaneous endosalpingiosis by reviewing and combining the other four cases existing in the international literature.</p> <p>Case presentation</p> <p>A 50-year-old Caucasian, Greek woman presented with a pale brown nodule in her umbilicus. The nodule was asymptomatic, with no cyclical discomfort or variation in size. Her personal medical, surgical and gynecologic history was uneventful. An excision within healthy margins was performed under local anesthesia. A cystic formation measuring 2.7×1.7×1 cm was removed. Histological examination confirmed umbilical endosalpingiosis.</p> <p>Conclusions</p> <p>Umbilical endosalpingiosis is a very rare manifestation of the non-neoplasmatic disorders of the Müllerian system. It appears with cyclic symptoms of pain and swelling of the umbilicus, but not always. The disease is diagnosed using pathologic findings and surgical excision is the definitive treatment.</p