Comparative Study of Sodium and Potassium in Different Types of Gallstones and in Serum of Subjects with Gallstones and Controls

The study comprises evaluation of sodium and potassium in the pathogenesis of human gallstones as well as measurement of the concentration of these elements in gallstones and in sera of 109 gallstone subjects and 100 controls (age and sex matched with no personal or family history of gallstone disease). It was observed that serum concentrations for both sodium and potassium were comparable (p>0.05) between gallstone subjects and control subjects. In gallstones the concentration of sodium was significantly higher as compared to potassium (p<0.05). Normal sodium to potassium ratio was seen in serum of gallstone subjects, whereas, low sodium to potassium ratio was seen in gallstone carriers. Amongst the different types of gallstones, significantly high (p<0.05) concentrations of sodium and potassium were seen in calcium bilirubinate gallstones. The levels for these mineral elements were also raised in serum of pure calcium carbonate gallstone subjects.The results demonstrate that the higher concentration of sodium and potassium in gallstones may involve in both calcium bilirubinate gallstones and in serum of calcium carbonate gallstone subjects, which indicate their association with calcium in the precipitation of calcium bilirubinate and calcium carbonate in bile. Furthermore, low sodium to potassium ratio in gallstones indicates low ratio in bile of gallstone subjects

Serum lipid profile as a marker of liver impairment in hepatitis B Cirrhosis patients

Representative GC chromatogram of serum total and free fatty acids of HBV patients and controls along with standards of fatty acid methyl esters.(DOCX 761 kb

Oxidative stress is the common causal link between renal stones and diabetes mellitus in adult people

Objective: To investigate the common risk factors involved in the pathogenesis of renal stones and diabetes mellitus in adults. Method: The case-control study was carried out at the urology outpatient department and diabetic clinic of the Liaquat University of Medical and Health Sciences, Hyderabad, Pakistan, from January 2019 to January 2020, and comprised renal stone patients in group A, diabetes mellitus patients in group B and healthy controls in group C. Height and weight were determined for all subjects, followed by calculation of body mass index. Serum samples were analysed for creatinine, uric acid, total antioxidants, iron, malondialdehyde, superoxide dismutase, catalase, glutathione peroxidase, lactate dehydrogenase, xanthine oxidase, C-reactive protein, and nuclear factor kappa-light-chain-enhancer of activated B cells. Intra-group comparisons were done. Data was analysed using SPSS 22. Results: Of the 400 subjects, 100(25%) each were in groups A and B, and 200(50%) were in group C. Overall, there were 236(59%) males and 164(41%) females. The age range of the sample was 20-40 years. Obesity was more prevalent in group B 26(26%) as against 4(4%) in group A and 20(10%) in group C. Compared to group C, superoxide dismutase (p=0.0128) and C-reactive protein (p=0.032) levels were higher in group B, while the levels were lower for uric acid (p=0.0067), iron (p=0.0147) and xanthine oxidase (p=0.0360). ---Continu

Financování programů reprodukce majetku s účastí státního rozpočtu

Import 20/04/2006Prezenční výpůjčkaVŠB - Technická univerzita Ostrava. Fakulta hornicko-geologická. Institut ekonomiky a systémů řízení (545

Serum lipids as an indicator for the alteration of liver function in patients with hepatitis B

Abstract Background Hepatitis B virus (HBV) exerts an intense impact on host lipid metabolism. Hence the aim of present study is to determine metabolic derangement that occurred in subjects suffering from hepatitis B patients. Methods The fasting blood samples were collected from hepatitis B patients (n = 50) attended in Taluka hospital TandoAdam, Sindh with age and gender matched controls (n = 50). Serum lipid profile and fatty acid (FA) composition were analyzed by micro-lab and gas chromatography. Results The hepatitis B patients have significantly lower level (p < 0.01) of lipid profile including total cholesterol (TC), triacylglyceride (TAG), high density lipoprotein-C (HDL-C) very low density lipoprotein-cholesterol (VLDL-C), low density lipoprotein-cholesterol (LDL-C), and total lipid (TL) in comparison to controls, indicating hypolipidemia in patients. The result of total FA composition of HBV patients in comparison to controls reveal that myristic, palmitic, docosahexaenoic acids were significantly (p < 0.05) higher, while linoleic, eicosatrienoic, arachidonic, eicosapentaenoic acids were lower in HBV patients in comparison to controls. The elongase, ∆5 and ∆6-desaturase enzymes activities were found lower, while ∆9-desaturase activity was higher in hepatitis B patients as compared to controls, which indicates the impaired lipid metabolism. Conclusion The serum saturated fatty acid (SFA) and monounsaturated fatty acid (MUFA) were increased while polyunsaturated fatty acid (PUFA) was reduced in both total and free form in hepatitis B patients due to altered activities of enzyme desaturases with impaired PUFA metabolism and non-enzymatic oxidation

Blood Chemistry (Hematology) of Transfusion Dependent Beta Thalassemia Patients at Hyderabad and Adjoining Areas

Present study was carried out to analyze quantitatively the blood parameters of Transfusion Dependent Thalassemia (TDT) patients. A total number of seventy one (n=71) TDT patients aged 04 to 26 years were selected from Fatimid foundation center, Hyderabad from June 2015 to May 2016 and eighty four (n=84) control subjects (age, gender and locality matched persons with negative personal and family history of thalassemia) were included in this study. Blood sample of 5mL was collected from each participant to analyze white blood cells (WBC); total leukocytes counts (TLC); red blood cells (RBC); mean corpuscular value (MCV); mean corpuscular hemoglobin (MCH); mean corpuscular hemoglobin concentration (MCHC); hemoglobin (HB) and hematocrit (HCT). All the patients and control subjects were interviewed through a standard questionnaire specially developed to study their life style and dietary habits. The collected data from TDT patients and control subjects was statistically analyzed by using p<0.05 as statistically significant variation between two groups. Results revealed significantly decreased levels of HCT, MCV, MCH and platelets in TDT patients as compared to control subjects. All TDT patients were frequently undergoing blood transfusion within the age group