Adherence to the American College of Surgeons (ACS) recommendation on double gloving, hand free zone and blunt suture needle use among Surgeon ranks

Background: The American College of Surgeons guidelines suggest the use of intact gloves, double gloving, hands-free zone technique to pass sharp instruments, and blunt tip suture needles to protect patients, as well as the surgical team. This study estimates the extent to which these guidelines are followed in a large academic health system. Methods: Over a two-month period in the spring of 2010, 320 general surgical attendings, subspecialty surgical attendings, and surgical resident physicians practicing at a large academic health system, were approached during or after surgical conferences to participate in a cross-sectional study. Nearly 1/3rd completed an anonymous and voluntary self-administered survey. The survey included questions regarding knowledge of each technique, beliefs about effectiveness of each technique, and adherence to the guidelines. Responses were compared by surgeon rank. Results: Awareness of ACS recommendation guidelines was high among surgical attendings (68%) and residents (60%). While 60% of residents adhered to these recommendations, only 43% of attendings adhered. Both attendings (65%) and residents (64%) had similar negative perception toward double gloving in terms of tactile sensation and hand free zone hindrance during procedural operations during cases. Forty percent of residents and attendings agreed on unhindered concentration to hand free zone technique. Blunt tip suture needle use had low awareness and usage regardless of surgeon rank (~40%). Conclusion: Increased promotion of the ACS guidelines is warranted. Continuing medical education for surgical attendings may promote more widespread adoption of techniques to promote safety

Characterization and Sequencing of MT-Cox1 Gene in Khorasan Native Poultry

The aim of this study was to investigate the nucleotide sequence of COX1 gene in mitochondrial genome of Khorasan native chicken and detect the possible mutations in the genome. For this purpose, after sampling and extracting DNA from the whole blood samples, the COX1 gene was amplified using specific primers and cloned in pTG19-T plasmid of the DH5α strain of Escherichia coli bacteria. Finally, the recombinant plasmids were extracted from positive colonies and sequenced. The results of nucleotide sequence indicated maximum of similarity with the same sequence in the Gallus gallus mitochondrial genome. Comparing these two sequences identified the presence of six nucleotide differences. However, only three point mutations led to the altering of dissimilar amino acids. Also, a protein BLAST query showed that the sequence had a similarity of 99% in 100% coverage to cytochrome C oxidase subunit 1. The protein BLAST query suggested the conserved domain cytochrome C, in a family which is part of a complex IV. This complex converts the oxygen into the water by electrons transfer from reduced cytochrome in a reaction associated with mitochondrial electron transport chain. The findings may be useful in identifying phenotypes in poultry.Keywords: Cloning, Sequencing, MT-COX1, Khorasan native poultry, Gallus gallu

Supply Chain Management and Service Quality in Malaysian Hotel Industry

Studying supply chain management has been receiving numerous attentions. In the hospitality industry attempt have been made to study this concept, but more enquiries need to be conducted. Along with this objective, this study investigated the role of supply chain management practices in enhancing service quality in the hotel industry in Malaysia, which is a favorite destination nowadays. Analyzing 89 data from managers in hotel industry in Malaysia revealed that successful supply chain management practices would lead to higher service quality. Keywords: Strategic purchasing, Communication, Suppliers’ relationship, Service quality, Hotel industry

Palladium(II) saccharinate complexes trans-[Pd(sac)(2)(LH)(2)] with amino- and acetylamino-pyridine co-ligands: molecular structures of trans-[PdCl2(2-ampyH)(2)].2dmf (2-ampyH=2-amino-3-methylpyridine) and trans-[Pd(kappa(2)-2-acmpy)(2)] (2-acmpyH=2-acetylamino-3-methylpyridine)

Reaction of Na2[PdCl4] with two equivalents of amino- or acetylamino-pyridines (LH) affords trans-[PdCl2-(LH)2] {LH = 2-amino-3-methylpyridine (2-ampyH), 3-aminopyridine (3-apyH), 2-acetylamino-3-methylpyridine (2-acmpyH), 3-acetylamino-pyridine (3-acpyH)}. An X-ray crystal structure of trans-[PdCl2(2-ampyH)2] shows that the 2-ampy-H ligands are coordinated in a monodentate fashion via the nitrogen atoms of the pyridine rings. Treatment of trans-[PdCl2(2-acmpyH)2] with NEt3 affords the cyclometalated complex, trans-[Pd(κ2-2-acmpy)2], the X-ray structure of which shows that the 2-acmpy ligand is coordinated to palladium in a bidentate fashion via the nitrogen atom of the pyridine ring and oxygen. Reaction of trans-[PdCl2(LH)2] with two equivalents of sodium saccharinate affords the bis(saccharinate) complexes, trans-[Pd(sac)2(LH)2], in which the saccharinate anions are coordinated via the amide nitrogen atom

Implication of complex vertebral malformation and deficiency of uridine monophosphate synthase on molecular-based testing in the Iranian Holstein bulls population

The aim of this study was to identify the deficiency of uridine monophosphate synthase (DUMPS) and the complex vertebral malformation (CVM) in Iranian Holstein bulls. A total of 144 blood samples were prepared of Holstein bulls in Abbas Abad Animal Breeding Center and Ferdowsi University of Mashhad's Dairy Farm in Khorasan state of Iran. Genomic PCR-RFLP protocol was performed to amplify the polymorphic region of the bovine uridine monophosphate synthase UMPS gene. Also, genomic PCR-SSCP method was performed for CVM to amplify the polymorphic region of the bovine solutecarrier family 35 member 3 (SLC35A3) genes. The results of this study demonstrated that there was no carrier of DUMPS and CVM in Iranian bulls in these centers

The diversity of leptin gene in Iranian native, Holstein and Brown Swiss cattle

This study describes genetic variability in the leptin in Iranian native, Brown Swiss and Holstein cattle (Bos Indicus and Bos Taurus). This is the first study of genetic polymorphism of the leptin gene in Iranian native cattle. We examined exon 2 of the leptin gene from 587 individuals in six different populations of Iranian native cattles (86 Sarabi, 66 Taleshi, 94 Sistani, 76 Golpayegani, 104 Brown Swiss and 161 Holstein cattle) using PCR-RFLP method. Analysis of the frequencies of the various alleles ineach breed indicated that allele C in Sarabi, Taleshi, Sistani, Golpayegani, Brown Swiss and Holstein cattle with 68, 55, 69, 71, 55 and 57% value were the most frequent alleles. Observed heterozygosities were highest in Golpayegani (57.89%). These new data suggest that allele frequencies of leptin differ between the various Iranian cattle breeds

Genetic Diversity of Urial Population in Northeast of Iran

Habitat eradication and loss of animal species have created a new international hazard for wildlife conservation. National parks are considered as suitable places that can serve dual functions of biodiversity conservation and ecotourism. As recommended by the Food and Agriculture Organization (FAO), microsatellites have been used for animal biodiversity assessment. For this reason, Iranian urials population genetic diversity was studied by analyzing of 10 microsatellite markers in 75 skeletal muscle samples that were collected from Tandooreh National Park, Northeastern of Iran. Species of samples validated by sequencing of the control region from mtDNA. Allelic frequencies for each locus in the population and different measurements of within-breed genetic variations were computed by the POPGENE32 software. The number of alleles per locus counted from 5 to 8, with an average of 6.1. The polymorphism information content was calculated between 0.66-0.74 with the average of 0.7. Observed heterozygosity ranged from 0.223 (MaF214) to 0.776 (OarFCB128) with an average about 0.584 while the average expected heterozygosity for all studied loci was 0.785 ranging from 0.765 (BM8125) to 0.807 (MaF36). High levels of expected heterozygosity can be attributed to some factors such as low level of inbreeding, low selection pressure, and high allele number. However, findings of the present study of the high variability of the Iranian urials showed the presence of a possible ‘hot spot’ genetic diversity for wild urial population in the Northeast of Iran. In conclusion, values of genetic diversity revealed that the Iranian urial population harbor unique and appreciable reservoirs of diversity

Bioinformatics Analysis of Upstream Region and Protein Structure of Fungal Phytase Gene

Phytase increases the bioavailability of phytate phosphorus in seed-based animal feeds and reduces the phosphorus pollution of animal waste. Since most animal feeds for pellets are heated up to 65-80 °C, the production of a thermostable structure for phytase can be useful. In this study, we sought to perform bioinformatics analysis of the upstream region and protein structure of fungal phytase to improve its expression and thermostability properties. We used bioinformatics methods such as similarity search, multiple alignment, statistical analysis of physicochemical properties of amino acids, pattern recognition, and protein modeling to find out the effective factors in heat resistance of phytase. Change in Gibbs free energy (ΔG) of the best pattern promoter resulting from the interaction between RNA polymerase and the promoter sequences of modified genes of phytase was equal to -9 kcalmol-1, which is lower compared to other interactions. The evaluation of the three-dimensional structure of new phytases showed that amino acid substitutions aimed at improving thermostability did not change the form and structure of the protein. The results of Prochek, Whatcheck, and ERRAT for structural analysis and verification were 84, 72, and 70, respectively, that were satisfactory

Perceived barriers and policy solutions in clean energy infrastructure investment

International political negotiations and national policy for climate change mitigation are increasingly focussed on the mobilisation and scale up of investments in clean energy infrastructure. This paper aims to develop the understanding of how institutional investors in the private sector perceive barriers to scaling up investment into clean energy infrastructure and what policy solutions to those barriers they advocate. This paper adds to existing scientific knowledge through a clear focus on private sector perceptions. Through the analysis of previous public statements from organisations and coalitions in the finance sector a number of investment barriers were identified. These initial barriers fed into a Delphi process. The outputs of the Delphi process were categorized into five sets of barriers and a number of policy solutions associated with investing into clean energy solutions. We conclude that there is a need for better engagement with the institutional investment community to ensure further effort on policy development that underpins investments at scale is effective and efficient