The Effect of Recombinant Human Bone Morphogenetic Protein-7 on the Osteoblast-like Cells Cultured on Implant

AIM: The aim of the study was to study the effect of recombinant human bone morphogenetic protein-7 (rhBMP-7) on the osteoblast-like cells cultured on implant. MATERIALS AND METHODS: The osteoblast-like osteo-1 cell line was used in this experiment and derived from the parietal bone tissue of newborn albino rats. The cells were incubated in a humid atmosphere of 95% air and 5% carbon dioxide at 37°C. The medium was changed every 2 days. Four groups were conducted as follows osteoclast-like cell (machined), implanted osteoclast-like cell on titanium (Ti) (modified), osteoclast-like cell supplemented with BMP-7 (Machined + BMP-7), and implanted osteoclast-like cell on Ti and supplemented with BMP-7 (Modified + BMP-7). RESULTS: Cell proliferation was influenced by rhBMP-7, as demonstrated by a significant increase in collagen content after 7 and 21 days of culture (p = 0.005) and a significant increase in alkaline phosphatase (ALP) activity after 7 days (p < 0.001). The addition of rhBMP-7 influenced ALP activity, and a significant increase was observed after 21 days (p < 0.001). CONCLUSION: Within the limitations of the study, we concluded that the presence of rhBMP-7 did not influence cell adhesion to chemically modified Ti surfaces but provided an additional stimulus during the differentiation of rat osteo-1 cells cultured on this type of surface

Nonlinear Analysis of Circular Plates on Nonlinear Foundation

Galerkin\u27s procedure and the modified energy expression of Banerjee are used to obtain the central deflection of circular plates with linearly varying thicknesses, resting on a linear as well as a nonlinear elastic foundation. A new series formula for the deflection has been considered. The accuracy of the method has been tested for clamped plates with movable and immovable ends under uniform static patch loading. Graphical results are drawn for the central deflection for different end conditions, different plate thicknesses and different foundation properties. Comparison of the results are made with other known results and shows a good agreement in most tested cases

Improving the voltage quality of Abu Hummus network in Egypt

In this paper the performance of the electrical network of Egypt is studied by considering a small part on the network (Abu Hummus city). The transmission network of Abu Hummus city was created for 66 kV, 11 kV, and 0.4 kV in the digital simulation and electrical network calculation (DIgSILENT power factory software) to study the voltage profiles. The load flow operational analysis was performed to obtain the voltage magnitudes at every bus bar. The voltage magnitudes in 11 kV and 0.4 kV networks were 10% to 15% less than the nominal value due to overloading off the transmission lines and the voltage magnitudes in 66 kV was within permissible limits. By using automatic tap-changing transformer or Static VAR System, the main idea of this paper is to obtain the voltage profiles at every bus bar to improve the voltage quality of the networks, so as to achieve better voltage profiles on the low voltage side without much effect on high voltage side under various operating conditions

Treatment of olive mill based wastewater by means of magnetic nanoparticles: Decolourization, dephenolization and COD removal

AbstractOlive mill wastewater (OMW) is an environmental concern that has been highlighted as a serious environmental problem in the Mediterranean basin countries because of its high organic load and phytotoxic and antibacterial phenolic compounds, which resist biological degradation. Consequently, this type of wastewater represents a huge challenge for the conventional wastewater treatment techniques as it can impact the lifetime of bacteria needed for the treatment. Iron-oxide nanoparticles are attractive for wastewater treatment for two important reasons. First, nanoparticles can remove pollutants from wastewater rapidly. Second, this magnetic type of nanoparticles could be separated easily using a magnet after finishing treatment process. In this study, we aimed at investigating the effectiveness of the magnetic iron oxide nanoparticles in the removal of large organic contaminants from OMW. Batch and continuous mode processes were applied on OMW treatment to determine the effect of contact time, solution pH, coexisting contaminants and the adsorption isotherm.The results showed that the adsorption was fast and the adsorption reached equilibrium within less than 30min. The adsorption equilibrium data fit very well to the Brunauer–Emmett–Teller (BET) Model, indicating multi-layers adsorption. The adsorption of major pollutants was associated to an efficient removal of coexisting contaminants such as heavy metals and free ions. The adsorption of OMW pollutants was dependent on pH of the solution. Finally, continuous-mode process was tested successfully using a packed bed column that combined sand filtration with magnetic nanoparticles to decolourize OMW effluent. This study will provide valuable insight on the effect of nanoparticles toward the treatment and recyclability of olive mill wastewater, which is crucial for the local olive mill industry. After seeing the successful achievement of integrating nanoparticles with fixed bed filtration, a preliminary process description and cost estimation of stand-alone plant (with a capacity of 4m3/h) for OMW treatment were considered in this study. Process capital and annual operating costs were estimated to be $12,306 and$476/year, respectively

Secondary metabolites and pharmacology of Foeniculum vulgare Mill

ABSTRACT From hexane extract of Foeniculum vulgare Mill. Subsp. piperitum the fatty acids, hydrocarbons and sterols were identified. The furocoumarins imperatorin, psoralen, bergapten, xanthotoxin and isopimpinellin were isolated from the methylene chloride extract. The flavonoids isorhamnetin 3-O-α-rhamnoside, quercetin and kaempferol were isolated from the ethyl acetate extract, whereas quercetin 3-Orutinoside, kaempferol 3-O-rutinoside and quercetin 3-O-β-glucoside were isolated from the methanol extract. The crude hexane, methylene chloride, ethyl acetate and methanol extracts of this plant showed antinociceptive and anti-inflammatory activity

Rapid detection of equine piroplasms using multiplex PCR and first genetic characterization of Theileria haneyi in Egypt

Equine Piroplasmosis (EP) is an infectious disease caused by the hemoprotozoan parasites Theileria equi, Babesia caballi, and the recently identified species T. haneyi. Hereby, we used a multiplex PCR (mPCR) targeting the 18S rRNA gene of T. equi and B. caballi for the simultaneous detection of EP in Egyptian equids and examined the presence of T. haneyi infections in Egypt. Blood samples from 155 equids (79 horses and 76 donkeys) collected from different governorates of Egypt were examined by mPCR and PCR targeting T. hayeni. The mPCR method revealed a prevalence of T. equi of 20.3% in horses and of 13.1% in donkeys and a prevalence of B. caballi of 1.2% in horses. B. caballi was not detected in donkeys in the current study. The mPCR method also detected coinfections with both species (2.5% and 1.3% in horses and donkeys, respectively). Additionally, we report the presence of T. haneyi in Egypt for the first time in 53.1% of the horse and 38.1% of the donkey tested samples. Coinfection with T. haneyi and T. equi was found in 13.5% of the samples, while infection with the three EP species was found in 1.9% of the samples.B.S.M.E, National research center, (NRC)http://www.mdpi.com/journal/pathogenspm2022Veterinary Tropical Disease

Hepatobiliary manifestations following two-stages elective laparoscopic restorative proctocolectomy for patients with ulcerative colitis: A prospective observational study

BACKGROUNDHepatobiliary manifestations occur in ulcerative colitis (UC) patients. The effect of laparoscopic restorative proctocolectomy (LRP) with ileal pouch anal anastomosis (IPAA) on hepatobiliary manifestations is debated.AIMTo evaluate hepatobiliary changes after two-stages elective laparoscopic restorative proctocolectomy for patients with UC.METHODSBetween June 2013 and June 2018, 167 patients with hepatobiliary symptoms underwent two-stage elective LRP for UC in a prospective observational study. Patients with UC and having at least one hepatobiliary manifestation who underwent LRP with IPAA were included in the study. The patients were followed up for four years to assess the outcomes of hepatobiliary manifestations.RESULTSThe patients' mean age was 36 +/- 8 years, and males predominated (67.1%). The most common hepatobiliary diagnostic method was liver biopsy (85.6%), followed by Magnetic resonance cholangiopancreatography (63.5%), Antineutrophil cytoplasmic antibodies (62.5%), abdominal ultrasonography (35.9%), and Endoscopic retrograde cholangiopancreatography (6%). The most common hepatobiliary symptom was Primary sclerosing cholangitis (PSC) (62.3%), followed by fatty liver (16.8%) and gallbladder stone (10.2%). 66.4% of patients showed a stable course after surgery. Progressive or regressive courses occurred in 16.8% of each. Mortality was 6%, and recurrence or progression of symptoms required surgery for 15%. Most PSC patients (87.5%) had a stable course, and only 12.5% became worse. Two-thirds (64.3%) of fatty liver patients showed a regressive course, while one-third (35.7%) showed a stable course. Survival rates were 98.8%, 97%, 95.8%, and 94% at 12 mo, 24 mo, 36 mo, and at the end of the follow-up.CONCLUSIONIn patients with UC who had LRP, there is a positive impact on hepatobiliary disease. It caused an improvement in PSC and fatty liver disease. The most prevalent unchanged course was PSC, while the most common improvement was fatty liver disease

Serum Apelin and Obesity-Related Complications in Egyptian Children

BACKGROUND: The rapidly increasing prevalence of childhood obesity became a major burden on health worldwide, giving an alarm to clinicians and researchers. Adipocytes act as an active endocrine organ by releasing plenty of bioactive mediators (adipokines) that play a major role in regulating metabolic processes. Apelin is a recently identified adipokine that is expressed in adipocytes.AIM: The current work aimed to uncover the relation between serum apelin and childhood obesity and its related complications as hypertension and hyperglycemiaMETHOD: A group of 50 obese and 31 non-obese; sex- and age-matched children were enrolled in our study with a mean age of (9.5 ± 2.1) and (8.7 ± 1.3) respectively. Anthropometric measurements, blood pressure, were assessed in all studied participants, we also determined the lipid profile, serum insulin, fasting blood glucose (FBG) level, HOMA-IR and serum apelin.RESULTS: Obese children had higher levels of HbA1c, FBG, serum insulin, HOMA-IR, total cholesterol, triglycerides, low-density lipoprotein (LDL) and diastolic blood pressure (DBP Z-score); compared to controls (all P < 0.05). Apelin was significantly higher in obese children versus controls and correlated positively with BMI Z-Score (P = 0.008), DBP Z-Score (P = 0.02), cholesterol, TG (both P = 0.02), serum insulin (P = 0.003), FBG and HOMA-IR (both P = 0.001). Linear regression analysis showed that FBG was the most effective factor in predicting the level of serum apelin (P = 0.04).CONCLUSION: This work supports the hypothesis that apelin may have a crucial role in the pathogenesis of health hazards related to obesity in children including insulin resistance, hypertension and a higher risk of occurrence of metabolic syndrome

Biliary fistula and late recurrence of liver hydatid cyst: Role of cysto-biliary communication: A prospective multicenter study

Background: Hydatid cyst disease (HCD) is common in certain locations. Surgery is associated with postoperative biliary fistula (POBF) and recurrence. The primary aim of this study was to identify whether occult cysto-biliary communication (CBC) can predict recurrent HCD. The secondary aim was to assess the role of cystic fluid bilirubin and alkaline phosphatase (ALP) levels in predicting POBF and recurrent HCD. Aim: To identify whether occult CBC can predict recurrent HCD. The secondary aim was to assess the role of cystic fluid bilirubin and ALP levels in predicting POBF and recurrent HCD. Methods: From September 2010 to September 2016, a prospective multicenter study was undertaken involving 244 patients with solitary primary superficial stage cystic echinococcosis 2 and cystic echinococcosis 3b HCD who underwent laparoscopic partial cystectomy with omentoplasty. Univariable logistic regression analysis assessed independent factors determining biliary complications and recurrence. Results: There was a highly statistically significant association (P ≤ 0.001) between cystic fluid biochemical indices and the development of biliary complications (of 16 patients with POBF, 15 patients had high cyst fluid bilirubin and ALP levels), where patients with high bilirubin-ALP levels were 3405 times more likely to have biliary complications. There was a highly statistically significant association (P ≤ 0.001) between biliary complications, biochemical indices, and the occurrence of recurrent HCD (of 30 patients with recurrent HCD, 15 patients had high cyst fluid bilirubin and ALP; all 16 patients who had POBF later developed recurrent HCD), where patients who developed biliary complications and high bilirubin-ALP were 244.6 and 214 times more likely to have recurrent hydatid cysts, respectively. Conclusion: Occult CBC can predict recurrent HCD. Elevated cyst fluid bilirubin and ALP levels predicted POBF and recurrent HCD

Allelic Discrimination of Vitamin D Receptor Polymorphisms and Risk of Type 2 Diabetes Mellitus: A Case-Controlled Study

(1) Background: Type 2 diabetes mellitus (T2DM) is one of the rapidly growing healthcare problems, and several vitamin D receptor (VDR) polymorphisms seem to modulate the risk of T2DM. Our research was designed to investigate the allelic discrimination of VDR polymorphisms and T2DM occurrence risk. (2) Methods: This case-control research included 156 patients with T2DM and 145 healthy control subjects. Most of the study population were males 56.6% vs. 62.8% in the case and control groups, respectively. Genotyping for VDR single nucleotide polymorphisms (SNPs), rs228570 (Fok1), rs7975232 (Apa1), and rs1544410 (Bsm1) was compared between both groups. (3) Results: There was a negative link between vitamin D levels and insulin sensitivity. A significant difference was noted in the allelic discrimination of VDR polymorphism rs228570 and rs1544410 between the study groups (p \u3c 0.001). No difference was observed in the allelic discrimination of VDR polymorphism rs7975232 between the groups (p = 0.063). Moreover, T2DM patients had significantly higher levels of fasting blood sugar (FBS), glycated hemoglobin HbA1c, 2-h post-prandial blood sugar (PP), serum glutamic oxaloacetic transaminase (SGOT), serum glutamic-pyruvic transaminase (SGPT), total cholesterol, and triglycerides (p \u3c 0.001), while High-Density Lipoprotein (HDL) Cholesterol (HDL-C) was significantly decreased (p = 0.006). (4) Conclusions: VDR polymorphisms had a positive association with T2DM risk among the Egyptian population. Further large-scale research using deep sequencing of samples is strongly urged to investigate different vitamin D gene variants and interactions, as well as the influence of vitamin D on T2DM