Effects of Manually Processed Bio-pesticides on Crop Production and Pest Managements in Okra (Abelmoschus Esculentus (L.) Moench)

Efficiency of different botanical extracts were tested to find out optimal concentration of extracts along with the production rate. Botanical extracts were, Neem leaf extracts (3ml/l,5ml/l & 7ml/l),Garlic bulb extracts (5ml/l,10ml/l & 15 ml/L) & Papaya leaves extracts ((5ml/l,10ml/l & 15 ml/L), among them, the production of fruit only compromised while treating with papaya leaves extracts, while Neem leaves extracts and Garlic bulb extracts were outstanding. Higher concentrations of biopesticides were effective rather than the lower concentrations to inhibit the pests. In all cases, Neem leaf extracts were outstanding in production manner as well as pest management systems. Keywords: Okra, Biopesticides & Pest management

Study of Shoot Multiplication of Strawberry (Fragaria ananassa)

Effects of different hormonal concentration on multiple shoot formation of RABI Strawberry-3 were studied. Where the explants were runner tips that cultured on MS basal medium supplemented with 6-Benzyl Adenine and Kinetin. The maximum percentage 80% of shoot was obtained from Benzyl Adenine concentrations of 0.5 mg/l and 1.0 mg/l whereas Kinetin concentrations were 1.5 mg/l and 2.0 mg/l separately. More promising result was found by combining two impressive concentrations of each hormone. When MS basal media was supplemented with Kinetin 2.0mg/l + Benzyl Adenin A 0.5 mg/l, Kinetin 1.5 mg/l + Benzyl Adenine 0.5 mg/l and Kinetin 1.5 mg/l + Benzyl Adenine 1.0 mg/l respectively then 100% shoots were obtained. DOI: http://dx.doi.org/10.3329/ijarit.v1i1-2.13936 Int. J. Agril. Res. Innov. & Tech. 1 (1&2): 69-72, December, 201

An Efficient Irrigation System for Plasticulture of Strawberry in Bangladesh

Plasticulture technique is a commonly used technique for advanced strawberry production. Due to high cost and tough maintenance, this technique is not practiced commercially in Bangladesh. We have found that, if the irrigation system can be switched to a cheaper one with high efficiency, then the cost can be minimized. We applied Micro-drip mediated irrigation system to minimize the cost. Our proposed modification of plasticulture using micro-drip irrigation system was outstanding in all our concerned horticultural parameters like height of the plants, number of leaves, north-south spreading, and east-west spreading of the plants. Paired Samples analysis between conventional and plasticulture procedure, test of significance for the pairs of mean, analysis of variance and LSD test at 5% level of significance were done for statistical analysis to conclude the  result. The statistical analysis has also shown that the plasticulture method proposed by us was highly significant over the conventional technique and practical for the rural farmer of Bangladesh. It reduces the production costs, improves fruit quality and yield, and reduces the need for chemical pesticides. Keywords: Micro drip irrigation, Soil solarization, Mulching, and Fruit Qualit

An efficient callus initiation and direct regeneration of Stevia rebaudiana

The experiment was carried out to establish an efficient callus induction system of Stevia rebaudiana from a variety of explants as well as direct regeneration from nodes. Callus initiation was done to extract secondary metabolites. However direct regeneration is essential for rapid large-scale clonal propagation. Among leaf, node and inter-nodal explants, the best callus initiation performance was observed by nodes. MS medium fortified with α-naphthalene acetic acid (NAA) 2.0 mg/L + 6-benzyladenine (BA) 2.0 mg/L showed the highest (93.33 ± 6.67%) callus induction by nodal explants. Though inter-nodal explants showed a moderate response (73.33 ± 6.67%) for callus induction in MS medium complemented with NAA 3.0 mg/L + BA 1.0 mg/L, nodal explants showed higher response (86.67 ± 13.33%) than inter-nodal explants in that nutritional environment. Leaf explants always showed very poor callus. However, the best direct regenerating medium was MS medium + BA 1.0 mg/L for multiple shoot proliferation and then transferring those shoots to ½ MS medium + NAA 1.0 mg/L for root formation.Key words: In vitro, Stevia rebaudiana, 2,4-D, α-naphthalene acetic acid (NAA), 6-benzyladenine (BA), plant growth regulators

Development of Efficient Callus Initiation of Malta (Citrus sinensis) Through Tissue Culture

The effects of different hormonal concentration on shoot formation and callus induction were studied on BARI Malta-1 (Citrus sinensis). Seeds containing seed coat and without seed coat was treated by Murashige and Skoog (MS) media supplemented with 6-benzyl adenine (BA) and Kinetin (KIN). Removal of seed coat showed an early response for shoot formation. The highest (70%) shoot formation was obtained from seeds without seed coat treated with MS basal media + BA 1.0 mg/l while KIN showed no response for shoot formation in any supplemented concentration. However, in case of callus induction internodes and apical shoot tips were used as explants and 2, 4-dichlorophenoxy acetic acid (2, 4-D) was used as callus inducing hormone. MS basal media supplemented with 2, 4-D, 2.0 mg/l showed highest (68%) callus induction. DOI: http://dx.doi.org/10.3329/ijarit.v1i1-2.13935 Int. J. Agril. Res. Innov. & Tech. 1 (1&2): 64-68, December, 201

Study of Shoot Multiplication of Strawberry (Fragaria ananassa)

Effects of different hormonal concentration on multiple shoot formation of RABI Strawberry-3 were studied. Where the explants were runner tips that cultured on MS basal medium supplemented with 6-Benzyl Adenine and Kinetin. The maximum percentage 80% of shoot was obtained from Benzyl Adenine concentrations of 0.5 mg/l and 1.0 mg/l whereas Kinetin concentrations were 1.5 mg/l and 2.0 mg/l separately. More promising result was found by combining two impressive concentrations of each hormone. When MS basal media was supplemented with Kinetin 2.0mg/l + Benzyl Adenin A 0.5 mg/l, Kinetin 1.5 mg/l + Benzyl Adenine 0.5 mg/l and Kinetin 1.5 mg/l + Benzyl Adenine 1.0 mg/l respectively then 100% shoots were obtained. DOI: http://dx.doi.org/10.3329/ijarit.v1i1-2.13936 Int. J. Agril. Res. Innov. & Tech. 1 (1&2): 69-72, December, 201

STUDY OF SHOOT MULTIPLICATION OF STRAWBERRY (Fragaria ananassa)

Effects of different hormonal concentration on multiple shoot formation of RABI Strawberry-3 were studied. Where the explants were runner tips that cultured on MS basal medium supplemented with 6-Benzyl Adenine and Kinetin. The maximum percentage 80% of shoot was obtained from Benzyl Adenine concentrations of 0.5 mg/l and 1.0 mg/l whereas Kinetin concentrations were 1.5 mg/l and 2.0 mg/l separately. More promising result was found by combining two impressive concentrations of each hormone. When MS basal media was supplemented with Kinetin 2.0mg/l + Benzyl Adenin A 0.5 mg/l, Kinetin 1.5 mg/l + Benzyl Adenine 0.5 mg/l and Kinetin 1.5 mg/l + Benzyl Adenine 1.0 mg/l respectively then 100% shoots were obtained

DEVELOPMENT OF EFFICIENT CALLUS INITIATION OF MALTA (Citrus sinensis) THROUGH TISSUE CULTURE

The effects of different hormonal concentration on shoot formation and callus induction were studied on BARI Malta-1 (Citrus sinensis). Seeds containing seed coat and without seed coat was treated by Murashige and Skoog (MS) media supplemented with 6-benzyl adenine (BA) and Kinetin (KIN). Removal of seed coat showed an early response for shoot formation. The highest (70%) shoot formation was obtained from seeds without seed coat treated with MS basal media + BA 1.0 mg/l while KIN showed no response for shoot formation in any supplemented concentration. However, in case of callus induction internodes and apical shoot tips were used as explants and 2, 4-dichlorophenoxy acetic acid (2, 4-D) was used as callus inducing hormone. MS basal media supplemented with 2, 4-D, 2.0 mg/l showed highest (68%) callus induction