Full length genome sequence of Polish isolate of Beet soil-borne virus confirms low level of genetic diversity

The complete nucleotide sequence of a Polish isolate of Beet soil-borne virus was determined for the first time. The genome organization was identical with those previously established for isolates from Germany and China. A comparison of the Polish isolate with others deposited in GenBank reveled high level of nucleotide identity, about 98-100%, throughout the genome analyzed. The ratio between non-synonymous and synonymous substitutions was rather low suggesting a negative selective pressure. The non-synonymous mutations were particulary frequent in triple gene block

Transmission rate of two Polish Tomato torrado virus isolates through tomato seeds

Efficient dispersion of Tomato torrado virus (ToTV), an emerging virus affecting tomato crops worldwide, is linked to its principal vectors, Bemisia tabaci and Trialeurodes vaporariorum. To evaluate whether ToTV can be seed-transmitted, we collected seeds from tomato varieties Beta Lux, Grace and Money Maker that had been mechanically inoculated with two ToTV isolates; seedlings were grown in an insect-proof glasshouse, then tested for ToTV by immunocapture-qRT-PCR. Among 17,985 plants, 69 were ToTV-positive (0.38% overall vertical transmission). Bioassays using indicator plants confirmed the presence of infectious virus in the seedlings. This is the first report of tomato seed transmission of ToTV.This study was supported by project N N310 732040 from National Science Centre of Poland. Work in Valencia was supported by grant BFU2015-65037-P from Spain’s Agencia Estatal de Investigación – FEDER.Peer reviewe

Deciphering the Molecular Mechanism of Spontaneous Senescence in Primary Epithelial Ovarian Cancer Cells

Spontaneous senescence of cancer cells remains a puzzling and poorly understood phenomenon. Here we comprehensively characterize this process in primary epithelial ovarian cancer cells (pEOCs). Analysis of tumors from ovarian cancer patients showed an abundance of senescent cells in vivo. Further, serially passaged pEOCs become senescent after a few divisions. These senescent cultures display trace proliferation, high expression of senescence biomarkers (SA-β-Gal, γ-H2A.X), growth-arrest in the G1 phase, increased level of cyclins D1, D2, decreased cyclin B1, up-regulated p16, p21, and p53 proteins, eroded telomeres, reduced activity of telomerase, predominantly non-telomeric DNA damage, activated AKT, AP-1, and ERK1/2 signaling, diminished JNK, NF-κB, and STAT3 pathways, increased formation of reactive oxygen species, unchanged activity of antioxidants, increased oxidative damage to DNA and proteins, and dysfunctional mitochondria. Moreover, pEOC senescence is inducible by normal peritoneal mesothelium, fibroblasts, and malignant ascites via the paracrine activity of GRO-1, HGF, and TGF-β1. Collectively, pEOCs undergo spontaneous senescence in a mosaic, telomere-dependent and telomere-independent manner, plausibly in an oxidative stress-dependent mechanism. The process may also be activated by extracellular stimuli. The biological and clinical significance of pEOC senescence remains to be explored

Correction: Pakuła et al. Deciphering the Molecular Mechanism of Spontaneous Senescence in Primary Epithelial Ovarian Cancer Cells. <i>Cancers</i> 2020, <i>12</i>, 296

In the original publication [...

Correction: Paku&#322;a et al. Deciphering the Molecular Mechanism of Spontaneous Senescence in Primary Epithelial Ovarian Cancer Cells. Cancers 2020, 12, 296

In the original publication [...