Impacts of Saharan dust intrusions on bacterial communities of the low troposphere

We have analyzed the bacterial community of a large Saharan dust event in the Iberian Peninsula and, for the frst time, we ofer new insights regarding the bacterial distribution at diferent altitudes of the lower troposphere and the replacement of the microbial airborne structure as the dust event receeds. Samples from diferent open-air altitudes (surface, 100m and 3km), were obtained onboard the National Institute for Aerospace Technology (INTA) C-212 aircrafts. Samples were collected during dust and dust-free air masses as well two weeks after the dust event. Samples related in height or time scale seems to show more similar community composition patterns compared with unrelated samples. The most abundant bacterial species during the dust event, grouped in three diferent phyla: (a) Proteobacteria: Rhizobiales, Sphingomonadales, Rhodobacterales, (b) Actinobacteria: Geodermatophilaceae; (c) Firmicutes: Bacillaceae. Most of these taxa are well known for being extremely stress-resistant. After the dust intrusion, Rhizobium was the most abundant genus, (40–90% total sequences). Samples taken during the fights carried out 15 days after the dust event were much more similar to the dust event samples compared with the remaining samples. In this case, Brevundimonas, and Methylobacterium as well as Cupriavidus and Mesorizobium were the most abundant genera

Identification of intergenerational epigenetic inheritance by whole genome DNA methylation analysis in trios

Abstract Genome-wide association studies have identified thousands of loci associated with common diseases and traits. However, a large fraction of heritability remains unexplained. Epigenetic modifications, such as the observed in DNA methylation have been proposed as a mechanism of intergenerational inheritance. To investigate the potential contribution of DNA methylation to the missing heritability, we analysed the methylomes of four healthy trios (two parents and one offspring) using whole genome bisulphite sequencing. Of the 1.5 million CpGs (19%) with over 20% variability between parents in at least one family and compatible with a Mendelian inheritance pattern, only 3488 CpGs (0.2%) lacked correlation with any SNP in the genome, marking them as potential sites for intergenerational epigenetic inheritance. These markers were distributed genome-wide, with some preference to be located in promoters. They displayed a bimodal distribution, being either fully methylated or unmethylated, and were often found at the boundaries of genomic regions with high/low GC content. This analysis provides a starting point for future investigations into the missing heritability of simple and complex traits

Impacts of Saharan Dust Intrusions on Bacterial Communities of the Low Troposphere

© The Author(s) 2020.We have analyzed the bacterial community of a large Saharan dust event in the Iberian Peninsula and, for the first time, we offer new insights regarding the bacterial distribution at different altitudes of the lower troposphere and the replacement of the microbial airborne structure as the dust event receeds. Samples from different open-air altitudes (surface, 100 m and 3 km), were obtained onboard the National Institute for Aerospace Technology (INTA) C-212 aircrafts. Samples were collected during dust and dust-free air masses as well two weeks after the dust event. Samples related in height or time scale seems to show more similar community composition patterns compared with unrelated samples. The most abundant bacterial species during the dust event, grouped in three different phyla: (a) Proteobacteria: Rhizobiales, Sphingomonadales, Rhodobacterales, (b) Actinobacteria: Geodermatophilaceae; (c) Firmicutes: Bacillaceae. Most of these taxa are well known for being extremely stress-resistant. After the dust intrusion, Rhizobium was the most abundant genus, (40–90% total sequences). Samples taken during the flights carried out 15 days after the dust event were much more similar to the dust event samples compared with the remaining samples. In this case, Brevundimonas, and Methylobacterium as well as Cupriavidus and Mesorizobium were the most abundant genera.This work was funded by the Spanish Economy and Competitiveness Ministry (MINECO) grants CGL2015-69758-P, CGL2017-92086-EXP, ESP2016-79612-C3-1-R, RTI2018-094867-BI00 and i-LINK 1151 grant founded by the Council for Scientific Research (CSIC). FPS was supported by the MINECO grant CTM2016-80095-C2-1-R

Libro Digital Proyectos Posgrados 2015-30

MaestríaDoctoradoDoctor en Ingeniería Eléctrica y ElectrónicaDoctor en Ingeniería CivilDoctor en Ingeniería de Sistemas y ComputaciónDoctor en Ingeniería IndustrialDoctor en Ingeniería MecánicaMagister en Gobierno de Tecnología InformáticaMagister en Ingeniería AdministrativaMagister en Ingeniería AmbientalMagister en Ingeniería CivilMagister en Ingeniería de Sistemas y ComputaciónMagister en Ingeniería EléctricaMagister en Ingeniería ElectrónicaMagister en Ingeniería IndustrialMagister en Ingeniería Mecánic