Bone Regeneration in the Application of a New Device for Osteosynthesis in the Experiment

Background. Treatment of residual cavities after radical surgery for bone diseases is a vital problem of modern traumatology. Currently, there is an active search for devices both for osteosynthesis and bone-substituting masses. Aims. The purpose of this work was to test a new device for osteosynthesis. It can be used both for fixing bone fragments, and for studying the morphological features of bone regenerate in experiment.Materials and methods. A device for bone osteosynthesis of tubular bones in an experiment (Patent of the Russian Federation N 20116121487) was applied. The device consisted of two half-bushings, fastened to each other by a fierce clamp. Polypropylene was used as the material for the half-bushings, therefore no immunological reactions were observed. On the inner surface there were silicone cylinders, which directed pressure on the fragments and did not disturb the nutrition of the periosteum. The experiment was performed on Wistar rats. The surgical defect of the femur was filled with hydroxyapatite. Two groups of animals were identified. The bone in the first group was fixed by the device. The wire cerclage was used in the second group.Results. The study showed that the developed device does not disturb the nutrition of the periosteum, and also tightly fixes the osteoplastic material in the bone defect. Histological examination showed that bone regeneration was faster when the device was used. On the 21st day of the experiment, the regenerate filled the defect in the control group by 62 %, and in the experimental group by 73 %. The regenerate was represented by a woven bone. In all animals, bone regenerate formed a strong osteo-integrative connection with the maternal bone. In both groups, it looked like a mature bone tissue at the end of the experiment (28 days).Conclusion. The data indicate that the device does not disturb the processes of bone formation and allows for more efficient use osteoplastic material

Side effects of statins in patient with compensated hypothyroidism and SLCO1B1 *5 (c.521T>C) polymorphism

Aim: to assess the influence of compensated hypothyroidism and SLCO1B1 *5 (c.521TC) gene polymorphism on the clinical and laboratory signs of the muscle damage during statin therapy. Methods: assessment of symptoms and markers of the muscle damage and SLCO1B1 *5 (c.521TC) genotyping were performed in 33 patients with primary hypothyroidism taking statins, in 31 patients taking statins without hypothyroidism and in 33 patients with primary hypothyroidism without statins taking. Results: muscle pain was observed more often in the group of the patients with compensated hypothyroidism on the background of statins taking compared with other groups (45,5, 16,1 and 30,3 %, respectively, p=0,048). Only in this group the pain was associated with increased levels of creatine- kinase (171,0108,12 and 110,043,81U/L, in the presence and absence of the pain, p=0,049), LDH (369,566,22 and 305,641,98 U/L, р=0,007), myoglobin titer (90,7109,89 and 41,128,56, р=0,005), and more frequent occurrence of TC and CC genotypes of SLCO1B1*5 (c.521TC) (68,4 и 28,6%, р=0,0027). Conclusions: the patients with compensated hypothyroidism have a higher risk of statin-induced myopathy increasing if the TC heterozygotes or CC homozygotes of SLCO1B1 *5 (c.521TC) gene are present, which requires thorough monitoring of clinical and biochemical muscle damage signs in case of its detection