A comparative study between X-chromosome mapping of Sudanese and Egyptian Anopheles pharoensis theobald (Diptera: Culicidae) strains

Background: Current literature lacks information regarding Anopheles pharoensis malaria transmission patterns. Anopheles pharoensis succeeded in transmitting Malaria in Egypt. However, it was unsuccessful in doing the same in Sudan. From here arises these important questions: Why does it transmit malaria in Egypt but not in the Sudan or other Countries? Is it a Sibling species or a Sub-species? This investigation aimed to answer these questions by studying the genetics of Anopheles pharoensis collected from Egypt and Sudan.Methods: A comparative study was conducted in Egypt and Sudan to determine the population genetic structure of Anopheles pharoensis species based on chromosomal inversion of karyotypes. Fourth-stage larvae of Anopheles pharoensis were collected from Egypt (Faiyoum government) and from Sudan (Khartoum, Gezira and Sennar states).Result: Significant levels of differentiation were observed among the species studied.Conclusion: Investigations suggest that Anopheles pharoensis species collected from Egypt can transmit malaria due to the presence of an inversion in the X-chromosome. The lack of the aforementioned inversion probably prevented the transmission of malaria by Anopheles pharoensis in Sudan. The results above provide insight into malaria transmission patterns by Anopheles pharoensis. However, more needs to be done and hence it is recommended to undergo further research on genetics and morphological studies using molecular biological tool son Anopheles pharoensis in Sudan

Identification and quantification of quercetin, a major constituent of Artocarpus altilis by targeting related genes of apoptosis and cell cycle: in vitro cytotoxic activity against human lung carcinoma cell lines

Nine phenolic compounds were identified and quantified in Artocarpus altilia fruit. One of the main compounds was quercetin, which is the major class of flavonoids has been identified and quantified in pulp part of A. altilis fruit of methanol extract. The aim of this study was to evaluate in vitro cytotoxic assay. Inhibitory concentration 50% concentration was determined using trypan blue exclusion assay. Apoptosis induction and cell cycle regulation were studied by flow cytometric analysis. The expression of apoptosis and cell cycle-related regulatory genes were assessed by RT-qPCR study of the methanol extract of pulp part on human lung carcinoma (A549) cell line. A significant increase of cells at G2/M phases was detected (P<0.05). Furthermore, the pulp of the fruit downregulated the expression of antiapoptosis gene BCL-2 and upregulated the expression of pro-apoptosis gene BAX. CASPASE3 was also activated by the fruit, which started a CASPASE-3-depended mitochondrial pathway to induce apoptosis. As the results, the pulp was the most active in terms of all tests, due to high amount of quercetin in pulp part, 78% of total flavonoids. Taken together, these findings suggested that A. altilis induces apoptosis in a mitochondrial-dependent pathway by releasing and upregulating CYTOCHROME C expression and regulates the expression of downstream apoptotic components, including BCL-2 and BAX