Determining Vitamin D Status: A Comparison Between Diabetic And Non-diabetic Women With Breast Cancer By RP-HPLC

Background: Suboptimal levels of Vitamin D, remains a common problem worldwide and its prevalence is high in India, ranging from 70-100%. Several molecular mechanisms have been found about the effects of Vitamin D in modulating glycemic levels and its protective nature in the development of breast cancer. Breast cancer in India is a common problem and studies regarding its association with Vitamin D levels among diabetics remain inconclusive. This study aims to find the association between Vitamin D status and breast cancer in diabetic and non-diabetic women. Materials and methods: 25 hydroxy vitamin D levels were estimated by RP-HPLC. A total of 25 serum samples were analysed (20 Breast cancer patients+5 normal). Results: Serum sample analysis report of breast cancer patients (irrespective of diabetic status) showed 50% low Vitamin D compared to normal individuals. Conclusions: Vitamin D deficiency is very common among women suffering from breast cancer. Although diabetic breast cancer women have lower Vitamin D levels when compared to non-diabetic women, the results in this study are not statistically significant

Phytochemical fractions from Annona muricata seeds and fruit pulp inhibited the growth of breast cancer cells through cell cycle arrest at G(0)/G(1) phase

Introduction: Annona muricata (L.) (AM), commonly known as Soursop and Lakshmanaphala/Hanumaphala in India, has been extensively used in ethnomedicine for treating tuberculosis, urinary tract infections (UTIs) and cancers. The fruit is a rich source of antioxidants and antitumor agents. Methods: In this study, we have extracted phytochemicals that exhibited anti-cancer property from the (a) fruit pulp using methanol (AMPM) and water (AMPW); and (b) seeds using methanol (AMSM). Qualitative phytochemical analysis showed the presence of phenolics, tannins, alkaloids, flavonoids, sterols, terpenoids, carbohydrates and proteins in AMPM and AMPW. All three extracts were first checked for in vitro antioxidant and anti-inflammatory properties and then tested for efficacy against MCF-7 and MDA-MB-231. Results: Among these three extracts, AMSM showed the highest antioxidant power as well as similar to 80% inhibition at 320 mu g/ml concentration in both cell lines upon treatment for 24h. However, only about 40% inhibition was observed with 320 mu g/ml AMPM treatment, despite its highest anti-inflammatory potential. Water extract AMPW exhibited about 80% growth inhibition at 50% dilution. Since fruit pulp is the one consumed, the extracts AMPM and AMPW were further tested for apoptosis induction and cell cycle arrest. Analysis of the data showed increased apoptosis and G0/G1 cell cycle arrest upon exposure to AMPM and AMPW