Distinct mechanisms of Drosophila CRYPTOCHROME-mediated light-evoked membrane depolarization and in vivo clock resetting.

Drosophila CRYPTOCHROME (dCRY) mediates electrophysiological depolarization and circadian clock resetting in response to blue or ultraviolet (UV) light. These light-evoked biological responses operate at different timescales and possibly through different mechanisms. Whether electron transfer down a conserved chain of tryptophan residues underlies biological responses following dCRY light activation has been controversial. To examine these issues in in vivo and in ex vivo whole-brain preparations, we generated transgenic flies expressing tryptophan mutant dCRYs in the conserved electron transfer chain and then measured neuronal electrophysiological phototransduction and behavioral responses to light. Electrophysiological-evoked potential analysis shows that dCRY mediates UV and blue-light-evoked depolarizations that are long lasting, persisting for nearly a minute. Surprisingly, dCRY appears to mediate red-light-evoked depolarization in wild-type flies, absent in both cry-null flies, and following acute treatment with the flavin-specific inhibitor diphenyleneiodonium in wild-type flies. This suggests a previously unsuspected functional signaling role for a neutral semiquinone flavin state (FADH•) for dCRY. The W420 tryptophan residue located closest to the FAD-dCRY interaction site is critical for blue- and UV-light-evoked electrophysiological responses, while other tryptophan residues within electron transfer distance to W420 do not appear to be required for light-evoked electrophysiological responses. Mutation of the dCRY tryptophan residue W342, more distant from the FAD interaction site, mimics the cry-null behavioral light response to constant light exposure. These data indicate that light-evoked dCRY electrical depolarization and clock resetting are mediated by distinct mechanisms

Linear stability analysis of capillary instabilities for concentric cylindrical shells

Motivated by complex multi-fluid geometries currently being explored in fibre-device manufacturing, we study capillary instabilities in concentric cylindrical flows of $N$ fluids with arbitrary viscosities, thicknesses, densities, and surface tensions in both the Stokes regime and for the full Navier--Stokes problem. Generalizing previous work by Tomotika (N=2), Stone & Brenner (N=3, equal viscosities) and others, we present a full linear stability analysis of the growth modes and rates, reducing the system to a linear generalized eigenproblem in the Stokes case. Furthermore, we demonstrate by Plateau-style geometrical arguments that only axisymmetric instabilities need be considered. We show that the N=3 case is already sufficient to obtain several interesting phenomena: limiting cases of thin shells or low shell viscosity that reduce to N=2 problems, and a system with competing breakup processes at very different length scales. The latter is demonstrated with full 3-dimensional Stokes-flow simulations. Many $N > 3$ cases remain to be explored, and as a first step we discuss two illustrative $N \to \infty$ cases, an alternating-layer structure and a geometry with a continuously varying viscosity

Nutritive value and physical characteristics of Xaraes palisadegrass as affected by grazing strategy

The aim of this study was to ascertain whether the defoliation frequency based on a fixed rest period would generate variable sward structural and physiological conditions at each subsequent grazing event. The relative importance of the physiological age was established in comparison with the chronological age in the determination of the forage nutritive value of Xaraes palisadegrass [Brachiaria brizantha (Hochst ex A. RICH.) STAPF. cv. Xaraes]. Two grazing frequencies were defined by light interception (LI) at initiation of grazing (95% LI - ""target grazing"" [TG] or 100% LI - ""delayed grazing"" [DG]) and one based on chronological time, grazing every 28 days (28-d). Forage produced under the TG schedule was mostly leaves (93%) with a higher concentration of crude protein (CP; 138 g/kg in the whole forage), a lower concentrations of neutral detergent fibre (NDF) in the stems (740 g/kg), and higher in vitro dry matter digestibility (IVDMD) of the leaves (690 g/kg), compared to the other treatments. Lower grazing frequency strategies (DG and 28-d) resulted in forage with higher proportions of stems (10 and 9%, respectively). Strategies based on light interception did not produce pre-graze forage with a uniform nutritive value, as the indicators varied across grazing cycles. The treatment based on fixed days of rest did not result in uniformity.FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo

Oscillator Strengths and Damping Constants for Atomic Lines in the J and H Bands

We have built a line list in the near-infrared J and H bands (1.00-1.34, 1.49-1.80 um) by gathering a series of laboratory and computed line lists. Oscillator strengths and damping constants were computed or obtained by fitting the solar spectrum. The line list presented in this paper is, to our knowledge, the most complete one now available, and supersedes previous lists.Comment: Accepted, Astrophysical Journal Supplement, tentatively scheduled for the Sep. 1999 Vol. 124 #1 issue. Text and tables also available at http://www.iagusp.usp.br/~jorge

Hot Horizontal Branch Stars in the Galactic Bulge. I

We present the first results of a survey of blue horizontal branch (BHB) stars in the Galactic bulge. 164 candidates with 15 < V < 17.5 in a field 7.5deg from the Galactic Center were observed in the blue at 2.4A FWHM resolution with the AAT 2dF spectrograph. Radial velocities were measured for all stars. For stars with strong Balmer lines, their profiles were matched to theoretical spectrum calculations to determine stellar temperature Teff and gravity log g; matches to metal lines yielded abundances. CTIO UBV photometry then gave the reddening and distance to each hot star. Reddening was found to be highly variable, with E(B-V) from 0.0 to 0.55 around a mean of 0.28. Forty-seven BHB candidates were identified with Teff >= 7250K, of which seven have the gravities of young stars, three are ambiguous, and 37 are HB stars. They span a wide metallicity range, from solar to 1/300 solar. The warmer BHB's are more metal-poor and loosely concentrated towards the Galactic center, while the cooler ones are of somewhat higher metallicity and closer to the center. Their red B-V colors overlap main-sequence stars, but the U-B vs. B-V diagram separates them until E(B-V) > 0.5. We detect two cool solar-metallicity HB stars in the bulge of our own Galaxy, the first such stars known. Still elusive are their hot counterparts, the metal-rich sdB/O stars causing excess UV light in metal-rich galaxies; they have V ~ 20.5 in the Bulge.Comment: 29 pages, 4 figures (the third with 4 panels, the fourth with 2 panels). To appear in the Astrophysical Journal v571n1, Jan. 20, 2000. Abstract is shortened here, and figures compresse

Monte Carlo Simulations of Globular Cluster Evolution - II. Mass Spectra, Stellar Evolution and Lifetimes in the Galaxy

We study the dynamical evolution of globular clusters using our new 2-D Monte Carlo code, and we calculate the lifetimes of clusters in the Galactic environment. We include the effects of a mass spectrum, mass loss in the Galactic tidal field, and stellar evolution. We consider initial King models containing N = 10^5 - 3x10^5 stars, and follow the evolution up to core collapse, or disruption, whichever occurs first. We find that the lifetimes of our models are significantly longer than those obtained using 1-D Fokker-Planck (F-P) methods. We also find that our results are in very good agreement with recent 2-D F-P calculations, for a wide range of initial conditions. Our results show that the direct mass loss due to stellar evolution can significantly accelerate the mass loss through the tidal boundary, causing most clusters with a low initial central concentration (Wo <~ 3) to disrupt quickly in the Galactic tidal field. Only clusters born with high initial central concentrations (Wo >~ 7) or steep initial mass functions are likely to survive to the present and undergo core collapse. We also study the orbital characteristics of escaping stars, and find that the velocity distribution of escaping stars in collapsing clusters looks significantly different from the distribution in disrupting clusters. We calculate the lifetime of a cluster on an eccentric orbit in the Galaxy, such that it fills its Roche lobe only at perigalacticon. We find that such an orbit can extend the lifetime by at most a factor of a few compared to a circular orbit in which the cluster fills its Roche lobe at all times.Comment: 32 pages, including 10 figures, to appear in ApJ, minor corrections onl

Demyelination and axonal preservation in a transgenic mouse model of Pelizaeus-Merzbacher disease

It is widely thought that demyelination contributes to the degeneration of axons and, in combination with acute inflammatory injury, is responsible for progressive axonal loss and persistent clinical disability in inflammatory demyelinating disease. In this study we sought to characterize the relationship between demyelination, inflammation and axonal transport changes using a Plp1-transgenic mouse model of Pelizaeus-Merzbacher disease. In the optic pathway of this non-immune mediated model of demyelination, myelin loss progresses from the optic nerve head towards the brain, over a period of months. Axonal transport is functionally perturbed at sites associated with local inflammation and 'damaged' myelin. Surprisingly, where demyelination is complete, naked axons appear well preserved despite a significant reduction of axonal transport. Our results suggest that neuroinflammation and/or oligodendrocyte dysfunction are more deleterious for axonal health than demyelination per se, at least in the short ter