2 research outputs found
A new copper(I) coordination polymer from 2,6-bis(1<i>H</i>-benzotriazol-1-ylmethyl)pyridine: Synthesis, characterization, and use as additive in transparent submicron UV filters
<p>The use of a new copper(I) coordination polymer (CP) as additive in transparent composite films of 190 nm of thickness for ultraviolet (UV) shielding is presented. The luminescent 1-D Cu(I) CP was easily synthesized through a self-assembly process between Cu(I) iodide and 2,6-bis(1<i>H</i>-benzotriazol-1-ylmethyl)pyridine (L). The CP, [Cu<sub>2</sub>(<i>μ</i> − I)<sub>2</sub>(<i>μ</i> − <b>L</b>)<sub>2</sub>]<sub><i>n</i></sub>, was structurally characterized by infrared, UV–visible diffuse reflectance and photoluminescence spectroscopy, elemental and thermogravimetric analyses, single-crystal and powder X-ray diffraction, and relativistic density functional theory calculations. The CP was dispersed and immobilized into a polymeric matrix in the presence of Sudan I, yielding a composite material that exhibits a reduction of 49% of the UV transmittance at 350 nm. Thus, the use of a new Cu(I) CP in polymeric composite films appears as a novel approach toward ultrathin and transparent UV shielding films, which have potential applications as protection layers of paints and coatings that tend to degrade when exposed to UV radiation.</p
Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids
Glycoengineered bacteria have emerged as a cost-effective
platform
for rapid and controllable biosynthesis of designer conjugate vaccines.
However, little is known about the engagement of such conjugates with
naı̈ve B cells to induce the formation of germinal centers
(GC), a subanatomical microenvironment that converts naı̈ve
B cells into antibody-secreting plasma cells. Using a three-dimensional
biomaterials-based B-cell follicular organoid system, we demonstrate
that conjugates triggered robust expression of hallmark GC markers,
B cell receptor clustering, intracellular signaling, and somatic hypermutation.
These responses depended on the relative immunogenicity of the conjugate
and correlated with the humoral response in vivo. The occurrence of these mechanisms was exploited for the discovery
of high-affinity antibodies against components of the conjugate on
a time scale that was significantly shorter than for typical animal
immunization-based workflows. Collectively, these findings highlight
the potential of synthetic organoids for rapidly predicting conjugate
vaccine efficacy as well as expediting antigen-specific antibody discovery