Hemolysis and ATP release from human and rat erythrocytes under conditions of hypoxia: a comparative study

Red blood cells are involved not only in transportation of oxygen and carbon dioxide but also in autoregulation of vascular tone by ATP release in hypoxic conditions. Molecular mechanisms of the ATP release from red blood cells in response to a decrease in partial oxygen pressure still remain to be elucidated. In this work we have studied effects of hypoxia on red blood cell hemolysis in humans and rats and compared the effects of inhibitors of ecto-ATPase and pannexin on the release of ATP and hemoglobin from rat erythrocytes. The 20-min hypoxia at 37°C increased hemolysis of red blood cells in humans and rats 1.5- and 2.5-fold, respectively. In rat erythrocytes a significant increase in hypoxia-induced extracellular ATP level was found only in the presence of ecto-ATPase inhibitor ARL 67156. In these conditions we observed a positive correlation (R2 = 0.5003) between the increase in free hemoglobin concentration and the ATP release. Neither carbenoxolon nor probenecid, the inhibitors of low-selectivity pannexin channels, altered the hypoxia-induced ATP release from rat erythrocytes. The obtained results indicate a key role of hemolysis in the ATP release from red blood cells

The Lower Cambrian of the Salair and Gorny Altai (Siberia) revisited

This paper discusses the fossil record, stratigraphy and sedimentary environments of lower Cambrian sequences that crop out in the Salair and Gorny Altai, southern part of western Siberia (Russia). Numerous and well-preserved archaeocyaths and a few small shelly fossils (SSF) have been discovered in carbonate sequences that crop out nearby the Gavrilovka village, Salair. The presence of the Gordonicyathus howelliarchaeocyath Zone is established for the first time in this thick sequence of biogenic carbonates, in addition to the Nochoroicyathus mariinskii Zone, which was known previously from the Gavrilovka Formation. Both of these zones are Atdabanian in age. It is likely that accumulation of the Gavrilovka Formation took place in relatively shallow waters, along reef buildups surrounded by lagoons with archaeocyaths. In the Ak-Kaya section of the Gorny Altai, the trilobite species Alacephalus contortus and Poliellaspis rotundata, known previously from the upper Atdabanian, were discovered, only a few meters above horizons bearing lower Botoman trilobites. We may consider that the previously known age range of these two species is slightly longer and should be extended up to the lower Botoman. Alternatively, on the basis of their paleoecological affinity for agitated waters, these trilobites may be interpreted as reworked in lower Botoman strata

Hemolysis and ATP release from human and rat erythrocytes under conditions of hypoxia: a comparative study

Red blood cells are involved not only in transportation of oxygen and carbon dioxide but also in autoregulation of vascular tone by ATP release in hypoxic conditions. Molecular mechanisms of the ATP release from red blood cells in response to a decrease in partial oxygen pressure still remain to be elucidated. In this work we have studied effects of hypoxia on red blood cell hemolysis in humans and rats and compared the effects of inhibitors of ecto-ATPase and pannexin on the release of ATP and hemoglobin from rat erythrocytes. The 20-min hypoxia at 37°C increased hemolysis of red blood cells in humans and rats 1.5- and 2.5-fold, respectively. In rat erythrocytes a significant increase in hypoxia-induced extracellular ATP level was found only in the presence of ecto-ATPase inhibitor ARL 67156. In these conditions we observed a positive correlation (R2 = 0.5003) between the increase in free hemoglobin concentration and the ATP release. Neither carbenoxolon nor probenecid, the inhibitors of low-selectivity pannexin channels, altered the hypoxia-induced ATP release from rat erythrocytes. The obtained results indicate a key role of hemolysis in the ATP release from red blood cells