Larval rearing and production of spat of the oyster Crassostrea madrasensis (Preston) in an Experimental hatchery

The Indian backwater oyster, Crassostrea madrasensis, was spawned in the laboratory at Tuticorin. The larvae were reared and the spat settled in 15-20 days. Two species of jihytoflagellates, Isochrysis galbana and Pavlova sp. were isolated from the natural environment, cultured in mass scale and given as food throughout the larval period. Early stages of the development of fertilized egg, successive stages of developing larvae, and metamorphosis to the spat have been described. The methodology of the production of cultchless spat and rearing the post-set oysters are discussed

Purification of farm grown oysters

The purification of farm grown oysters by scientific methods is essential before they are marketed. Oyster farming is a new venture in India and the technique has recently been perfected by the Central Marine Fisheries Research Institute. The most important task is to perfect the depuration process for which there is no standard method. The present paper gives a satisfactory plan for small scale purification of harvested oysters before they are marketed for safe human consumption

Induced spawning and larval rearing of Crassostrea madrasensis (Preston) in the laboratory

The technology of culturing the edible oyster Crassostrea madrasensis by rack and tray method has been developed. Investigations on induced spawning and rearing of the oyster lead to produce spat of Crassostrea madrasensis on a large scale for the first time in India.The spawning of oysters were done in suitable conditions and the fertilised eggs undergone larval rearing,each stage is examined carefully and discussed, to complete the development within 19 days

Molecular Characterization of the Gastrula in the Turtle Emys orbicularis: An Evolutionary Perspective on Gastrulation

Due to the presence of a blastopore as in amphibians, the turtle has been suggested to exemplify a transition form from an amphibian- to an avian-type gastrulation pattern. In order to test this hypothesis and gain insight into the emergence of the unique characteristics of amniotes during gastrulation, we have performed the first molecular characterization of the gastrula in a reptile, the turtle Emys orbicularis. The study of Brachyury, Lim1, Otx2 and Otx5 expression patterns points to a highly conserved dynamic of expression with amniote model organisms and makes it possible to identify the site of mesoderm internalization, which is a long-standing issue in reptiles. Analysis of Brachyury expression also highlights the presence of two distinct phases, less easily recognizable in model organisms and respectively characterized by an early ring-shaped and a later bilateral symmetrical territory. Systematic comparisons with tetrapod model organisms lead to new insights into the relationships of the blastopore/blastoporal plate system shared by all reptiles, with the blastopore of amphibians and the primitive streak of birds and mammals. The biphasic Brachyury expression pattern is also consistent with recent models of emergence of bilateral symmetry, which raises the question of its evolutionary significance

Production of oyster seed in a hatchery system

Although oyster culture dates back to First century B.C., the development of hatchery techniques for the production of oyster seed on a year round basis is a recent innovation. Since early 1950s attempts for large scale production of oyster seed have been initiated. Loosanoff and Davis (1952), Dupuy et al. (1977) and AQUACOP (1977) have successfully produced seed of the oysters Ostrea edulis, Crassostrea virginica and Crassostrea gigas. Nayar et al. (1984) have successfully accomplished the production of seed of C. madrasensis oh a large scale at the molluscan hatchery laboratory of Central Marine Fisheries Research Institute at Tuticorin. At this hatchery cultched as well as free or cultchless spat are produced. The production of oyster seed by hatchery techniques is accomplished in six phases of operations viz., (1) Conditioning adult oysters for maturation of gonads (2) Induced spawning (3) Larval rearing (4) Culture of algal food (5) Preparation of spat collectors and (6) Setting of spat. These six functions although interrelated are independent phases of operation and easy to follow and implement

Oligodendrocyte dynamics dictate cognitive performance outcomes of working memory training in mice

Previous work has shown that motor skill learning stimulates and requires generation of myelinating oligodendrocytes (OLs) from their precursor cells (OLPs) in the brains of adult mice. In the present study we ask whether OL production is also required for non-motor learning and cognition, using T-maze and radial-arm-maze tasks that tax spatial working memory. We find that maze training stimulates OLP proliferation and OL production in the medial prefrontal cortex (mPFC), anterior corpus callosum (genu), dorsal thalamus and hippocampal formation of adult male mice; myelin sheath formation is also stimulated in the genu. Genetic blockade of OL differentiation and neo-myelination in Myrf conditional-knockout mice strongly impairs training-induced improvements in maze performance. We find a strong positive correlation between the performance of individual wild type mice and the scale of OLP proliferation and OL generation during training, but not with the number or intensity of c-Fos+ neurons in their mPFC, underscoring the important role played by OL lineage cells in cognitive processing

Intraductal fully covered self-expanding metal stents in the management of post-liver transplant anastomotic strictures: a UK wide experience.

Background: Fully covered intraductal self-expanding metal stents (IDSEMS) have been well described in the management of post-liver transplant (LT) anastomotic strictures (ASs). Their antimigration waists and intraductal nature make them suited for deployment across the biliary anastomosis. Objectives: We conducted a multicentre study to analyse their use and efficacy in the management of AS. Design: This was a retrospective, multicentre observational study across nine tertiary centres in the United Kingdom. Methods: Consecutive patients who underwent endoscopic retrograde cholangiopancreatography with IDSEMS insertion were analysed retrospectively. Recorded variables included patient demographics, procedural characteristics, response to therapy and follow-up data. Results: In all, 162 patients (100 males, 62%) underwent 176 episodes of IDSEMS insertion for AS. Aetiology of liver disease in this cohort included hepatocellular carcinoma (n = 35, 22%), followed by alcohol-related liver disease (n = 29, 18%), non-alcoholic steatohepatitis (n = 20, 12%), primary biliary cholangitis (n = 15, 9%), acute liver failure (n = 13, 8%), viral hepatitis (n = 13, 8%) and autoimmune hepatitis (n = 12, 7%). Early AS occurred in 25 (15%) cases, delayed in 32 (20%) cases and late in 95 (59%) cases. Age at transplant was 54 years (range, 12-74), and stent duration was 15 weeks (range, 3 days-78 weeks). In total, 131 (81%) had complete resolution of stricture at endoscopic re-evaluation. Stricture recurrence was observed in 13 (10%) cases, with a median of 19 weeks (range, 4-88 weeks) after stent removal. At removal, there were 21 (12%) adverse events, 5 (3%) episodes of cholangitis and 2 (1%) of pancreatitis. In 11 (6%) cases, the removal wires unravelled, and 3 (2%) stents migrated. All were removed endoscopically. Conclusion: IDSEMS appears to be safe and highly efficacious in the management of post-LT AS, with low rates of AS recurrence